Phytochemical, Antioxidant and Cytotoxicity studies of Bambusa arundinacea leaves

Shaukat Hussain Naz, Muhammad Zubair, Komal Rizwan, Nasir Rasool, Muhammad Jamil, Muhammad Riaz, Muhammad Imran, Mazhar Abbas

Abstract


Medicinal plants, as a source of remedies, are widely used as alternative therapeutic utensil for the anticipation or treatment of many diseases. The Present study was carried out to investigate the medicinal importance of the plant Bambusa arundinacea leaves. The various extracts obtained from the plant leaves were analyzed for their phytochemical constituents, in vitro antioxidant, haemolytic acivities and stability of corn oil. The plant leaves contained appreciable levels of total phenolic contents (3.7-12.71 GAE mg/g of dry extract) and total flavonoid contents (2.31-6.71 CE mg/g of dry extract). DPPH radical scavenging IC50 and percentage inhibition of linoleic acid peroxidation was found to be in the range of 278-1536 μg/ml and 24.41-78.05% respectively. The antioxidant activity of plant extracts was also studied using corn oil as an oxidative substrate and found that it stabilized the oil.  The haemolytic effect of the plant leaves was found in range of 0.70-2.88%. The results of the present investigation demonstrated significant (p < 0.05) variations in the results. The correlation between the results of different antioxidant assays and oxidation parameters of oil indicated that leaves to be a potent source of antioxidants for stabilization of corn oil.

Keywords


Bambusa arundinacea, Antioxidants, Phytochemicals, Corn oil, Heamolytic activity.

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References


Ozer BC, Ozyoruk H and Çelebi SS and Yildiz A. Amperometric enzyme electrode for free cholesterol determination prepared with cholesterol oxidase immobilized in poly (vinylferrocenium) film. J. of enzyme microbio. Tech. 2007; 40: 262-265.

Vastano BC, Chen Y, Zhu N, Ho CT, Zhou Z and Rosen RT. Isolation and Identification of Stilbenes in Two Varieties of Polygonum cuspidatum . J. Agric. Food Chem, 2000; 48: 253-256.

Estuko K, Nobuyuki K and Hironobu, T. A comparative evaluation of in vitro antioxidant properties of bamboo bambusa arundinacea leaves extracts. jpn. soc. hortic. sci. 1998; 67: 805-881.

Jeong YH, Chung SY, Han AR, Sung MK, Jang DS, Lee J, Kwon Y, Lee HJ and Seo EK. P-glycoprotein inhibitory activity of two phenolic compound, (-)- syrigaresinol and tricin from Sasa borealis. Chem and Biodiver. 2007; 4: 12-16.

Shibata M, Yamatake Y, Sakamoto M, Kanamori M and Takagi K. Zasshi NY. Pharmacologcal studies on bamboo grass, acute toxicities, anti-inflammatory and anti-ulcerogenic activities of water soluble fraction (Folin) extracted from sasa albomarginata. Nihon Yakurigaku Zasshi. 1975; 71: 481-490.

Slinkard K and Singleton VL. Total phenol analyses automation and comparison with manual methods. Am. J. Enol. Viticult.1977; 28: 49-55.

Cai YZ, Sun M and Corke H. Antioxidant activity of betalains from plants of the Amaranthaceae. J. Agric. Food Chem. 2003; 51: 2288-2294.

Hou DX. tential mechanism of cancer chemoprevention by anthocyanin. Current Advan in Molec Med. 2003; 3: 149-159.

Trease GE, Evans WC. Pharmacognsy. 11th edn. Brailliar Tiridel Can. Macmillian publishers, 1989

Harborne JB. Phytochemical methods, London. Chapman and Hall, Ltd.1973; pp. 49-188.

Van-Burden TP, Robinson WC. Formation of complexes between protein and Tannin acid. J. Agric. Food Chem.1981; 1: 77.

Obdoni BO, Ochuko PO. Phytochemical studies and comparative efficacy of the crude extracts of some Homostatic plants in Edo and Delta States of Nigeria. Global J. Pure Appl. Sci.2001; 8 b: 203-208.

Boham BA, Kocipai AC. Flavonoids and condensed tannins from leaves of Hawaiian vaccinium vaticulatum and V. calycinium. Pacific Sci. 1974; 48: 458-463.

Rasool N, Rızwan K, Zubaır M, Naveed KR, Imran I and Ahmed VU. Antioxidant potential of different extracts and fractions of Catharanthus roseus shoots. Inter. J. of Phytomed. 2011; 3: 108-114.

Iqbal, S and Bhanger MI. Antioxidant properties and components of some commercially available varieties of rice bran in Pakistan. Food Chem. 2005; 93: 265-272.

Yen GC, Duh PD and Chuang DY. Antioxidant activity of anthraquinones and anthrone. Food Chem. 2000; 70: 307-315.

Mimica-Dukic N, Bozin B, Sokovic M, Mihajlovic B and Matavulj M. Antimicrobial and antioxidant activities of three Mentha species essential oils. Planta Medica. 2003; 69: 413-419.

American Oil Chemists Society (AOCS). Official and Recommended Practices of the American Oil Chemists Society, 5th ed., AOCS Press, Champaign IL, 1997. USA.

International Union of Pure and Applied Chemistry (IUPAC). Standard Methods for the Analysis of Oils, Fats and Derivatives, 7th revised and enlarged ed., edited by C. Paquot and A. Hautfenne, Blackwell Scientific, 1987. London..

Powell WA, Catranis CM and Maynarrd CA. Design of self processing antibacterial peptide of plant protection. Letters in app microbio. 2000; 31: 163-165.

Zou Y, Lu Y and Wei D. Antioxidant activity of a flavonoid rich extract of Hypericum perforatum L. in vitro. J. of Agri. and Food Chem. 2004; 52: 5032-5039.

Frega N, Mozzon M and Lercker G. Effect of free fatty acids on the oxidative stability of vegetable oils. J. of Am. Oil Chem. Soc.1999; 76: 325-329.

Fishwick MJ and Swoboda PAT. Measurement of oxidation of polyunsaturated fatty acids by spectrophotometric assay of conjugated derivatives. J.of Sci Food and Agri.1977; 28: 387–391.

Sharma P and Sharma JD. In vitro hemolysis of human erythrocytes by plant extracts with antiplasmodial activity. J. of ethnopharmaco. 2001; 74: 239-243.


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