Possibilities of developing novel potent antitumor agents from the leaves of Cryptomaria japonica

Laxmi Rani Basu, Amiyangshu De, Pradipta Sarkar, Prithviraj Karak, Sujata G Dastidar

DOI: http://dx.doi.org/10.5138/09750185.1860

Abstract


During past few decades cancer has remained as the largest cause of mortality worldwide and number of patients suffering from cancer has been increasing at a fast rate. Hence medical research during the last few decades has been concentrating on identification and characterization of new synthetic pharmacological compounds to overcome this enormous problem. Leaf extracts of coniferous plant Cryptomeria japonica being known for their strong antibacterial and antifungal functions were selected to determine their antitumor/anticancer potentialities.

Methanolic extract of leaves were tested to determine its antitumor action in standard murine model of Ehrlich Ascites Carcinoma (EAC). Graded doses of the extract were given intraperitoneally to batches of mice, who received EAC challenge after 3hr. Treatment with same amounts of extract was continued for 9 consecutive days. Protective capacity of the leaf extract was evaluated in animals.

Statistically significant protection was observed  with respect to different parameters including  tumor volume , tumor cell count , viable tumor cell count, non- viable tumor cell count , mean survival time and increase in life span. Simultaneously hematological parameters were restored in treated mice vis-à-vis untreated control animals. Furthermore, the extract revealed distinct cytotoxic property, which may be the relevant reason of its anticancer/antitumor function.

This study shows efficacy of methanolic extract of leaves of Cryptomeria japonica as a probable antitumor/anticancer agent. Phytochemical analysis of the extract showed presence of flavonoids, which are known to possess significant anticancer activity. Thus there is a definite possibility of developing novel anticancer drugs from such plant products

Keywords


Cancer, Anticancer agent, Antitumor agent, Plant extract, Cryptomaria japonica

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References


[1]. De Martel C, Ferlay J, Franceschi S, et al. Global burden of cancers attributable to infections in 2008: a review and synthetic analysis. Lancet Oncol. 2012; 13 : 607.

[2]. WHO Media Center, Cancer, Fact sheet No. 297 February 2015.

[3]. Xia M , Wang D, Wang M, Tashiro S, Onodera S, Minami M. Dracorhodin perchlorate induces apoptosis via activation of caspases and generation of reactive oxygen species. J Pharmacol Sci. 2004; 95 : 273.

[4]. Shah U, Shah R, Acharya S, Acharya N. Novel anticancer agents from plant sources. Chinese J Natural med. 2013; 11:16.

[5]. Hartwell JL. Plants used against cancer, Lawrence, Massachusetts, USA , 1982 709.

[6]. Graham JG, Quinn ML, Fabricant DS, Farnsworth NR. Plants used against cancer - an extension of the work of Jonathan Hartwell. J Ethnopharmacol. 2000; 73 : 347.

[7]. Kuttan G , Kumar KB, Guruvayoorappan C, Kuttan R. Antitumor, anti-invasion, and antimetastatic effects of curcumin. Adv Exp Med Biol. 2007; 595:173.

[8]. Hatcher H, Planalp R, Cho J, Torti FM, Torti SV. Curcumin: from ancient medicine to current clinical trials. Cell Mol Life Sci. 2008; 65:1631.

[9]. Cragg GM, Newman DJ. Plants as a source of anti-cancer agents. J Ethnopharmacol. 2005; 100 : 72.

[10]. Hsu YL, Chen CY, Lin IP, Tsai EM, Kuo PL, Hou MF. 4-Shogaol, an active constituent of dietary ginger, inhibits metastasis of MDA-MB-231 human breast adenocarcinoma cells by decreasing the repression of NF-κB/Snail on RKIP. J Agric Food Chem. 2012; 60:852.

[11]. Kim JB , Koo HN, Joeng HJ, Lyu YS, Park SG, Won JH, Kim YK, Hong SH, Kim HM. Induction of apoptosis by Korean medicine Gagam-whanglyun-haedoktang through activation of caspase-3 in human leukemia cell line, HL-60 cells. J Pharmacol Sci. 2005; 97:138.

[12]. Búfalo MC, Candeias JMG, Sforcin JM. In vitro Cytotoxic Effect of Brazilian Green Propolis on Human Laryngeal Epidermoid Carcinoma (HEp-2) Cells. Evid Based Complement Alternat Med. 2009; 6 : 483.

[13]. Chen Y, Yang SZ, Zhao MS, Ni BY, Liu L,Chen XY. Demographic genetic structure of Cryptomeria japonica var. sinensis in Tianmushan Nature Reserve, China. J Integr Plant Biol. 2008; 50:1171.

[14]. Cheng SS, Lin HY, Chang ST. Chemical composition and antifungal activity of essential oils from different tissues of Japanese Cedar (Cryptomeria japonica). J Agric Food Chem, 2005; 53:614.

[15]. Lee JH, Lee BK, Kim JH, Lee SH, Hong SK. Comparison of chemical compositions and antimicrobial activities of essential oils from three conifer trees Pinus densiflora, Cryptomaria japonica and Chamaecyperus obtuse. J Micro. Biotech. 2009; 19:391.

[16]. Wang SY, Lai C, Chu, FH, Lin, CT, Shen SY, Chang ST. Essential oil from the leaves of Cryptomeria japonica acts as a silverfish (Lepisma saccharina) repellent and insecticide. J Wood Sci. 2006; 52:522.

[17]. Takei M, Umeyama A, Arihara S. Epicubenol and Ferruginol induce DC from human monocytes and differentiate IL-10-producing regulatory T cells in vitro. Biochem Biophys Res Commun. 2005; 337: 730

[18]. Ghosh MN. Fundamentals of experimental pharmacology. 2nd ed. Calcutta, India: Scientific Book Agency; 1984. p. 192.

[19]. Dagli MLZ, Guerra JL, Saldiva PHN. An experimental study on the lymphatic dissemination of the solid Ehrlich tumor in mice,Braz. j vet res anim sci. 1992; 29:97

[20]. Wintrobe MM, Lee GR, Boggs DR, Bithel TC, Athens JW, Foerester J. Clinical Pharmacology. 5th ed. Philadelphia, PA, USA: Les and Febiger; 1961.p.326.

[21]. Blood FR , D'Amour FE. The Manual for Laboratory Work in Mammalian Physiology. 3rd ed. Chicago, Illinois, USA: The University of Chicago Press; 1965.p.4.

[22]. Boyse E, Old E, Chouroubnkov I. Eisen M, editor. Methods in Medical Research. Ist vol. Chicago, Illinois, USA : Year Book Medical Publishers; 1964.p.39.

[23]. Fecchio D, Sirois P, Russo M, Jancar S. Studies on inflammatory response induced by Ehrlich tumor in mice peritoneal cavity. Inflammation, 1990; 14 : 125.

[24]. Shimizu M, Azuma C, Taniguchi T, Murayama T. Expression of cytosolic phospholipase A2 in murine C12 cells, a variant of L929 cells, induces arachidonic acid release in response to phorbol myristate acetate and Ca2+ ionophores, but not to tumor necrosis factor ɑ. J Pharmacol Sci. 2004; 96:324.

[25]. Segura JA, Barbero LG, Marquez J. Ehrlich ascites tumour unbalances splenic cell population and reduces responsiveness of T cells to Staphylococcus aureus enterotoxin B stimulation. Immunology Lett. 2000; 74:111.

[26]. Krzyzanski W, Ruix JJP. Lifespan based indirect response models. J Pharmacokinet Pharmacodyn. 2012; 39:109.

[27]. Miller CB, Jones RJ, Piantadosi S, Abeloff MD, Spivak JL. Decreased erythropoietin response in patients with the anemia of cancer. N Engl J Med. 1990; 322:1689.

[28]. Hida T, Kozaki K, Muramatsu H, Masuda A, Shimizu S, Mitsudomi T, Sugiura T, Ogawa M, Takahashi T. Cyclooxygenase-2 inhibitor induces apoptosis and enhances cytotoxicity of various anticancer agents in non-small cell lung cancer cell lines. Clin Cancer Res, 2000; 6: 2006.

[29]. Kandaswami C, Lee LT, Lee PP, Hwang JJ, Ke FC, Huang YT, Lee MT. The antitumor activities of flavonoids. In Vivo. 2006; 19: 895.


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