Antioxidant, Anti-inflammatory and Xanthine oxidase inhibitory Activity of Tephrosia purpurea L

Shivraj Hariram Nile, C N Khobragade, Se Won Park

Abstract


T. purpurea root extract was evaluated for antioxidant, anti-inflammatory, and xanthine oxidase (XO) inhibitory activities. Antioxidant activity was measured using ABTS and FRAP methods, anti-inflammatory activity was measured by Diene-conjugate and β-glucuronidase assay. In vitro XO inhibitory activity was measured by using cow milk xanthine oxidase enzyme. The average antioxidant activity of T. purpurea root extracts (1-2 µg/mL) in the reacting system revealed significant activity viz; 42.2 (ABTS) and 36.5 (FRAP) percent. The anti-inflammatory activities reveled, 45.40 and 70.50 percent inhibition. The result for XO inhibitory activity by plant extracts reveled, 95.5 % inhibition to that, off control (allopurinol) 92 % inhibition. The kinetic parameters of XO inhibition, revealed noncompetitive mode of inhibition, where, Km and Vmax of T. purpurea root extracts (25 to 100 µg/mL)) were,  0.25 mM/mL and 0.040, 0.036, 0.032 and 0.030 (µg/min) while for positive control Km and Vmax is 0.30 mM/mL and 0.045 (µg/min) respectively. Results suggest that, T. purpurea root can be exploited against diseases associated, with free radical formation and xanthine oxidase activity; further by isolation and structural elucidation of active phytochemicals from T. purpurea root.


Keywords


T. purpurea, Antioxidant, Anti-inflammatory, Xanthine oxidase

Full Text:

PDF

References


. Pavana P, Sethupathy S and Manoharan S. Protective role of T. purpurea ethanolic seed extract on glycoprotein components in streptozotocin induced diabetic rat. Int. J. Pharmacol. 2008; 4:114-119.

. Gopalakrishnan S, Vadivel E and Dhanalakshmi K. Phytochemical and pharmacognostical studies of Tephrosia purpurea L aerial and root parts. J. Herb. Med. Toxicol. 2009; 3: 3:73-78.

. Despande SS, Shah GB and Parmar NS. Antiulcer activity of Tephrosia purpurea in rats. Indian. J. Pharmacol. 2003; 35:168–172.

. Mohamed-Elamir F, Hegazy-Mohamed H, Abd El-Razek, Fumihiro Nagashima, Yoshinori Asakawa and Paul W. Rare prenylated flavonoids from Tephrosia purpurea. Phytochem. 2009; 70:1474–1477.

. Ames BN and Shigenaga M K. Oxidants is a major contributor to aging. Ann New York Acad Sci. 1992; 663:85-89.

. Esterbauer H, Striegl G, Puhl H and Rotheneder M. Continuous monitoring of in vitro oxidation of human low density lipoprotein. Free Rad. Res. Comm.1989; 6: 67-75.

. Bjelakovic G, Nikolova D, Gluud LL and Simonetti RG. Antioxidant supplements are used for prevention of several diseases. The. J. Am. Med. Assoc. 2007; 297:842-857.

. Sies H. Oxidative stress: oxidants and antioxidants. Exp Physiol.1997; 82:291-295.

. Vertuani S, Angusti A and Manfredini S. The antioxidants and pro-antioxidants network: an overview. Curr. Pharma. Des. 2004; 10:1677-1694.

. Mitchell RN and Cotran RS. Cell injury, adaptation, and death. In: Kumar V, Cotran RS, Robbins SL, Editors. Robbins, Basic Pathology, 7th ed. New Delhi: Harcourt (India) Pvt. Ltd. 2003; 2:3-33.

. Owen PL and Johns T. Xanthine oxidase inhibitory activity of Northeastern American plant remedies used for gout. J. Ethnopharmacol. 1999; 64: 149-160.

. Parks DA and Grarger DN. Xanthine oxidase: Biochemistry, distribution and physiology. Acta. Phy. Scand. 1986; 548:87-99.

. Khairul FK, Nurul HM and Zhari I. Antioxidative properties of various extract of Labisia pumila (Kacip Fatimah). Curr. Trends. Persp. 2005; 4:306-312.

. Beris H. Antioxidant affects a basis of drug selection. Drugs, 1991; 42:569-605.

. Muthuswamy U, Kuppusamy A, Arumugam S, Thirumalaisamy S, Varadharajan S and Thenvungal KR, Xanthine oxidase inhibitory activity of some Indian medical plants. J Ethnopharmacol. 2007; 109:547-551.

. Burda S and Oleszek W. Antioxidant and antiradical activities of flavonoids. J Agric Food Chem. 2001; 49:2774-2779.

. Hong Gao, Jun Nishida, Shizuka Saito and Jun Kawabata. Inhibitory Effects of 5, 6, 7-Trihydroxyflavones on Tyrosinase, Molecules. 2007; 12:86-97.

. Quist EH. Regulation of erythrocyte membrane shape by Ca2+. Biochem. Biophys. Res. Commun. 1980; 92:631-637.

. Dodge JF, Mitchell G and Hanahan DJ. The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes. Arch. Biochem. Biophys. 1963; 100:119-130.

. Nia R, Paper DH, Essien EE, Oladimeji OH, Iyadi KC and Franz G. Investigation into in-vitro radical scavenging and in-vivo anti-inflammatory potential of Tridax Procumbens. Niger. J. Physiol. Sci. 2003; 18:39-43.

. Khobragade CN, Bodade RG, Shinde MS, Jaju DR, Bhosale RB and Dawane BS. Microbial and xanthine dehydrogenase inhibitory activity of some flavones. J. Enz. Inhib. Med. Chem. 2008; 23:341-346.

. Kreamer BL, Siegel FL and Gourley GR. A novel inhibitor of beta-glucuronidase: L-aspartic acid. Ped Res. 2001; 4:460-466.

. Miller NJ, Johnston JD, Collis CS and Rice-Evans C. Serum total antioxidant activity after myocardial infarction. Annals. Clin. Biochem. 1997; 34:85-90.

. Baskar R, Lavanya R, Mayilvizhi S and Rajasekaran P. Free radical scavenging activity of antitumor polysaccharide fractions isolated from Ganoderma licidum (Fr.) P. Karst. Nat Prod Rad. 2008; 7:320-325.

. Huang D, Ou B and Prior L, The chemistry behind antioxidant capacity assays. J. Agric. Food. Chem. 2005; 53:1841–1856.

. Prior RL and Cao G. In vivo total antioxidant capacity: comparison of different analytical methods. Free. Rad. Biol. Med. 1999; 27:1173–1181.

. Buenger J, Ackermann H, Jentzsch A, Mehling A, Pfitzner I, Reiffen KA, Schroeder K R and Wollenweber U. An inter laboratory comparison of methods used to assess antioxidant potentials. Int. J. Cosmet. Sci. 2006; 28:135–146.

. Winrow VR, Winyard PG, Morris CJ and Blake DR. Free radicals in inflammation: second messengers and mediators of tissue destruction. Brit. Med. Bull. 1993, 49:506-522.

. Savill J and Haslett C. Granulocyte clearance by apoptosis in the resolution of inflammation. Sem.Cell. Bio. 1995; 6:385-393.

. Ito K, Kagaya H, Satoh I, Tsukamoto G and Nose T. The studies of the mechanism of anti-inflammatory action of 2-(5-ethylpyridin-2-yl) benzimidazole (KB-1043). Arzneimittelforschung. 1982; 2:117-122.

. Shewfelt RL and Purvis AC. Toward a comprehensive model for lipid peroxidation in plant tissue disorders. Hort. Sci. 1995; 30:213–218.


Refbacks

  • There are currently no refbacks.
';



Copyright (c)

                AR Journals

Street 1st, Gaytri Vihar, Pinto Park, Gwalior, M.P. India

              Copyright@arjournals.org (Design) 2009-2021

 

Follow @arjournals on Twitter