Phytochemical and clinical studies of the bioactive extract of Glycyrrhiza glabra L. Family Leguminosae

Nagwa Mohammed Ammar, Siham Salah El-Din El-Hawary, Amira Ahmed El-anssary, Nagwa Othman, Maha Galal, Ahmed Hamed El-Desoky



The aim of this study was the isolation, identification of the bioactive ingredients of Glycyrrhiza glabra L. Family Leguminosae, as well as the evaluation of their efficacy and ability to control plaque induced gingivitis in a preliminary clinical study. The dried powdered roots and rhizomes of G. glabra was subjected to successive extraction using organic solvents of increasing polarity. The total polar extract of the roots and rhizomes of G. glabra showed significant inhibition of carrageenan induced swelling of the hind rat paw(P>0.5).Seven phenolic compounds namely  liquiriteginin, liquiritin apioside, neoliquiritin apioside, isoliquiritin, isoliquritin apioside, licuraside2-(5-P-coumaryl apiosyl) and isoliquiritin were isolated from the total polar extract utilizing different chromatographic techniques (PC, TLC, CC, HPLC and LC/MS) and identified using spectral analysis (1H-NMR, 13C-NMR, 2D-NMR and ESI-MS).The effectiveness of the isolated bioactive fractions of G. glabra mouth rinse in the reduction of plaque and gingivitis was studied clinically, after the approval of its safety, and the results were significant.


Keywords: Glycyrrhiza glabra, anti-inflammatory activity, bioactive constituents, clinical trial, gingivitis, plaque index.

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.Fine DH, Furgang D, Sinatra K, Charles C, McGuire A, kumar LD. In vivo antimicrobial effectiveness of an essential oil contraining mouth rinse 12hours after a single use and 14 days use.JClin Periodontol.2005;32:335-40

.Blumenthal M, Goldberg A, Brinckmann J. Herbal Medicine: Expanded Commission E Monographs. American Botanical Council, Newton. 2000; 233–236.

.Ody P. The Complete Guide Medicinal Herbal. The Royal Horticultural Society. Dorling Kindersley Limited Great Britain.2000; 75.

.Nomura T, Fukai T, Akiyama T. Chemistry of phenolic compounds of licorice (Glycyrrhiza species) and their estrogenic and cytotoxic activities. Pure Appl Chem. 2002;74:1199–1206.

.Obolentseva GV, Litvinenko VI, Ammosov AS, Popova TP,Sampiev AM. Pharmacological and therapeutic properties of licorice preparations (a review). Pharm.Chem. J. 1999;33:24-31.

.Murav'ev IA, Semenchenko VF. Structure of triterpene saponins from the roots of Glycyrrhiza echinata. Chemistry of Natural Compounds. 1969; 5:13-14.

.Obukowicz MG, Welsch DJ, Salsgiver WJ, Martin-Berger CL, Chinn KS , Duffin KI, Raz A, Needle man P. Novel selective 6 or 5 fatty acid desaturase inhibitors as anti-inflammatory agents in mice. J. Pharmacol Exp. Ther. 1998; 287:157-66.

.Meng L, Mohan R, Kwok BH, Elofsson M, Sin N ,Crews CM. Epoxomicin, a potent and selective proteasome inhibitor,exhibits in vivo anti-inflammatory activity. Proc.Natl.Acad.Sci.USA. 1999;96:10403-8.

.Winter CA, Risley EA, Nuss GW. Carrageenan induced edema in hind paw of the rat as an assay for anti-inflammatory drugs. Proc. Soc. Exp. Biol. Med. 1962;111:544-547.

.Armitage P. Statistical methods in medical assays. 1st. Edn., Black-well Scientific Publications, London. 1971; 147.

.Loe H, Silness J. Periodontal disease in pregnancy. I. Prevalence and severity. Acta Odontol Scand. 1963; 21:533-51.

.British Pharmaceutical Codex.1973.

.Fu B, Li H, Wang X, Lee FS, Cui S. Isolation and identification of flavonoids in licorice and a study of their inhibitory effects on tyrosinase. J Agric Food Chem.2005; 53:7408-14.

.Hatano T, Takagi M, Ito H, Yoshida T. Acylated flavonoid glycosides and accompanying phenolics from licorice. Phytochemistry .1998; 47:287-293.

.Kase Y, Saitoh K, Ishige A, Komatsu Y. Mechanisms by which hange-shashin to reduce prostaglandin E2 levels. Biol Pharm Bull.1998; 21: 1277–81.

. Kassir ZA. Endoscopic controlled trial of four drug regimens in the treatment of chronic duodenal ulceration. Ir Med J.1985; 78:153–6.

.Aly AM, Al-Alousi L, Salem HA. Licorice: a possible anti-inflammatory and anti-ulcer drug. AAPS PharmSciTech.2005; 6:E74–82.

.Furuhashi I, Iwata S, Shibata S, Sato T, Inoue H. Inhibition by licochalcone A, a novel flavonoid isolated from liquorice root of IL-1β-induced PGE2 production in human skin fibroblasts. J Pharm Pharmacol.2005; 57:1661–6.

.Kang JS, Yoon YD, Cho IJ, Han MH, Lee CW, Park SK, Kim HM. Glabridin an isoflavan from licorice root, inhibits inducible nitric-oxide synthase expression and improves survival of mice in experimental model of septic shock. J Pharmacol Exp Ther.2005; 312:1187–94.



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