In-vitro scavenging activity and acute toxicity study of methanol leaves extract and fractions of Lophira lanceolata Tiegh. Ex Keay (Ochnaceae) in rats.

Jean-Baptiste N. Oussou, Isaac J. Asiedu-Gyekye, Adou F. Yapo, Benoit Banga N'guessan, Patrick Amoateng, Leandre K Kouakou, Isaac K Asante, Etienne E Ehile

DOI: http://dx.doi.org/10.5138/09750185.1855

Abstract


The present study aims at comparing in-vitro scavenging activities of methanol extract and fractions of Lophira lanceolata leaves, and also to study the oral acute toxicity of the ethyl acetate fraction.

Petroleum ether, dichloromethane, ethyl acetate, butanol and water fractions were prepared from the methanol extract of the plant. The scavenging activity of DPPH (2, 2-Diphenyl-1-picrylhydrazyl), the total phenolic (TPC) and total flavonoid (TFC) contents of the extract and fractions were determined by spectrophotometrical methods using gallic acid (GA) and quercetin (Qu) as reference antioxidant. The fifty percent inhibitory concentration (IC50), fifty percent effective concentration (EC50) and the antiradical power (ARP) were determined for all extract and fractions. An acute toxicity study using a single oral dose of 5000 mg/kg of the ethyl acetate fraction of the plant was conducted in female Albino rats following the OECD (420) Guidelines. Blood samples were collected for hematological and biochemical analysis. Histopathological examinations of the heart, kidney and liver were performed.

The results showed that the ethyl acetate fraction of L. lanceolata had the highest free radical scavenging activity of DPPH (IC50=1,43; EC50= 0.07 and ARP = 14,28)  and also contain the highest amount of total phenols (14,4±0,02 mg of GA equivalent/g of plant fraction) and total flavonoids (93,3±0,04 mg of Qu equivalent/g of plant fraction) as compared to other fractions and ascorbic acid (IC50=5,82; EC50= 3.44; ARP=3,44). The hematological parameters and the lipid profile didn’t show any major change compared to the control group. However, a significant increase of aspartate aminotransferase (AST, p<0.001) and alanine aminotransferase (ALT, p<0.001) showed that the ethyl acetate fraction of the methanol extract of L. lanceolata leaves might not totally be safe for consumption, in the conditions of our experiment.


Keywords


antioxidant activity; acute toxicity; hematology; biochemistry; histopathology; Lophira lanceolata.

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References


[1]. White L, Abernethy K. Guide de la végétation de la Réserve de la Lopé, Libreville: ECOFAC Gabon 1996; 224 p.

[2]. Adjanohoun ES, Ake AL. Contribution au recensement des plantes médicinales de Côte d’Ivoire (Tome 1), Centre National de Floristique, Abidjan, Côte d’Ivoire 1979; 356 p.

[3]. Arbonier M. Arbres, arbustes et lianes des zones sèches d’Afrique de l’ouest. CIRAD, MNHN, UICN 2000; 425‐427.

[4]. Adjanohoun EJ, Aké assi L, Floret JJ, Ginko S, Koumare M, Ahyi AMR, Raynal J. Médecine traditionnelle et pharmacopée : contribution aux études ethnobotaniques et floristiques au Mali, Paris, ACCT 1981; 291 p.

[5]. Ghogomu RT., Sondengam BL., Martin MT, Bodo B. Lophirones D and E: two new cleaved biflavonoids from Lophira lanceolata. J Nat Pod 1989; 52: 284.

[6]. Kouakou K L, Bléyéré NM., Oussou NJ-B, Konan BA, Amonkan KA, Abo KJ-C, Yapo AP, Ehilé EE. Effects of leaf decoction from Lophira lanceolata Tiegh. Ex Keay (Ochnaceae) on arterial blood pressure and electrocardiogram in anesthetized rabbits. Pharma innovation J 2013; 2 (9) 66-73.

[7]. Igboeli N, Onyeto CA, Okorie AN, Mbaoji FN, Nwabunike IA, Alagboso DI. Antidiarrheal activity of methanol leaf extract of Lophira Lanceolata Tiegh (Ochnaeceae), Merit Res. J. Environ. Sci. Toxicol 2015; 3(4): 059-064.

[8]. Onyeto CA, Akah PA, Nworu CS, Okoye TC, Okorie NA, Mbaoji FN, Nwabunike IK, Okumah N, Okpara O. Antiplasmodial and antioxidant activities and methanol extract of the fresh Lophira lanceolata (Ochnaceae). Afri J Biotechnol 2014; 13 (16): 1731-1738.

[9]. Oussou NJ-B, Eric Boakye-G, Kouakou KL, N’guessan BB, Amoateng P, Yapo AF, Asiedu-Gyekye IJ, Ehilé EE. Comparative effect of different extracts and fractions from lophira lanceolata tiegh. Ex keay (ochnaceae) leaves on arterial blood pressure and heart rate in anaesthetized cats, WJPPS 2015; 4(11): 55-68.

[10]. Audu SA, Mohammed I, Kaita HA. Phytochemical screening of the leaves of Lophira lanceolata (Ochnaceae). Life Sci J 2007; (4):75-79.

[11]. Audu SA, Abdulraheem OR, Sule IM, Ilyas M, Haruna AK, Sikira AS. Acute toxicity studies of Lophira lanceolata leaf extract. RJPBCS 2011; 2(1): 629-636.

[12]. WHO. Promotion du rôle de la médecine traditionnelle dans le système de santé.

Stratégie de la région africaine 2001; pp. 17-19.

[13]. Martinez-Valverde I, Periago MJ, Ros G. Significado nutricional de los componentes fenolicos de la dieta. Arch Latinoam Nutr 2000; 50: 5-18.

[14]. de Groot H, Rauen U. Tissue injury by reactive oxygen species and the protective effects of flavonoids. Fundam Clin Pharmacol 1998; 12:249–55.

[15]. Branien AL. Toxicology and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene. J Am Oil Chem Soc 1975; 52(2):59-63.

[16]. Zirihi GN, Kra AKM, Bahi C, Guédé-Guina F. Plantes médicinales immunostimulantes: critères de sélection, techniques rapides d’extraction des principes actifs et méthodes d’évaluation de l’activité immunogène. Rev Pharm Méd Trad Afr 2003; 17: 131-8.

[17]. Brand-Williams W, Cuvelier ME, Berset C. Use of free radical method to evaluate antioxidant activity. Lebensmittel-Wissenschaft und-Technol 1995; 28:25–30.

[18]. Bursal E, Gulcin I. Polyphenol contents and in vitro antioxidant activities of lyophilized aqueous extract of kiwifruit (Actinidia deliciosa). Food Res Int 2011; 44:1482–1489.

[19]. Kroyer GT. Red clover extract as antioxidant active and functional food ingredient. Innovat Food Sci Emerg Tech 2004; 5: 101-105.

[20]. Prakash D, Upadhyay G, Gupta C, Pushpangadan P, Singh KK. Antioxidant and free radical scavenging activities of some promising wild edible fruits. Int Food Res J 2012; 19 (3): 1109-1116.

[21]. Harborne JB. Phytochemical methods Second edition, By Chapman and Hall, London and New York 1984; 37(3):19-25.

[22]. Bekro Y, Bekro J, Boua BB, Tra-Bi F, and Ehilé EE. Etude ethnobotanique et screening phytochimique de Caesalpinia benthamiana (Baill.) Herend et Zarrucchi (Caesalpiniaceae). Sci & Nat 2007; 2(4): 217‐225.

[23]. Park YK, Koo MH, Ikegaki M, Contado JL. Comparison of the flavonoid aglycone contents of Apismelli ferapropolis from various regions of Brazil. Arq Biol Tecnol 1997; 40, 97-106.

[24]. OECD. Organization for Economic Cooperation and Development Guidelines, for Testing of Chemicals: Acute Oral Toxicity-Fixed Dose Procedure, 2001; 420 P.

[25]. Sharififar F, Dehghn-Nudeh G, Mirtajaldini M. Major flavonoids with antioxidant activity from Teucrium polium L. Food Chem 2009; 112:885–888.

[26]. Khan RA, Khan MR, Sahreen S. Assessment of flavonoids contents and in vitro antioxidant activity of Launaea procumbens. Chem Central J 2012; 6:43.

[27]. Cao G, Sofic E, Prior RL. Antioxidant and pro-oxidant behavior of flavonoids: Structure activity relationships. Free Rad Biol Med 2009; 22:749–760.

[28]. Matejić JS, Džamić AM, Mihajilov-Krstev TM, Ranđelović VN, Krivošej ZĐ, Marin PD. Total phenolic and flavonoid content, antioxidant and antimicrobial activity of extracts from Tordylium maximum, J App Pharm Sci 2013; 3 (1): 055-059.

[29]. Yingming P, Ping L, Hengshan W, Min L. Antioxidant activities of several chinese medicinal herbs. Food Chem 2004; 88: 347-350.

[30]. Louli V, Ragoussis N, Magoulas K. Recovery of phenolic antioxidants from wine industry by products. Bioresour Technol 2004; 92, 201–208.

[31]. Ranelletti FO, Maggiano N, Serra FG. Quercetin inhibits p21-ras expression in human colon cancer cell lines and in primary colorectal tumors. Inter J Cancer 1999; 85: 438-445.

[32]. Langley-Evans C. Antioxidant potential of black and green tea determined using the ferric reducing power (FRAP) assay. Intl J Food Sci Nut 2000; 51:181–188.

[33]. Alasalvar C, Karamac M, Amarowicz R, Shahidi F. Antioxidant and antiradical activities in extracts of Hazelnut kernel (Corylusavellana L.) and hazelnut green leafy cover. J Agric Food Chem 2009; 54:4826-4832.

[34]. Oyedemi SO, Bradley G, Afolayan AJ. Toxicological effects of the aqueous extract of Strychnos henningsii Gilg in Wistar rats. J Nat Pharm 2010; 1(1): 33-39.

[35]. Stephen HR, Reich PR. Hematology: Pathologic basis for clinical practice, Third edition Little Brown, 2000; 460 p.

[36]. Ghasi S, Egwuibe C, Achukwu PU, Onyeanusi JC. Assessment of the medical benefit in the folkloric use of Bryophyllum Pinnatum leaf among the Igbos of Nigeria for the treatment of hypertension. Afr J Pharm Pharmacol 2011; 5(1): 83-92.

[37]. Hilaly E, Israeli ZH, Lyoussi B. Acute and chronic toxicological studies of Ajuva iva in experimental animals. J Ethnopharmacol 2004; 91(1): 43-50.

[38]. Lameire N, Biesen VW, Vanholder R. Acute renal failure. The Lancet 2005; 365 (9457): 417-430.

[39]. Sellers RS, Morton D, Michael B. Society of Toxicology Pathology position paper: organ weight recommendations for Toxicology studies. Toxicol Pathol 2007; 35(5): 751-755.


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