Effect of Phyllanthus polyphyllus extract on DNA binding studies

Swapna G, Bharathi K, Rajkapoor B, KVSRG Prasad

Abstract


Phyllanthus polyphyllus has demonstrated potent in vitro anticancer activity against various tumor cell lines. For a better understanding of the mechanism of action, binding studies of methanolic extract of Phyllanthus polyphyllus (PP) with Calf Thymus (CT) DNA were studied using absorption spectroscopy, viscometry and cyclic voltammetry. PP displayed binding properties to the CT-DNA and was found to interact with CT-DNA through intercalation, as demonstrated by a hypochromic effect and blue-shift in the UV spectra. An increase in the viscosity of CT-DNA was observed. The changes in the current and potential in cyclic voltammetric experiments demonstrate intercalative interaction between the PP and the CT-DNA.


Keywords


Phyllanthus polyphyllus; Absorption Spectroscopy; Viscometry; Cyclic Voltammetry; Calf Thymus DNA; Intercalation.

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References


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