Antimicrobial activity of lemongrass and oregano essential oil against standard antibiotic resistant Staphylococcus aureus and field isolates from chronic mastitis cow

Jae-Young Choi, Dereje Damte, Seung-Jin Lee, Jong-Choon Kim, Seung-Chun Park


The study was carried out to investigate the antibacterial activity of lemongrass and Oregano essential oil against standard antibiotic resistant Staphylococcus aureus strains and field isolates from a chronic mastitic cow. Disc diffusion and minimum inhibitory concentration (MIC) assay were used to investigate antibacterial activity of lemongrass and Oregano essential oil while their combined effect with antibiotic were assessed using a microbroth chequerboard technique. For identification of the essential oil component Gas Chromatography Coupled Mass Spectroscopy (GC/MS) analysis was performed. Disc diffusion assay revealed dose-dependent inhibition zone with lemongrass oil showing consistently larger inhibition zone than oregano oil. Lemongrass essential oils were further evaluated for its antibacterial activity in combination with antibiotic which resulted both synergistic and additive interaction. Lemongrass oil-amoxicillin combination showed synergistic interactions against all tested organism while the combination for lemongrass oil-norfloxacin showed synergistic effect for two field isolates and an additive for the remaining strains. The current study indicated that both lemongrass and oregano essential oils exhibit antibacterial activity, and lemongrass oil in combination with antibiotics could be a potential agent for antibacterial treatments against antibiotic resistant bacterial infections.


Lemongrass; Oregano essential oil, Resistant, S. aureus, antibiotics

Full Text:




. Helander I. K., Alakomi H. L., Latva-Kala K., Mattila-Sandholm T., Pol I., Smid E. J., von Wright A. (1998) Characterization of the action of selected essential oil components on Gram negative bacteria. J Agric Chem. 46: 3590–3595.

. Chorianopoulos N., Kalpoutzakis E., Aligiannis N., Mitaku S., Nychas G. J., Haroutounian S. A. (2004) Essential oils of Satureja, Origanum, and Thymus species: chemical composition and antibacterial activities against foodborne pathogens. J Agric Food Chem. 52: 8261-7.

. Si H., Hu J., Liu Z., Zeng Z. L. (2008) Antibacterial effect of oregano essential oil alone and in combination with antibiotics against extended-spectrum b-lactamase-producing Escherichia coli. FEMS Immunol Med Microbiol. 53: 190-4.

. Melter O., Aires de Sousa M., Urbaskova P., Jakubu V., Zemlickova H., de Lencastre H. (2003) Update on the major clonal types of methicillin-resistant Staphylococcus aureus in the Czech Republic. J Clin Microbiol. 41: 4998-5005.

. Lee J. H. (2003) Methicillin (Oxacillin)-resistant Staphylococcus aureus strains isolated from major food animals and their potential transmission to humans. Appl Environ Microbiol. 69: 6489-94.

. Kobayashi H. (2005) National hospital infection surveillance on methicillin-resistant Staphylococcus aureus. J Hosp Infect. 60: 172-5.

. Tenover F. C., Biddle J. W., Lancaster M. V. (2001) Increasing resistance to vancomycin and other glycopeptides in Staphylococcus aureus. Emerg Infect dis. 7: 327–32.

. Barnard F. M., Maxwell A. (2001) Interaction between DNA gyrase and quinolones: effects of alanine mutations at GyrA subunit residues Ser(83) and Asp(87). Antimicrob Agents Chemother. 45(7): 1994-2000.

. Ouattara B., Simard R. E., Holley R. A., Piette G. J., Bégin A. (1997) Antibacterial activity of selected fatty acids and essential oils against six meat spoilage organisms. Int J Food Microbiol. 37: 155-62.

. Prabuseenivasan S., Jayakumar M., Ignacimuthu S. (2006) In vitro antibacterial activity of some plant essential oils. BMC Complement Altern Med. 6: 39.

. V´agi E., Sim´andi B., Suhajda A., H´ethelyi E. (2005) Essential oil composition and antimicrobial activity of Origanum majorana L. extracts obtained with ethyl alcohol and supercritical carbon dioxide. Food Res Int. 38: 51–57.

. Tohidpour A., Sattari M., Omidbaigi R., Yadegar A., Nazemi, J. (2010) Antibacterial effect of essential oils from two medicinal plants against Methicillin-resistant Staphylococcus aureus (MRSA). Phytomedicine. 17: 142-5.

. Jayaraman P., Sakharkar M. K., Lim C. S., Tang T. H., Sakharkar K. R. (2010) Activity and interactions of antibiotic and phytochemical combinations against Pseudomonas aeruginosa in vitro. Int J Biol Sci. 6: 556-68.

. Burt S. A. (2004) Essential oils: their antibacterial properties and potential applications in foods: a review. Inter J Food Microbiol. 94: 223-253.


  • There are currently no refbacks.

Copyright (c)

                AR Journals

Street 1st, Gaytri Vihar, Pinto Park, Gwalior, M.P. India

     (Design) 2009-2021


Follow @arjournals on Twitter