Effects of Lemon balm essential oil application on atopic dermatitis-like immune alterations in mice

Jian-Rong Zhou, Eri Fujiwara, Yuuki Nakamura, Mihoko N. Nakasima, Kazumi Yokomizo


Lemon balm oil has been used as anti-inflammation agent, but there has been no scientific study to date on the possible effects of Lemon balm oil on alleviating atopic dermatitis. BALB/c mice were sensitized with 2, 4-dinitrochlorobenzene for atopic dermatitis induction. Thereafter, Lemon balm oil was applied on the dorsal skin for 4 weeks. Scratching behavior was measured. Blood and spleen were collected for measuring IgE and cytokine, respectively. Serum IgE levels were significantly lowered in 3% Lemon balm oil application group after 3-week application. The Lemon balm oil application showed a significantly lower spleenocyte interleukin-10 level and an increasing tendency in Interferon- level compared with that of saline application. Scratching frequency of the Lemon balm oil application group had a lower tendency than the control groups. This study may demonstrate Lemon balm oil’s immunoregulatory potential for alleviating atopic dermatitis through influencing of Th2 / Th1 cell activation.


atopic dermatitis, cytokine, Lemon balm oil, IgE, mice

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Matsumoto M, Ra C, Kawamoto K, Sato H, Itakura A, Sawada J, Ushio H, Suto H, Mitsuishi K, Hikasa Y, Matsuda H. IgE hyperproduction through enhanced tyrosine phosphorylation of Janus kinase 3 in NC/Nga mice, a model for human atopic dermatitis. J Immunol. 1999;162:1056-1063.

Takakura M, Takeshita F, Aihara M, Xin KQ, Ichino M, Okuda K, Ikezawa Z. Hyperproduction of IFN-γ by CpG oligodeoxynucleotide-induce exacerbation of atopic dermatitis-like skin lesion in some NC/Nga mice. J Invest Dermatol. 2005;125:1156- 1162.

Del Rosso J, Friedlander SF. Corticosteroids: options in the era of steroid-sparing therapy. J Am Acad Dermatol. 2005, 53 (Suppl 1):50-58.

Norris DA. Mechanisms of action of topical therapies and the rationale for combination therapy. J Am Acad Dermatol. 2005, 53 (Suppl 1):17-25.

Buckle J. The role of aromatherapy in nursing care. Nurs Clin North Am. 2001;36:57-72.

Cerrato P. Aromatherapy: Is it for real? Regist Nurse 1998;61:51-52.

Carnat AP, Carnat A, Fraisse D, Lamaison JL. The aromatic and polyphenolic composition of lemon balm (Melissa officinalis L. subsp. officinalis) tea. Pharmaceu Acta. Helvetiae 1998;72:301-305.

Herodez SS, Hadolin M, Skerget M, Knez Z. Solvent extraction study of antioxidants from Balm (Melissa officinalis L.) leaves. Food Chem. 2003;80:275-282.

Salah SM, Jäger. AK Screening of traditionally used Lebanese herbs for neurological activities. J Ethnopharmacol. 2005;97:145-149.

Lawless J. The encyclopedia of essential oils. Dorset Shaftesbury: ElementBooks. 1992.

Kennedy DO, Wake G, Savelev S, Tildesley NT, Perry EK, Wesnes KA, Scholey AB. Modulation of mood and cognitive performance following acute administration of single doses of Melissa officinalis (Lemon balm) with human CNS nicotinic and muscarinic receptor-binding properties. Neuropsychopharmacol. 2003;28:1871-1881.

Müller SF, Klement S. A combination of valerian and lemon balm is effective in the treatment of restlessness and dysomnia in children. Phytomed. 2006;13:383–387.

Simmen U, Kelber O, Okpaneji SN, Jaeggi R, Buetler B, Weiser D. Binding of STW 5 (Iberogast) and its components to intestinal 5-HT, muscarinic M3 and opioid receptors. Phytomed. 2006;13:51-55.

Schemann M, Michel K, Zeller F, Hohenester B, Rühl A. Region-specific effects of STW 5 (Iberogast) and its components in gastric fundus, corpus and antrum. Phytomed. 2006;13:90-99.

Sandraei H, Ghannadi A, Malekshahi K. Relaxant effect of essential oil of Melissa officinalis and citral on rat ileum contrations. Fitoterapia 2003;74:445-452.

Allaverdiyev A, Duran N, Ozguven M, Koltas S. Antiviral activity of the volatile oils of Melissa officinalis L. against Herpes simplex virus type-2. Phytomed. 2004;11:657-661.

Schnitzler P, Schuhmacher A, Astani A, Reichling J. Melissa officinalis oil affects infectivity of enveloped herpes viruses. Phytomed. 2008;15:734-740.

Janina D, Elzbieta A. The effect of the Melissa officinalis extract on immune response in mice. Acta Poloniae pharmaceutica 2003;60:467-470.

Lin JT, Chen YC, Lee YC, Hou CWR, Chen FL, Yang DJ. Antioxidant antiproliferative and cyclooxygenase-2 inhibitory activities of ethanolic extracts from lemon balm (Melissa officinalis L.) leaves. LWT-Food Sci Technol. 2012;49:1-7.

Guginski G, Luiz AP, Silva MD, Massaro M, Martins DF, Chaves J, Mattos RW, Silveira D, Ferreira VM, Calixto JB, Santos AR. Mechanisms involved in the antinociception caused by ethanolic extract obtained from the leaves of Melissa officinalis (lemon balm) in mice. Pharmacol Biochem Behav. 2009;93:10-16.

Duke JA. Handbook of medicinal herbs. BocaRaton FL: CRCPress. 2002.

Ippoushi K, Yamaguchi Y, Itou H, Azuma K, Higashio H. Evaluation of inhibitory effects of vegetables and herbs on hyaluronidase and identification of rosmarinic acid as a hyaluronidase inhibitor in lemon balm (Melissa officinalis L.). Food Sci Technol. Res. 2000;6:74-77.

Kakegawa H, Matsumoto H, Satoh T. Inhibitory effects of hydrangenol derivatives on the activation of hyaluronidase and their an tiallergic activities. Planta Medica. 1988;54:385-389.

He D, Zhou A, Wei W, Nie L, Yao S. A new study of the degradation of hyaluronic acid by hyaluronidase using quartz crystal impedance technique. Talanta. 2001;53:1021-1029.

Lee SH, Baek SJ, Kim HA, Heo Y. 2, 4- initrochlorobenzeneinduced atopic dermatitis like immune alteration in mice. J Toxicol Public Health 2006;22:357-364.

Inagaki N, Nagao M, Igeta K, Kawasaki H, Kim JF, Nagai H. Scratching behavior in various strains of mice. Skin Pharmacol. Appl Skin Physiol. 2001;14:87-96.

Inagaki N, Igeta K, Shiraishi N, Kim JF, Nagao M, Nakamura N, Nagai H. Evaluation and characterization of mouse scratching behavior by a new apparatus, MicroAct Skin Pharmacol Appl Skin Physiol. 2003;6:165-175.

Yamashita S. A skin external preparation containing plant extract and used for treating atopic dermatitis, Jpn Kokai Tokkyo Koho. 1996.

Leighton Harry J, Frangakis Crist J. Methods and compositions for treating inflammation of skin. PCT Int Appl. 2011.

Koblenzer CS. Itching and the atopic skin. J. Allergy Clin Immunol. 1999;104:109-113.

Wahlgren CF. Itch and atopic dermatitis: an overview. J Dermatol. 1999;26:770-779.

Tamura T, Amano T, Ohmori K, Manabe H. The effects of olopatadine hydrochloride on the number of scratching induced by repeated application of oxazolone in mice. Eur J pharmacol. 2005;524:149-154.

Hăncianu M, Aprotosoaie AC, Gille E, Poiată A, Tuchiluş C, Spac A, Stănescu U. Chemical composition and in vitro antimicrobial activity of essential oil of Melissa officinalis L. from Romania. Rev Med Chir Soc Med Nat Iasi. 2008;112:843-847.

Lin CT, Chen CJ, Lin TY, Tung JC, Wang SY. Anti-inflammation activity of fruit essential oil from Cinnamomum insularimontanum Hayata. Bioresour Technol. 2008;99:8783-8787.

Lee J, Jung E, Koh J, Kim YS, Park D. Effect of rosmarinic acid on atopic dermatitis. J Dermatol. 2008;35:768-771.


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