Isolation, evaluation and characterization of isolated compounds from aqueous extract of Cestrum nocturnum

Chandra Kishore Tyagi, Deenanath Jhade, Sunil Kumar Shah



The aim of present study was to isolate, characeterise and evaluation anticoagulant properties of the isolated compound from aqueous extract of Cestrum nocturnum using aPTT-Activated Partial Thromboplastin Time, PT- Prothrombin Time & TT-Thrombin Time as standard procedures.

From all the result it can be concluded that water extract of Cestrum nocturnumplant was found to be best among all the extract under study and from the isolates of water extract of Cestrum nocturnum plant AE5 was found to be best activity (anti-platelet, Fibrinolytic and anti-coagulant). Further fractionation of water extract generated two isolates AE5a & AE6a and after their pharmacological activity they were found to be potent phytochemical constituents. From the structural elucidation study of AE5a & AE6a were identified as Rutin and quercetin respectively.

From this study it was concluded that the flavoinoidal compound i.e. Rutin and Quercetin were present in the Cestrum nocturnum plant and they both responsible for the anti-platelet, Fibrinolytic and anticoagulamt activity of plant.


Cestrum nocturnum, Thromboplastin, Prothrombin, Rutin, Quercetin.

Full Text:



[1]. Baquar SR. The role of traditional medicine in rural environment. In: Issaq S (ed) Traditional Medicine in Africa. East Africa Educational Publishers Ltd., Nairobi 1995; 141-142.

[2]. Abalson PH. Medicine from plants. Science, 1990; 247: 513-519.

[3]. Lanfranco G. Invited review article on traditional medicine. Electronic Journal of Biotechnology1999; 2: 1-3.

[4]. World Health Organization. General Guidelines for Methodologies on Research and Evaluation of Traditional Medicine. WHO, Geneva, Switzerland 2001.

[5]. Edoega HO, Mbaeble BO and Okwu DE. Phytochemical constituents of some Nigerian medicinal plants, African Journal of Biotechnology. 2005; 4:685-688.

[6]. Rahman KH and Billington D. Dietery supplementation with aged garlic extract inhibits ADP-induced platelet aggregation in humans. Human nutrition and metabolism research communication. 2000;130: 2662-2665.

[7]. Dutta-Roy AK, Crosbie L and Gordon MJ. Effect of tomato extract on human platelet aggregation in vitro, Platelet. 2001;12: 218-227.

[8]. Hutchings A. Zulu medicinal plants: an inventory, 1sted, PMB University of Natal Press, 1992.

[9]. Ichinose, A. Multiple members of the plasminogen-apolipoprotein (a) gene family associated with thrombosis. Biochemistry, 1996; 31, 3113–3118.

[10]. Sofoware A. Medicinal plants and traditional medicine. Spectrum books Ltd. Ibadhan, Nigeria: 1993;289.

[11]. Amrani S. Harnafi H. Gadi D. Mekhfi H. Legssyer A. Aziz M. Martin-Nizard F. Bosca L. Vasorelaxant and anti-platelet aggregation effects of aqueous Ocimum basilicum extracts. Journal of Ethnopharmacology 2009;125(1): 157-162.

[12]. Gadi D and Bnouham M. Parsley extracts inhibits in vivo and ex vivo platelet aggregation and prolongs bleeding time in rats, Journal of ethnopharmacology. 2009;125:170-174.

[13]. Lau AJ, Toh DF, Chua TK, Pang YK, Woo SO and Koh HL. Antiplatelet and antiocoagulant effects of Panaxnoto ginseng. . Journal of Pharmacology. 2009;125: 380-38.

[14]. Goldhaber SZ and Morrison RM. Pulmonary embolism and deep vein thrombosis. Circulation. 2002: 106: 1436–1438.

[15]. Van Wyk B-E, Van Oudtshoom and B, Gerike N. Medicinal plants of South Africa, Briza, Pretoria. 1997; 8(12) 14-20.

[16]. Cooper EL. Drug discovery, CAM and natural products. eCAM 2001;1: 215-217.

[17]. Lindequist U, Niedermeyer T, Jülich WD. The pharmacological potential of mushrooms. eCAM, 2005; 2: 285-299.

[18]. Kokate CK. A text book of Practical pharmacognosy 5thEdition, Vallabh Prakashan New Delhi: 2005;107-111.

[19]. Rashid HU, Rahman MA, Sultana R, Bin Emran T, Islam MS, Chakma JS, Hasan CM. Effects of organic extracts of six Bangladeshi plants on in vitro thrombolysis and cytotoxicity. BMC Complement Altern Med. 2013; 13, 25.

[20]. Mao W, Li H, Li Y, Zhang H, Qi X, Sun H, Chen Y, Guo S. Chemical
characteristic and anticoagulant activity of the sulfated polysaccharide
isolated from Monostroma latissimum (Chlorophyta). Int J BiolMacromol, 2009;44:70–74.

[21]. Sahane RS, Wankhade P. A, Shrungarpure MA. Pharmacological and phytochemical investigation of Cestrum nocturnum leaf extract for antihyperglycemic and antihyperlipidemic activity. International Journal of Research in Pharmacology & Pharmacotherapeutics, 2014;4, 255-268.


  • There are currently no refbacks.

Copyright (c) 2016 Chandra Kishore Tyagi

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

                AR Journals

Street 1st, Gaytri Vihar, Pinto Park, Gwalior, M.P. India

     (Design) 2009-2021


Follow @arjournals on Twitter