Antidiabetic effect of various fractions of Habenaria plantaginea root in streptozotocine-induced diabetic rats

Debajyoti Das, Goutam Ghosh, Agnimitra Dinda, Paidisethy Sudhir Kumar


The aim of this study is to evaluate the antidiabetic effect of methanolic extract of Habenaria plantaginea and its various fractions in different animal models. The effect of repeated oral administration of methanolic extract along with its n-hexane, ethyl acetate and n-butanol fractions on serum lipid profile and plasma enzyme levels in diabetic rats was also examined. The effect was found to be pronounced in n-butanol fraction. In oral glucose tolerance test, reduction of fasting blood glucose levels took place from 60 min on administration of methanolic extract and its various fractions. The n-butanol fraction has almost similar effect as that of standard drug Glibenclamide (10 mg/kg b. w). After 15 days of treatment, n-butanol fraction showed maximum reduction of blood glucose levels (64.28 %).  Total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL) and very low density lipoprotein (VLDL) levels were found to be decreased by 38.98, 28.06, 56.26 and 37.77% respectively in diabetic rats whereas, cardioprotective, high density lipoprotein (HDL) was increased by 31.65%. The n-butanol fraction also restored the altered plasma enzyme such as serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT) and alkaline phosphatase (ALP) levels to near normal. These results clearly indicate that n-butanol fraction of methanolic extract of Habenaria plantaginea possess high antidiabetic potential along with significant hypoglycaemic and hypolipidemic effects.


Habenaria plantaginea, antidiabetic, streptozotocin

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. Chandra A, Singh RK, Tewari L. Antioxidative potential of herbal hypoglycemic agents in diabetes-an overview. SFRR-Indian Bulletin 2004; 3: 24–26.

. Kesari AN, Kesar S, Singh SK, Gupta RK, Watal G. Studies on the glycemic and lipidemic effect of Murraya koenigii in experimental animals. J Ethnopharmacol. 2007; 112: 305–311.

. Day C. Traditional plant treatment for diabetes mellitus: pharmaceutical foods. Britain J Nutri. 1998; 80: 5–6.

. Boyle JP, Honneycutt AA, Narayan KM, Hoerger TJ, Geiss LS, Chen H, Thompson TJ. Projections of diabetes burden through 2050: impact of changing demography and disease prevalence in the US. Diabetes Care 2001; 24: 1936–1940.

. Zhang BB, Moller DE. News approaches in the treatment of type 2 diabetes. Current Opinion of Chemical Biology 2000; 4: 461–467.

. Dhanbal SP. Evaluation of therapeutic activity and development of quality control profiles for some antidiabetic herbal drugs. Indian J Pharm Educ. 2004; 8:163–165.

. Nadkarni KM, Nadkarni AK. The Indian Materia Medica. Mumbai, India: Popular Prakashan (Pvt.) Ltd.; 1996. p. 964-65. (vol 1)

. Maridass M, Zahir Hussain MI, Raju G. Phytochemical Survey of Orchids in the Tirunelveli Hills of South India. Ethnobotanical Leaflets 2008; 12: 705-712.

. Mohammad HM. Therapeutic Orchids: Traditional uses and recent advances – An Overview. Fitoterapia 2011; 82:102-140.

. Harborne JB. Phytochemical Methods. London: Chapman & Hall; 1998. p.60.

. Ghosh MN. Fundamental of Experimental Pharmacology. 3rd ed. Kolkata: Hilton and Company; 2005. p.197.

. Theodorou NA, Vrbova H, Tyhurst M, Howell SL. Management of intestinal amoebiasis by an indigenous drug Kantaki karanja (Caesalpinia crista L.). Diabetol. 1980; 18: 313-318.

. Andallu B, Varadacharyulu NC. Control of hyperglycemia and retardation of cataract by mulberry (Morus indica L.) leaves in streptozotocin diabetic rats. Indian J Exp Biol. 2002; 40: 791-795.

.Kesari AN, Gupta RK, Singh SK, Diwakar S, Watal G. Hypoglycemic and antihyperglycemic activity of Aegle marmelos seed extract in normal and diabetic rats. J Ethnopharmacol. 2006; 107: 374–379.

. Shirwaikar A, Rajendran K, Barik, R. Effect of aqueous bark extract of Garuga pinnata Roxb. in streptozotocin–nicotinamide induced type-II diabetes mellitus. J Ethnopharmacol. 2006; 107: 285–290.

. Burkhardt RT, Batsakis JC. An interlaboratory comparison of serum total protein analysis. Am J Clin Pathol. 1978; 70: 508–510.

. Pierre NM, Demaekerl A, Marja H, Helga TD, Henk B. Precipitation methods for high density lipo-protein cholesterol measurement compared, and final evaluation under routine operating conditions of a method with a low sample-to-reagent ratio. Clin Chem. 1997; 43: 663–668.

. MeGown MW, Artiss JD, Strandberg DR, Zak B. A peroxidase-coupled method for the colorimetric determination of serum triglycerides. Clin Chem. 1983; 29: 538–542.

. Kind PRN, King EJ. Estimation of plasma phosphatase by determination of hydrolysed phenol with antipyrin. J Clin Pathol.1954; 7: 322-331.

. Bailey CJ, Day C. Traditional plant medicines as treatments for diabetes. Diabetes Care 1989;12: 553-564.

. Trivedi NA, Mazumdar B, Bhatt JD, Hemavathi KG. Effect of Shilajit on blood glucose and lipid profile in alloxan induced diabetic rats. Indian J Pharmacol. 2004; 36:373-376.

. Hardy KJ, McNutty SJ. Oral hypoglycemic agents. Med Digest 1997; 23: 5-9.

. Badole S, Patel N, Bodhankar S, Jain B, Bhardwaj S. Antihyperglycemic activity of aqueous extract of leaves of Cocculus hirsutus (L.) Diels in alloxan-induced diabetic mice. Indian J Pharmacol. 2006; 38:49-53.

. Jacot E, Assal JPH. Regulation de la glycemie. In: Pharmacologiedes Concepts Fondamentaux aux Applications Therapeutiques, editor Schorderet. 1989. p. 481-494.

. Marles RJ, Farnsworth NR. Antidiabetic plants and their active constituents. Phytomedicine 1995; 2: 137–189.

. Ali L, Azad Khan AK, Mamun MIR, Mosihuzzaman M, Nahar N, Alan MNE, Rokeya B. Studies on the hypoglycemic effects of fruits pulp, seed and whole plant of Momordica charantia on normaland diabetic model rats. Planta Med. 1993; 59: 408–412.

. Kamanyi A, Dajmen D, Nkeh B. Hypoglycemic properties of the aqueous root extract of Morinda lucida (Rubiacea) study in the mouse. Phytother Res. 1994; 8: 369–371.

. Foreston WC, Tedesco FJ, Starnes EC. Marked elevation of serum transaminase activity associated with extrahepatic biliary tract disease. J Clin Gastroenterol. 1985; 76: 502–505.

. Hultcrantz R, Glaumann H, Lindberg G. Liver investigation in 149 asymptomatic patients with moderately elevated activities of serum aminotransferases. J Gastroenterol. 1996; 21: 109–113.

. Ohaeri OC. Effect of garlic oil on the levels of various enzymes in the serum and tissue of streptozotocin diabetic rats. Bio sci Rep. 2001; 21:19–24.

. Navarro CM, Montilla PM, Martin A, Jimenez J, Utrilla PM. Free radicals scavenger and antihepatotoxic activity of Rosmarinus. Planta Med. 1993; 59: 312–314.

. Almdal TP, Vilstrup H. Strict insulin treatment normalizes the organic nitrogen contents and the capacity of urea–N synthesis in experimental diabetes in rats. Diabetologia 1988; 31: 114–118.

. Taskinen MR. Lipoprotein and apoproteins in diabetes, in Current Topics in Diabetes Research, editors. Belfiore F, Bergnan RN, Molinatt GM, Informa Health Care. 1993. p. 122–134.

. Wilson PWF. High density lipoprotein, low density lipoprotein and coronary heart disease. American J Cardiol. 1990; 66: 7A– 10A.


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