Preliminary Analysis of Two Medicinal Plants against causative organism of Bovine Mastitis

gopi nath, Suneetha T. B, Mruganka Deshpande




Bovine Mastitis is an intramammary infection which is most common among the dairy cattle and continues to be the most costly disease to the dairy farmers. Presently, antibiotics are used for treatment of mastitis leading to the development of antibiotic resistant strains and consumer health problem. The present study investigates in vitro antibacterial activity of two medicinal plants namely Cymbopogon citratus and Punica granatum L against causative organisms of bovine mastitis. Aqueous and methanol extracts of two plants were investigated by agar well-diffusion method. Methanol and aqueous extracts of Cymbopogon citrates and Punica granatum L.  were found to possess potency against infectious pathogens Staphylococcus aureus, Coagulase negative Staphylococcus aureus, Streptococcus uberis and Escherichia coli isolated locally from clinical and sub clinical samples of Mastitis from Karnataka region whereas .Phytochemical screening of the plants revealed mainly the presence of alkaloids, tannin, saponin, terpenoids and falvonoids.

Keywords: Cymbopogon citratus, Punica granatum L, Aqueous extracts, methanol extracts, phytochemical screening, antibacterial activity


Keywords: Cymbopogon citratus, Punica granatum L, Aqueous extracts, methanol extracts, phytochemical screening, antibacterial activity

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Adaobi Ezike, Godwin Ebi, Peter Akah, Uchechukwu Okeudo. 2011. J. Chem. Pharm. Res.,

, 3(3):676-679

Annapoorani Chockalingam, Dante S. Zarlenga, Douglas D. Bannerman., American Journal

of Veterinary Research. 68 (11): 2007, pp: 1151 –59.

Bauer, A. W., Kirby, W.M.M., Sherris, J. C., Turck, M., 1966. Am. J. Clin. Pathol. 45: 493-

C.K. Hindumathy., 2011. World Academy of Science, Engineering and Technology; 193-197

D. H. Tambekar and S. B. Dahikar., 2011 J. Chem. Pharm. Res., 2010, 2(5): 494-501

Jamine. R,Daisy. P and Selvekumar.B.N., 2007. Research Journal of Microbiology.2


Klastrup O. Dairy Federation. 85: 1975, pp: 49-52.

Kubmarawa D, Khan ME, Punah AM and Hassan 2008. Journal of Medicinal plants

research 2(12):352-355.

Parekh, J., Chanda, S., 2007b. Afr. J.Biol. Res. 10: 175-181

Pranay Jain, Gulhina Nafis; Journal of Pharmacy Research 2011, 4(1), 128-129.

Prasad, N.R., Viswanathan.S., Renuka Devi, J., Vijayashree Nayak., Sweth,V.C., Archana

parathasarathy, N and Johana Rajkumar., 2008. Journal of Medicinal Plants Research.2:268-

Sachin Kumar, Hotam Singh Choudhary, Chandrabhan Seniya., 2011. J. Chem. Pharm.

Res., 2011, 3(4):854-860

Sharma B and Kumar P. 2009. International journal of applied research in natural

products,1(4); 5-12

Sofowora, A., 1993. Recent trends in research into African medicinal plants.

J.Ethnopharmacol. 38: 209-214.

Trease, G.S. and Evans, H.C., 1978. Textbook of pharmacognosy. 9th edition. Bailiar Zindall

and Co., London.

Wynn GS.2001.Herbs in Veterinary Medicine. Alternative Veterinary Medicine.


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