HPLC-UV profile of Genista ulicina Spach. (Fabaceae) extracts and in vitro antioxidant activity

Kaoutar Chebbah, Eric Marchioni, Djamel Sarri, Ahmed Menad, Ratiba Mekkiou, Ramdane Seghiri, Ouahiba Boumaza, Samir Benayache, Fadila Benayache

DOI: http://dx.doi.org/10.5138/09750185.1863

Abstract


To perform a qualitative and quantitative analysis of the phenolic and flavonoid contents and evaluate the antioxidant activity of ethyl acetate (EtOAc) and n-butanol (n-BuOH) extracts of the aerial parts of Genista ulicina Spach. from Algeria. The qualitative analysis of plant extracts was carried out by RP-HPLC using UV detector, whereas the quantification of total phenolic and flavonoid contents was completed according to the Folin-Ciocalteu procedure and aluminium chloride colorimetric method respectively. To evaluate the extract's antioxidant activity, Two in vitro antioxidant tests were employed: DPPH and β-carotene bleaching assay. The HPLC/DAD chromatogram showed several peaks indicating the presence of phenolic acids, flavonoids and isoflavonoids in both extracts. The total phenolic content (TPC) ranged from 62.56 and 50.45 mgGAE/g extract, while the total flavonoids content varied between 53.1 and 48.4 mgQE/g extract for EtOAC and n-BuOH respectively. EtOAc extract showed a maximum inhibition value (78.15%) at 150µg/mL using DPPH test and highest antioxidative power (82.42%) using β-carotene bleaching assay comparing with standards. The HPLC-UV analysis showed the richeness of both extracts in phenolic and flavonoid contents. The EtOAc extract exhibited good antioxidant activities comparing to the n-BuOH extract. Thus Genista ulicina could be indicated as a plant of phytopharmaceutical importance.


Keywords


HPLC-DAD; Phenolic compounds; Antioxidant activity; Genista ulicina; Fabaceae.

Full Text:

PDF

References


[1]. Gurib-Fakim A. Medicinal plants: Traditions of yesterday and drugs of tomorrow. Molecular Aspects of Medicine. 2006 ; 27: 1 - 93.

[2]. Yingbin Shen, Hui Zhang, Liling Cheng, Li Wang, Haifeng Qian, Xiguang Qi. In vitro and in vivo antioxidant activity of polyphenols extracted from black highland barley. Food Chemistry. 2016; 194: 1003-1012.

[3]. González-Burgos E, Carretero ME, Gómez-Serranillos MP. Sideritis spp.: Uses, chemical composition and pharmacological activities -A review. Journal of Ethnopharmacology. 2011; 135 (2): 209-225

[4]. Uddin Ghias RA, Siddiqui BS, Khan H, Uzair Ali Shah S, BenHadda T, Mabkhot YN, Farooq U, Khan A. Antinociceptive and anti-inflammatory activities of flavonoids isolated from Pistacia integerrima galls. Complementary Therapies in Medicine. 2016; 25: 132-138.

[5]. Sofidiya MO, Agunbiade FO, Koorbanally NA, Sowemimo A, Soesan D, Familusi T. Antiulcer activity of the ethanolic extract and ethyl acetate fraction of the leaves of Markhamia tomentosa in rats. Journal of Ethnopharmacology. 2014; 157:1-6.

[6]. Nagulsamy P, Ponnusamy R, Thangaraj P. Evaluation of antioxidant, anti-inflammatory and antiulcer properties of Vaccinium leschenaultii Wight: A therapeutic supplement. Journal of Food and Drug Analysis. 2015; 23(3): 376-386.

[7]. Heilmann J, Calis I, Kirmizibekmez H, Schuhly W, Harput S, Sticher O. Radical scavenger activity of phenylethanoid glycosides in FMLP stimulated human polymorphonuclear leukocytes: Structure activity relationships. Planta Med 2000; 66(7): 746-748.

[8]. Calucci L, Pinzono C, Zandomeneghi M, Capocchi A. Effects of gamma-irradiation on the free radical and antioxidant contents in nine aromatic herbs and spices. Journal of Agricultural and Food Chemistry 2003; 51: 927-934.

[9]. Baali N, Belloum Z, Ameddah S, Menad A, Benayache F, Benayache S. Flavonoids screening and hypoglycemic effect of Genista quadriflora Munby in alloxan-induced diabetic rats. Der Pharmacia Lettre 2014 ; 6 (4):194-200.

[10]. Meriane D, Genta-Jouve G, Kaabeche M, Michel S, Boutefnouchet S. Rapid Identification of Antioxidant Compounds of Genista saharae Coss. & Dur. by Combination of DPPH Scavenging Assay and HPTLC-MS. Molecules 2014, 19, 4369-4379;

[11]. Borrelli F, Izzo AA. The Plant Kingdom as a Source of Anti-ulcer Remedies. Phytother. Res. 2000 ; 14 : 581–591.

[12]. Choi EY, Bae SH, Ha MH, Choe SH, Hyeon JY, Choi JI, Choi IS, Kim SJ. Genistein suppresses Prevotella intermedia lipopolysaccharide-induced inflammatory response in macrophages and attenuates alveolar bone loss in ligature-induced periodontitis. Archives of Oral Biology 2016; 62: 70-79

[13]. Rigano D, Russo A, Formisano C, Cardile V, Senatore F. Antiproliferative and cytotoxic effects on malignant melanoma cells of essential oils from the aerial parts of Genista sessilifolia and G. tinctoria. Nat Prod Commun. 2010 5(7):1127-32.

[14]. Boukaabache R, Boumaza O, Mekkiou R, Seghiri R, Benayache S, Benayache F. Preliminary Phytochemical Analysis and Chemical Constituents from Genista aspalathoides Lamk. ssp. erinaceoides (Lois.) M. (Fabaceae). Research journal of pharmaceutical, biological and chemical sciences 2015; 6(2): 551-554.

[15]. Chebbah K, Marchioni E, Menad A, Mekkiou R, Sarri D, Ameddah S, Boumaza Ouahiba,Seghiri Ramdane, Benayache Samir,Benayache Fadila. Preliminary phytochemical screening, analysis of phenolic compounds and antioxidant activity of Genista cephalantha Spach. (Fabaceae). International Journal of phytomedicine 2014; 6(3): 360-368.

[16]. Boukaabache R, Boubekri N, Boumaza O, Mekkiou R, Seghiri R, Sarri D, Zama D, Benayache F, Benayache S. Phytochemical study of ethyl acetate extract and antioxidant activity of Genista quadriflora Munby (Fabaceae). Der Pharmacia Lettre. 2013 ; 5 (6) : 56-59.

[17]. Kerkatou M, Menad A, Sarri D, León F, Brouard I, Bouldjedj R, Chalard P, Ameddah S, Benayache S, Benayache F. Secondary metabolites from Genista aspalathoides Lamk ssp. aspalathoides M. Der Pharmacia Lettre. 2013; 5 (5): 285-289.

[18]. Mekkiou R, Seghiri R, Boumaza O, Sarri D, Chebbah K, Benayache S, Benayache F. Secondary metabolites from Genista ferox. Chem Nat Comp 2012; 48 (4): 710-711.

[19]. Boumaza O, Mekkiou R, Seghiri R, Sarri D, Benayache S, Garcia VP, Benayache S, Benayache F. Secondary metabolites from chloroform extract of Genista tricuspidata. Chem Nat Comp 2011; 47 (2): 277-278.

[20]. Quezel P, Santa S. Nouvelle flore de l’Algérie et des régions désertiques méridionales. Editions du C.N.R.S, Paris. 1962. Tome I. p. 470-475.

[21]. Singleton VL, Orthofer R, Lamuela-raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol 1999; 299: 152-178.

[22]. Quettier DC, Gressier B, Vasseur J, Dine T, Brunet C, Luyckx MC, Cayin JC, Bailleul F, Trotin F. Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. J. Ethnopharmacol 2000; 72: 35-42.

[23]. Magalhães LM, Santos M, Segundo MA, Reis S, Lima JLFC. Automatic method for determination of total antioxidant capacity using 2;2-diphenyl-1- picrylhyrazyl assay. Anal. Chim. Acta. 2006; 558: 310-318.

[24]. Miraliakbari H, Shahidi F. Antioxidant activity of minor components of tree nut oils. Food Chem. 2008; 111 (2): 421-7.

[25]. Luczkiewecz M, Glod D, Baczek T, Bucinski A. LC-DAD-UV and LC-MS for the analysis of isoflavones and flavones from in vitro and in vivo biomass of Genista tintoria L. Chromatographia. 2004; 60: 179-185.

[26]. Mabry TJ, Markham KR, Thomas MB. The systematic identification of flavonoids. Ed. New York, Springer-Verlag, 1970, 354p.

[27]. Al-Mamary M, Al-Meeri A, Al-Habori M. Antioxidant activities and total phenolics of different types of honey. Nutrition Res, 2002; 22:1041-1047.


Refbacks

  • There are currently no refbacks.
';



Copyright (c) 2016 Kaoutar Chebbah, Eric Marchioni, Djamel Sarri, Ahmed Menad, Ratiba Mekkiou, Ramdane Seghiri, Ouahiba Boumaza, Samir Benayache, Fadila Benayache

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

                AR Journals

Street 1st, Gaytri Vihar, Pinto Park, Gwalior, M.P. India

              Copyright@arjournals.org (Design) 2009-2021

 

Follow @arjournals on Twitter