Reverse Phase Liquid Chromatography Coupled with Quadra pole- Time of Flight Mass Spectrometry for the Charecterisation of Phenolics from Acacia Catechu (L.f.) Willd.

Sulaiman C T, Gopalakrishnan V. K, Indira Balachandran

Abstract


A rapid method was developed for the identification of phenolics from Acacia Catechu .The analysis ofphenolic compounds was carried out by reversed-phase high-performance liquid chromatography (RPHPLC)coupled to electrospray ionization-time-of-flight mass spectrometry (ESI-Q-TOF-MS). Theaccurate mass data for the molecular ions were processed using the software Mass Hunter workstation (Agilent Technologies), which provided a list of possible elemental formulas by using theMolecular featured extraction editor. The subsequent structure characterization was carried out by atandem mass spectrometric method. Fragmentation behavior of phenolic compounds was investigatedusing ion trap mass spectrometry in negative mode. The total fragmentation of the compound ionleading to other fragments was corroborated by MS–MS. Five phenolic compounds have beenidentified from the methanolic extract of Acacia Catechu.


Keywords


Acacia, HPLC, LC-ESI-MS.

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