Comparative study of in-vitro antioxidant and antidiabetic activity of plant extracts of Acacia arabica, Murraya koeingii, Catharanthus roseus and Rouwolfia serpentina”

Keshaw Ram Aadil, Anand Barapatre, Namrata Rathore, Sandhya Pottam, Harit Jha


Medicinal plants play very important role in preventing the progress of disease. In present studyfour different medicinal plants, Acacia arabica, Murraya koeingii, Catharanthus roseus and Rouwolfia serpentina commonly found in Chhattisgarh, (India), were screened for the potency of antioxidant and antidiabetic activity. Methanolic and aqueous extracts of A. arabica and M. koeingii, C. roseus and R. serpentina plant leaves were examined. Total phenolic content of extracts was analyzed by Folin-ciocalteu’s method. Antioxidant activities of different extracts were evaluated by DPPH and H2O2 scavenging assay and putative Antidiabetic activity was determined by in-vitro glucose diffusion and alpha-amylase inhibition assay. The highest DPPH scavenging activity was found in methanolic extract of C. roseus while the highest hydrogen peroxide scavenging activity was found in aqueous extract of M. koeingii. In in-vitro antidiabetic test, highest α-amylase inhibition was found in methanolic extract of R. serpentina and the highest diffusion rate of glucose was found in aqueous extract of R. serpentina. The plant extracts were further characterized by Liquid chromatography mass spectroscopy. The results suggest that these plants could serve as a source of natural antioxidants and antidiabetic agents with potential applications in pharmaceuticals industry.


Antioxidant; Acacia arabica; Murraya koeingii; Catharanthus roseus;

Full Text:



.Chen JH and Ho CT. Antioxidant activities of caffeic acids and its related hydrocinnamic acid compounds. Journal of Agricultural and Food Chemistry 1997, 45: 2374–2378.

.Cuyckens F and Claeys. Mass spectrometry in the structural analysis of flavonoids. J. Mass Spectrom 2004, 39:1-15.

.Khosh F and Khosh M. Natural approach to hypertension. Altern Med Rev 2001, 6(6):590-600.

.Anderson KJ, Teuber SS, Gobeille A, Cremin P, Waterhouse AL, & Steinberg FM. Walnut polyphenolics inhibit in vitro human plasma and LDL oxidation. Biochemical and molecular action of nutrients. Journal of Nutrition 2001, 131: 2837–2842.

.Kavishankar GB, Lakshmidevi N, Murthy, M Prakash HS, Niranjana SR. Diabetes and medicinal plants-A review. Int J Pharm Biomed Sci 2011, 2(3): 65-80.

.Modak M, Dixit P, Lodhe J, Ghaskadbi S, Devasgayam TPA. Indian Herbs and herbal drugs used for the Treatment of Diabetes. J Clin Biochem Nut 2007, 40(3):163-173.

.Mishra KP, Ganju L, Sairam M, Banerjee PK, Sawhney RC, A review of high throughput technology for the screening of natural products. Biomed Pharmacother 2008, 62: 94–98.

.Porter JR, Barrett TG. Monogenic syndromes of abnormal glucose homeostasis:clinical review and relevance to the understanding of the pathology of insulin resistance and b cell failure. J Med Genet 2005, 42:893–902.

.Keshavkant S, Sukhdev T, Srinivasarao C, Naithani SC. Antioxidant activities, phenols and flavonoid contents of Withania somnifera and Rauwolfia serpentine, Indian Journal of Plant Physiology 2008,13(4):394-399.

.Kumbhare MR, Guleha V2, Sivakumar. T2 Estimation of total phenolic content, cytotoxicity and in-vitro antioxidant activity of stem bark of Moringaoleifera. Asian Pacific Journal of Tropical Disease 2012, 144-150.

.Lee Y, Kim H, Choi HS, Kang BH, Han YB, Kim SJ. “Effects of water extract of 1:1 mixture of Phellodendron cortex and Aralia cortex on polyol pathway and oxidative damage in lenses of diabetic rats. Phytother Res 1999,13(7): 555-560.

.Saha MR, Hasan SMR, Akter R, Hossain MM, Alam MS, Alam MA, Mazumder MEH. In vitro free radical scavenging activity of methanol extracts of the leaves of Mimusops elengi Linn. Bangladesh J. Vet. Med. 2008, 6:197–202.

.Imran M, Raja MM, Barith A, Asarudeen A. Determination of total phenol, flavonoid and antioxidant activity of edible mushrooms Pleurotusflorida and Pleurotuseous. International Food Research Journal 2011, 18:579-582.

.Sangala R, Kadati DR, Burra SD, Gopu J, Dubasi A. Evaliuation of Antidiabetic Activity of Annona Squamosa Linn seed in Alloxan-Induced Diabetic Rats. International Journal of Priclinical Researc 2011,2:100-106.

.Harborne JB. Phytochemical methods to modern techniques of plant analysis. 3rd ed. New York, Chapman and Hall, 1998.

.Kokate, CK. Pharmacohnosy. 16th Ed. Mumbai, India,Nirali Prakashan, 2001.

.Lister E, Wilson P. Measurement of total phenolics and ABTS assay for antioxidant activity (personal communication), Crop Research Institute, Lincoln, New Zealand, 2001.

.Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity,LWT - Food Science and Technology 1995, 28(1): 25–30.

.Ruch R, Cheng S, and Klauning J. Prevention of cytoxicity and inhibition of intercellular communication antioxidant catechins isolated from Chinese green tea. Carcinogenesis 1989, 10:1003-1008.

.Gallagher AM, Flatt PR, Duffy G, Abdel-Wahab YHA. The effects of traditional antidiabetic plants on in vitro glucose diffusion, Nutr Res 2003, 23:413–424.

.Hansawasdi C, Kawabata J, Kasai T. α- amylase inhibitors from Roselle (Hibiscus sabdariffa Linn.) tea. Biosci Biotechnol Biochem 2000, 64:1041-43.

.Jung M, Park M, Lee HC, Kang YH, Kang ES, Kim SK. Antidiabetic Agents from Medicinal Plants. Current medicinal chemistry 2006, 13:1203-1218.

.Malathi V, Devi SS, Revathi K. Anti diabetic activity by the in vitro alpha amylase and alpha-glucosidase inhibitory activity of catharanthus roseus. The Bioscan, 2010, 5(4): 655-659.

.Yokozawa T, Nakagawa T. Inhibitory effects of Luobuma tea and its components against glucose-mediated protein damage. Food and Chemical Toxicology 2004, 42: 975-981.

.McLauglin, JL, Chan g CJ, Smith Dl. Simple bench - top bioassays (brine shrimp and potato discs) for the discovery of plant anti - tumour compounds. In: Kinghorn AD, Balandrin MF (editors).Human medicinal agents from plants. Washington: American Chemical Society 1993, 112 -137.

.Wadood A, Wadood N, Shah SA. “Effects of Acacia arabica and Carallumaedulis on blood glucose levels of normal and alloxan diabetic rabbits”. J. Pak. Med. Assoc 1989, 39(8): 208-212.

.Nammi S, Boini MK, Lodagala SD, Behara RB. The juice of fresh leaves of Catharanthusroseus Linn, reduces blood glucose in normal and alloxan diabetic rabbits. BMC Complement Altern Me 2003, 2: 3-4.

.Khan BA, Abraham A, Leelamma S. Hypoglycemic action of Murraya koenigii(curry leaf) and Brassica juncea (mustard) mechanism of action. Ind J BiochemBiophys 1995, 32:106-108.

.Rasool N, Rızwan K, Zubaır M, Naveed KR, Imranand Ahmed V. Antioxidant potential of different extracts and fractions of Catharanthus roseus shoots International Journal of Phytomedicine 2011,3:108-114.

.Bhutkar MA, Bhise SB, Comparison of Antioxidant Activity of Some Antidiabetic Plants, International Journal of Research in Pharmaceutical and Biomedical Sciences 2011, 2 (3):982-987.


  • There are currently no refbacks.

Copyright (c)

                AR Journals

Street 1st, Gaytri Vihar, Pinto Park, Gwalior, M.P. India

     (Design) 2009-2021


Follow @arjournals on Twitter