Potential antioxidant and hypoglycaemic effect of the flower extract of Bougainvillea spectabilis Willd. (Nyctaginaceae)

Fave Yohanna Tata, Hafsat Ali Sa’ab, Abdulkadir Bukar Bababe, Muhammad Mustapha Hassan, Wazis Chama Haruna, Cletus Anes Ukwubile

DOI: http://dx.doi.org/10.5138/09750185.2460


The study evaluated the antioxidant and hypoglycaemic potentials of the ethanol flower extract of Bougainvillea spectabilis. Flower extract of Bougainvillea spectabilis were obtained by cold maceration method. The preliminary phytochemical screening and hypoglycaemic effect were carried out using standard methods. Antioxidant screening was carried out using DPPH antioxidant assay test. Five rats were used for each group; normal control, standard control, 100, 200 and 400 mg/kg b.w. doses of the crude ethanol flower extract of B. spectabilis to test the hypoglycaemic effect in alloxan-induced diabetic rats. Blood glucose levels were measured at different time intervals. The preliminary phytochemical screening revealed the presence of alkaloids, saponins, strerols, terpenoids, flavonoids, and carbohydrates. The crude ethanol fraction demonstrated least antioxidant activity while the chloroform fraction showed the highest antioxidant activity followed by the hexane fraction. The ethanol flower extract showed a significant dose dependent hypoglyceamic effect after 24 hours of administration.  Of the doses tested, highest hypoglycaemic effect was observed by the ethanol flower extract of the dose 400 mg/kg at 24 hours. The findings revealed that non-polar fractions have more antioxidant activity than the polar fractions while the ethanol flower extract of Bougainvillea spectabilis possesses a delayed hypoglycaemic effect.


Alloxan, antioxidant, Bougainvillea spectabilis, DPPH, hyperglycaemia


Narhe, S., Kshirsagar, S. S., Patil V. S., 2018. Review on Medicinal Herbs Used for Diabetes, International Journal of Pharmaceutical and Clinical Research, 10 (8), 224-228 [2] Karthikeyan, J., Kavitha, V., Abirami, T., Bavani, G., 2017. Medicinal plants and diabetes mellitus, Journal of Pharmacognosy and Phytochemistry: A review 6(4), 1270-1279 [3] Orasanu, G., Plutzky, J., 2009. The pathologic continuum of diabetic vascular disease. J Am Coll Crdiol; 53(5), 35-42. [4] Junejo, J. A., Gogoi, G., Islam, J., Rudrapa, M., Mondal, P., Hazarika, H., Zaman, K., 2018. Exploration of antioxidant, antidiabetic and hepatoprotective activity of Diplazium esculentum - A wild edible plant from North Eastern India, Future Journal of Pharmaceutical Sciences 4, 93-101 [5] Barbosa, A., Silveira, G.D., de Menezes, I., Neto J., Bitencurt, J., Estavam C.D., de Lima, A., Thomazzi, S.M., Guimaraes, A.G., Quintans, L.J., 2013. Antidiabetic effect of the Chrysobalanus icaco L. aqueous extract in rats. J. Med. Food. 16, 538–543. [6] Junejo, J.A., Singh, K.H., Islam, J., Mandal, P., Zaman, K., 2016. Phytochemical analysis and in vivo toxicity evaluation of green vegetable Tetrastigma angustifolia (Roxb.) leaves, Asian J Biol. Life Sci. 5, 1-7. [7] Yu-Jie, Z., Ren-You G., Sha, L., Yue, Z., An-Na, L., Dong-Ping X., Hua-Bin, L., 2015. Antioxidant Phytochemicals for the Prevention and Treatment of Chronic Diseases, Molecules 20(12), 21138–21156. [8] International Diabetes Federation, 2015. Diabetes Atlas, 7th ed., International Diabetes Federation, Brussels. [9] World Health Organization., 2016. Prevalence of diabetes in some African countries. [10] Sharif, M. S., Ohidul, I., Azad, A.K., Mustafezur, R M., Khorshed A. A., Khairuzzaman, M., Jannatun F., Manirul I., 2018. Phytochemical Profiling and Evaluation of Antioxidant and Antidiabetic Activity of Methanol Extract of Spinach (Spinacia Oleracea L.) leaves. Int. J. Pharm. Sci. & Scient. Res. 3(8), 24-27 [11] Shaw, J.E., Sicree, R.A., Zimmet, P.Z., 2010. Global estimates for the prevalence of diabetes for 2010 and 2030. Diabetes Research and Clinical Practice. [12] Zimmet, P., Alberti, K.G.M.M., Shaw, J., 2001. Global and Societal Implications of the Diabetes Epidemic. Nature 414(13), 782-787. [13] Ali, N., Shah, S.W.A., Khan J., Rehman, S., Imran, M., Hussian, I., Shehbaz, N., Jamshed, H and Khan, S., 2010. Pharmacotherapy-Based Problems in the Management of Diabetes Mellitus. J Young Pharm. 2(3), 311-314. [14] Calixto, J. B., 2005. Twenty-five years of research on medicinal plants in Latin America: a personal view. Journal of Ethnopharmacology. 100(1-2), 131–134. [15] World Health Organization., 2002. Traditional Medicine Strategy World Health Organization, Geneva. [16] Malviya, N., Jain, S., Malviya, S., 2010. antidiabetic potential of medicinal plants, Journal pharmaceutical society, 67(2), 113-118. [17] Jawla, S., Kumar, Y., Khan, M.S.Y., 2012. Hypoglycemia activity of Bougainvillea spectabilis stem bark in normal and alloxan induced diabetic rats. Asian Pac J Trop Biomed; 2, 919–23. [18] Evans, W.C., Trease and Evans., 2002. Pharmacognosy 15th ed., W.B Saunders, Edinburgh, pp585. [19] Sofowora, E.A., 2008. Medicinal and Traditional Meidicine in Africa, (3rd edition). Spectrum book Ltd Ibadan, Nigeria. 89-133. [20] Deora, PS., Mishra, CK., Mavani, P., Asha, R., Shirivastava, B., and Rajesh, K. N. 2010. Effective alternative methods of LD50 help to save number of experimental animals. J. chem. Pharm. Res; 2(6): 450-453 [21] Krishna, RK., Kumari, SK., Vinodha, M., Sebastin, A., and Gomathi, R. 2012. in vitro free radical scarvenging activity of amorphophallus commutatus an endemic aroid of western gharts, south india. international research journal of pharmacy; l3(2): 133. [23] Cheng, D.M., Pogrebnyak, N., Kuhn, P., Krueger, C.G., Johnson, W.D., Raskin, I., 2014. Development and phytochemical characterization of high polyphenol red lettuce with anti-diabetic properties 9, 91571. [24] Zahidul, I. M.D., Tanvir, M.D.H., Foysal, H., Mir S.A., Mohammad, A.M., 2016., In-vitro antioxidant and antimicrobial activity of Bougainvillea glabra flower, Research Journal of Medicinal Plant 10(3), 228-236, [25] Ghogar, A., Jiraungkoorskul, k., Jiraungkoorskul, W., 2016. Paper flower, Bougainvillea spectabilis: update properties of traditional medicinal plant. Journal of Natural Remedy. 16, 82-87. [26] Yadav, J.P., Arya, V., Yadav, S., Panghal, M., Kumar, S. D., 2009. Cassia occidentalis: A review on its ethnobotany, phytochemical and pharmacological process. Fitoterapia. 8(1), 223–30. [27] Jawla, S., Kumar, Y., Khan, M.S.Y., 2013. Isolation of antidiabetic principle of Bougainvillea spectabilis Willd(Nyctaginaceae) stem bark. Trop. J. Pharm. Res. 2(1), 759-761 [28] Omar, M., Noman, R. A., Mothana, A. J., Al-Rehaily, A. S., Al qahtani, F. A., Nasr, J. M., Khaled, M. F., Alajmi, M., Al-Said S., 2019. Phytochemical analysis and anti-diabetic, anti-inflammatory and antioxidant activities of Loranthus acaciae Zucc. Grown in Saudi Arabia. Saudi Pharm J. 27(5), 724–730 [29] Dhankhar, S., Sharma, M., Ruhil, S., Balhara, M., Kumar, M., Chhilla, A.K., 2002. Evaluation of antimicrobial and antioxidant activities of Bougainvillea spectabilis. Int J Pharm Pharm Sci, 5, 178–82. [30] Jeong, G.S., Lee, D.S., Song, M.Y., Park, B.H., Kang, D.G., Lee, H.S., Kwon, K.B., Kim, Y.C., 2011. Butein from Rhus verniciflua protects pancreatic beta cells against cytokine-induced toxicity mediated by inhibition of nitric oxide formation. Biol. Pharm. Bull. 34, 97–102. [31] Meng, B., Li, J., Cao, H.., 2013 Antioxidant and antiinflammatory activities of curcumin on diabetes mellitus and its complications. Curr. Pharm. Des. 19, 2101–2113. [32] Girones-Vilaplana, A., Mena, P., Moreno, D.A., Garcia-Viguera, C., 2014. Evaluation of sensorial, phytochemical and biological properties of new isotonic beverages enriched with lemon and berries during shelf life. J. Sci. Food Agric. 94, 1090–1100. [33] Kumar, G.S.P., kasireddy, G.R., Kumar, C.D.A.S., Bhavani, E., 2017. Antidiabetic properties of the methanol leaf extract of Bougainvillea Glabra on alloxan induced diabetic rats; jounal of dental and medical sciences 16(11), 8-13.



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