Antioxidant Activity of Various Extracts and Organic Fractions of Ziziphus jujuba

nasir rasool, Komal Rızwan, Muhammad Zubaır, Tahsin Gulzar, Iftikhar Hussain Bukhari, Mazhar Amjad Gilani, Adeela Amjad


Antioxidant effectiveness of indigenous medicinal plant Ziziphus jujuba shoots extracts and fractions with different polarity solvents (n–hexane, ethylacetate, methanol, chloroform) was assessed for total phenolics content (TPC), total flavonoid contents (TFC), DPPH radical scavenging activity and percentage inhibition of peroxidation in linoleic acid system. The shoots extracts and fractions contained appreciable levels of total phenolic contents 310-823 GAE (mg/100g Dry plant matter) and total flavonoid contents 210-650 CE (mg/100g of Dry plant matter). The Ziziphus jujuba extracts and various organic fractions also exhibited good DPPH 50% inhibition (IC50) ranges from 23.1µg/mL to 52.5 µg/ml and Inhibition of Peroxidation in Linoleic Acid  20.1 to 70.1%., respectively. Of the Ziziphus jujuba shoots extracts and fractions tested, 100% methanolic extract and 100% chloroform fraction exhibited the maximum antioxidant activity, the results of the present investigation demonstrated significant (p< 0.05) variations in the antioxidant activity. The results of the present comprehensive analysis demonstrated that Ziziphus jujuba shoots extracts and organic fractions are a viable source of natural antioxidants and might be exploited for functional foods and nutraceutical applications


Total phenolic contents; flavonoids; antioxidant activity; DPPH free radical scavenging; Ziziphus jujuba.

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Mukhtar HM, Ansari SH, Ali M and Naved T. New compounds from Zizyphus vulgaris.

Pharmaceu. Biology. 2004; 42: 508-511.

Meena SK, Gupta NK, Gupta S, Khandelwal SK and Sastry EVD. Effect of sodium chloride on the growth and gas exchange of Young Ziziphus seedling rootstocks. J. Horti. Sci. and Biotechnol. 2003; 78: 454-457.

Ghannadi A, Naser AT, Ardestani M and Mozhgan. Volatile constituents of the leaves of Ziziphus spinachristi L. From Bushehr, Iran. J. essential oil research. 2003; 16: 412-415.

Zhao J, Li SP, Yang FQ, Li P and Wang YT. Simultaneous determination of saponins and fatty acids in Ziziphus jujuba (Suanzaoren) by high performance liquid chromatography- vaporative light scattering detection and pressurized liquid extraction. J. Chromatography A. 2006; 110: 188-194.

Li JW, Ding SD and Ding XL. Comparison of antioxidant capacities of extracts from five cultivars of Chinese jujube. Process Biochem. 2005; 40: 3607-3613.

Chaovanalikit A and Wrolstad RE. Total anthocyanins and total phenolics of fresh and processed cherries and their antioxidant properties. Food chem. And toxicol. 2004; 69: 67-72.

Dewanto V, Wu X, Adom KK and Liu RH. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J. of Agri and Food Chem. 2002; 50: 3010–3014.

Tepe B, Sokmen A, Daferera D, Soknen M and Polissiou M. Antimicrobial and antioxidant

activities of the essential oil and various extracts of Salvia tomentosa Miller (Lamiaceae). Food

Chem. 2005; 90: 333-340.

Gulcin I. Antioxidant activity of L-adrenaline: A structure activity insight. Chemico-Biological

Interactions,2009; 179: 71-80.

Yen GC, Duh PD and Chuang DY. Antioxidant activity of anthraquinones and anthrone. Food Chem. 2000; 70: 307-315.

Siddhuraju P and Becker K. Antioxidant properties of varion extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera) leaves. J. Agri. And Food Chem. 2003; 51: 2144-2155.

Tung YT, Wu JH, Kuo YH and Chang ST. Antioxidant activities of natural phenolic compounds from Acacia confusa bark. Bioresource Technol. 2007; 98: 1120-1123.


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