Effect Of Withania somnifera Leaf Extracts As Antibacterial Agent Against Multidrug Resistant Bacteria

Rinku Kumar Chandoria, Hotam Singh Chaudhary, Alka Yadav, Abhinav Gupta, Jay Prakash Yadav, Anju Rawat Shrivastav

Abstract


This study was designed to examine the phytochemical composition and to evaluate antibacterial potential of Withania Somnifera leaf against multidrug resistant bacteria & FT-IR Spectra of different bioactive compounds. Toluene, ethanol, and aqueous extracts of W. Somnifera (leaves) were subjected for in vitro antibacterial activity using agar well diffusion against different bacteria of clinical relevance including multi-drug resistant methicillin resistant Staphylococcus aureus (MRSA) and vancomycin resistant Enterococcus (VRE) sps.The toluene extract resulted maximal active as antibacterial agent and found significantly higher active than aqueous extract (P<0.05). The difference between antibacterial activities of toluene and ethanol or ethanol and aqueous extract was statistically non significant (P>0.05). The maximum activity was observed against Staphylococcus aureus. The extracts (at least one) also showed antibacterial activity against Bacillus cereus, coagulase negative staphylococcus, Streptococcus pneumoinae, Shigella dysenteriae, Methicillin resistant Staphylococcus aureus (MRSA) and vancomycin resistant Enterococcus (VRE) sps. Minimum inhibitory concentrations of plant extract were found in the ranges of 0.156mg/ml to 0.625mg/ml for test strains. Phytochemical analyses revealed the presence of various metabolites like phlobtannins, tannins, steroids and alkaloids which may contribute for the antimicrobial action of leaves extract of W. Somnifera. The partial characterization of the crude extracts by IR spectral analysis revealed the possible presence of different bioactive compounds in the extracts. The leaves of W. Somnifera showed promising antibacterial activity against various clinically important bacteria and multidrug resistant MRSA and VRE.

 


Keywords


Withania somnifera, Antibacterial activity, multi-drug resistance, IR spectral.

Full Text:

PDF

References


Copyright: WHO/P. Virot. The John E. Fogarty International Center NIH Publication 2008; Publication No. 08-6261:19.

Lowry FD. Staphylococcus aureus infections. N Engl J Med. 1998;20:520–32.

Austin DJ, Bonten MJ, Weinstein RA, Slaughter S, Anderson RM. Vancomycin-resistant Enterococci in intensive-care hospital settings: Transmission dynamics, persistence, and the impact of infection control programs. Proc Natl Acad Sci. 1999;96:6908-13.

Hiramatsu K, Hanaki H, Ino T. Methicillin-resistant Staphylococcus aureus clinical strain with reduced vancomycin susceptibility. J Antimicrob Chemother. 1997;40:135–6.

Engemann JJ, Carmeli Y, Cosgrove SE, Fowler VG, Bronstein MZ, Trivette SL, Briggs JP, et al. Adverse clinical and economic outcomes attributable to methicillin resistance among patients with Staphylococcus aureus surgical site infection. Clin Infect Dis. 2003;36:592-8.

Srivastava J, Lambert J, Vietmeyer N. Medicinal plants: An expanding role in development. World Bank Technical 1996; Paper. No. 320.

Chopra RN. Glossm of Indian Medicinal Plants. Council of Scientific and Industrial Research, Delhi;1956.

Suffness M, Douros J. Current status of the NCI plant and animal product program. J. Nat. Prod. 1982;45:1-14.

Chatterjee A, Pakrashi SC. The Treatise on Indian Medicinal Plants 1995; 4: 208-12.

Bone K. Clinical Applications of Ayurvedic and Chinese Herbs. Monographs for the Western Herbal Practitioner. Phytotherapy Press 1996;137-41.

Rai M, Acharya D, Singh A, Varma A. Positive growth responses of the medicinal plants Spilanthes calva and Withania somnifera to inoculation by Piriformospora indica in a field trial. Mycorrhiza 2001;11:123-8.

Murray PR, Baron EJ, Palled MA, Tenover FC, Yolken HR. Manual of Clinical Microbiology. ASM Press 1995;6:15-8.

Olurinola PF, Abuja, Nigeria. A laboratrical manual of pharmaceutical microbiology 1996;69-105.

Clinical and Laboratory Standard Institute, 940 West Valley Road, Suite 1400, Wayne Pennsylvania 19087-1898 USA. Performance standards for antimicrobial susceptibility testing. In Seventeenth Informational Supplement 2007; CLSI document M100-S17.

Martinez JL, Baquero F, Andersson DI. Predicting antibiotic resistance. Nat. Rev. Microbiol. 2007;5:958–65.

Cabello FC. Heavy use of prophylactic antibiotics in aquaculture: a growing problem for human and animal health and for the environment. Environ Microbiol. 2006;8:1137–44.

WHO. Cholera. Wkly. Epidemiol. Rec. 2010;85:293–308.

Samy RP, Ignacimuthu S. Antibacterial activity of some folklore medicinal plants used by tribals in Western Ghats of India. J Ethnopharmacol. 2000;69:63-71.


Refbacks

  • There are currently no refbacks.
';



Copyright (c)

                AR Journals

Street 1st, Gaytri Vihar, Pinto Park, Gwalior, M.P. India

              Copyright@arjournals.org (Design) 2009-2021

 

Follow @arjournals on Twitter