Tanvi Chincholkar, Tahir M Malla, Narayanan Ganesh


In the present paper, we provide an insight of the radioprotective potential of Adhatoda vasica leaf extract. Mice bone marrow cells were used as the test system. Animals were fed with 500mg/kg body wt. and 1000mg/kg body wt. of ethanolic extract of the plant prior to 9 Gray γ-radiation exposure and chromosomal aberration assay and micronucleus assay were performed. The frequency of chromosomal aberrations and micronuclei among different treatment groups and the controls was compared. The results revealed a significant decrease in the frequency of chromosomal aberrations as well as the micronucleus possessing cells in the groups pre-treated with A. vasica extract as compared to the untreated group. Better radioprotection was observed in 1000mg/kg-body wt. dosage group as compared to 500mg/kg-body-wt. dosage group. These findings were suggestive of radioprotective potential of A. vasica leaf extract.


Radioprotection, Adhatoda vasica, Chromosomal aberrations, Micronucleus

Full Text:



Delaney G et al. (2005). The role of radiotherapy in cancer treatment: Estimating optimal utilization from a review of evidence-based clinical guidelines. Cancer, 104:1129–1137.

The Swedish Council on Technology Assessment in Health Care (SBU). Systematic Overview of Radiotherapy for Cancer including a Prospective Survey of Radiotherapy Practice in Sweden 2001 – Summary and Conclusions. Acta Oncologica, 2003, 42: 357–365.

Uma Devi P, Rao BSS, Nagarathnam A. Introduction to radiation biology. BI Livingstone Pvt. Ltd. (2000) 5:65

Emerit I., Oganesian N., Sarkisian T., Arutuyunyan R., Pogosian A., Asrian K., Levy A., and Cernjavski L. (1995) Radiation-induced clastogen factors: Anticlastogenic effects of Gingko biloba. Free Radic. Biol. Med., 18: 985-991.

Ganasoundari A., Uma Devi P., and Roa M. N. A. (1997) Protection against radiation-indused chromosome damage in mouse bone marrow by Ocimum sanctum. Mutat. Res, 373: 271-279.

Kumar M., Sharma M. K., Saxena P. S., and Kumar A. (2003) Radioprotective effect of Panax ginseng on the phosphatases and lipid peroxidation level in testes of Swiss albino mice. Biol. Pharm. Bull, 26: 308-312.

Goel H. C., Prasad J., Singh S., Sagar R. K., Agrawala P. K., Bala M., Sinha A. K. and Dogra R. (2004) Radioprotective potential of herbal extract of Tinospora cordifolia. J. Radiat. Res. 45: 61-68.

Singh I., Sharma A., Nunia V. and Goyal P. K. (2005) Radioprotection of Swiss albino mice by Emblica officinalis. Phytother. Res. 19: 444-446.

Sunila E. S. and Kuttan G. (2005) Protective effect of Piper longum fruit ethanolic extract on radiation-induced damages in mice: A preliminary study. Fitoterapia. 76: 649-655.

Samarth R. M. and Goyal A. (2003) Mentha piperita (Linn.) leaf extract provides protection against radiation indeuced chromosomal damage in bone marrow of mice. Indian J. Exp. Biol. 41: 229-237.

Jagetia G. C., Baliga M. S., Venkatesh P. and Ulloor J. N. (2003) Influence of ginger rizhome (Zingiber officinale Rosc) on survival, glutathione and lipid peroxidation in mice after whole-body exposure to gamma radiation. Radiat. Res. 160: 584-592.

Wealth of India, 1989. Raw Materials. Council Sci. Ind. Res., New Delhi, India.

Dorsch, W. and Wagner, H. (1991) New antiasthmatic drugs from traditional medicine. Int. Arch. Allergy Appl. Immunol. 94: 262–265.

Nath, D., Sethi, N., Singh, R.K., and Jain, A.K., (1992) Commonly used Indian abortifacient plants with special reference to their teratologic effects in rats. J. Ethnopharmacol. 36: 147–154.

Grange, J.M. and Snell, N.J., (1996) Activity of bromhexine and ambroxol, semisynthetic Emertuberculosis in vitro. J. Ethnopharmacol. 50: 49–53.

Kumar Meenal, et al, (2007). Protective Effect of Adhatoda vascia Nees Against Radiation-Induced Damage at Cellular, Biochemical and Chromosomal Levels in Swiss Albino Mice. eCAM 4(3)343–350.

Preston R. J., Dean B. J., Galloway A. F., Mcfee Sheldy (1987) Mammalian in vivo cytogenic assay analysis of chromosomal aberration in bone marrow cells. Mutat Res. 189: 157-165.

Seabright, M. (1971). A rapid banding technique for human chromosomes. Lancet 2: 971-972.

Kumar A., Ram J., Samarth R. M., and Kumar M. (2005) Modulatory influence of Adhatoda vasica Nees leaf extract against gamma irradiation in Swiss albino mice. Phytomedicine 12:285–293

Hall, E.J., (1978). In: Radiobiology for the Radiologists, second ed. Harper & Row Publishers, Philadelphia.

Biaglow, J.E., Varnes, M.E., Epp, E.R., Clark, E.P., (1987) In: Cerrutti, P.A., Nygaard, O.F., Simic, M.G. (Eds.), Anticarcinogenesis and Radiation Protection, vol. 387. Plennum Press, New York.


  • There are currently no refbacks.

Copyright (c)

                AR Journals

Street 1st, Gaytri Vihar, Pinto Park, Gwalior, M.P. India

     (Design) 2009-2021


Follow @arjournals on Twitter