In vitro antioxidant, antibacterial and membrane stabilizing activity of plant extract from Chrysanthemum fontanesii

amel amrani, Boubekri Nassima, Benaissa Ouahiba, Zama Djamila, Benayache fadila, Benayache Samir


Antioxidant activities, antibacterial and membrane stabilizing activity of butanolic extract from leaves of Chrysanthemum fontanesii were investigated. The inhibition of the formation of malondialdehyde (MDA) in vitro and the scavenging of DPPH were assayed .The phenolic content of the extract was determined. The experimental results show that butanolic extract have antioxidant activity in vitro. The extracts showed a high antioxidant effect, especially scavenging of DPPH anions and inhibition of lipid peroxidation. Antioxidant activities were compared to ascorbic acid. The total content of phenolic compounds was 349 µg of gallic acid equivalents/mg extract. The butanolic extract was effective against microorganisms and against heat-as well as hypotonic solution-induced haemolysis of erytrocytes in vitro.


Chrysanthemum fontanesii; Antioxidant activity; Phenolic compounds; antibacterial activity; membrane stabilizing activity.

Full Text:



. Mccord JM. The evolution if free radicals and oxidative stress. Am. J. Med 2000; 108: 652–9.

. Sharififar F., Moshafi M.H., Mansour S.H., Khodashenas M., Khoshnoodi M. In vitro evaluation of antibacterial and antioxidant activities of the essential oil and methanol extract of endemic Zataria multiflora Boiss. Food Control 2007; 18: 800–805.

. Ashok BT, Ali R. The aging paradox: free radical theory of aging. Exp Gerontol 1999; 34:293–303.

. Hagerman AE, Riedl KM, Jones GA, Sovik kn, Ritchard NT, Hartzfeldt PW. High molecular weight plant polyphenolics (tannins) as biological antioxidants. J. Agric. Food Chem 1998; 46: 1887–92.

. Krings U, Berger RG. Antioxidant activity of some roasted foods. Food Chem 2001;72: 223–229.

. Wang H, Dong Gao X, Zhou GC, Cai L., Yao WB. In vitro and in vivo antioxidant activity of aqueous extract from Choerospondias axillaris fruit. Food Chem 2008; 106:888-895.

. Halliwell B. Free radicals, antioxidants, and human disease: curiosity, cause, or consequence? Lancet 1994; 344:721–4.

. Owen RW, Giacosa A, Hull WE, Haubner R, Spiegelhalder B, Bartsch H. The antioxidnt/anticancer potential of phenolic compounds isolated from olive oil. Eur J of Cancer 2000; 36: 1235–47

. Sala A, Recio MD, Giner RM, Manez S, Tournier H, Schinella G, Rios JL. Anti-inflammatory and antioxidant properties of Helichrysum italicum. J. Pharm. Pharmacol 2002; 54: 365–71.

. Mathew S, Abraham TE. In vitro antioxidant activity and scavenging effects of Cinnamomum verum leaf extract assayed by different methodologies. Food and Chemical Toxicology 2006; 44: 198–206

. Chandini KS, Ganesan P, Bhaskar N. In vitro antioxidant activities of three selected brown seaweeds of India. Food Chemistry 2008; 107: 707–713

. Amrani A, Zama D, Boubekri N, Benaissa O, Meraihi Z, Benayache F, Benayache S, Bettuzzi S. The protective effect of Chrysanthemum fantanesii extract, vitamin E and C on sodium valproate-induced embryotoxicity in pregnant mice. Journal of Medicinal Plants Research 2013; 6: 3535-3544.

. Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. In: Packer L, editor. Methods in enzymol: oxidant and antioxidants (part A), 299. San Diego, CA: Academic Pres 1999; 152-78.

. Braca A., DE Tommasi N., Di Bari L., Pizza C., Politi M., Morelli I. Antioxidant principales from Bauhinia terapotensis. J. Nat. Prod 2001; 64:892-895.

. Cao U., Ikeda I. Antioxidant activity and antitumor activity (in vitro) of xyloglucan selinious ester and surfated xyloglucan. Int.J. Biol. Macromol 2009; 45:231-235.

. Chakraborty M., Mitra A. The antioxidant and antimicrobial properties of the methanolic extract from Cocos nucifera mesocarp. Food Chem 2008: 107: 994–99.

. Mayachiew P., Devahastin S. Antimicrobial and antioxidant activities of Indian gooseberry and galangal extracts. LWT 2008; 41: 1153–9.

. Rajaei A., Barzegar M., Mobarez A. M., Sahari M.A, Esfahani Z. H. Antioxidant, anti-microbial and antimutagenicity activities of pistachio (Pistachia vera) green hull extract. Food Chem. Toxicol 2010; 48: 107–112.

. Shinde U.A., Phadke A.S., Nair A.M., Mungantiwar A.A., Dikshit V.J., SARAF V.O. Membrane stabilizing activity–a possible mechanism of action for the antiinflammatory activity of Cedrus deodara wood oil. Fitoterapia 1999; 70: 251-7.

. Rice-Evans C.A., Miller N.J., Bolwell P.G., Bramley P.M., Pridh JB. The relative antioxidant activities of Plant-derived polyphenolics flavonoids. Free Radical Res 1995; 22: 375-383.

. Janero D.R. Malondialdehyde and thiobarbituric acid reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Radical Biol. Med 1990; 9: 515-40.

.Yoshikawa T., Naito Y., Kondo M. Food and diseases. In M. Hiramatsu, T. Yoshikawa, & M. Inoue (Eds.), Free radicals and diseases. New York Plenum press 1997; 11–9.

. Li J., Zhang M., Zheng T. The in vitro antioxidant activity of lotus germ oil from supercritical fluid carbon dioxide extraction. Food Chem 2009; 115: 939–944.

.Sadique J., Al-Rqobah W.A., Bugharlth M.E., El-Gindy A.R. The bioactivity of certain medicinal plants on the stabilization of RBC membrane system. Fitoterapia 1989; LX: 525-32.

.Oyedapo O.O., Akindele V.R., Okunfolami K.O. Effects of the extracts of the Olax Subs corpiodes and Aspila africana on bovine red blood cells. Phytotherapy Res 1997; 11: 305.

. Olugbenga M., Fafunso M.A., Makinde J.M. Membrane stabilizing activity: A possible mechanism of action for the anti- inflammatory property of Gongronema latifolium leaves. Int. J. Biomed.Health Sci 2005; 1: 1-4.

. Kumar PE., Sadique J. The biochemical mode of action of Gynamdropsis gynandra in inflammation. Fitopterapia 1987; L VIII: 379-85.

. Oyedapo O.O., Akinpelu B.A., Orefuwa S.O. Ant-inflamatory effect of Theobroma cacao, root extract. Trop. Med. Plants 2004; 5: 161- [30].

. Arora A., Byrem T M., Nair MG. Strasburg GM. Modulation of liposomal membrane fluidity by flavonoids and isoflavonoids. Arch Biochem Biophys 2000 373: 102–9.

. Saija A., Scalese M., Lanza M., Marzullo D., BOnina F., Castelli F. Flavonoids as antioxidant agents: importance of their interaction with biomembranes. Free Radic Biol Med 1995; 19: 481–6

. Sousa A., Ferreira I.C.F.R., Calhelha R., Andrade P.B., Valent P., Seabra R., Estevinho L., BENTO A., PEREIRA J.A. Phenolics and antimicrobial activity of traditiona stoned table olives ‘‘alcaparra”. Bioorg. Med. Chem 2006; 14: 8533–8538.

. Pereira J.A., Oliveira I., Sousa A., Valento P., Andrade P.B., Ferreira I.C.F.R., Ferreres F., Bento A., Seabra R., Estevinho L. Walnut (Juglans regia L) leaves phenolic compounds, antimicrobial activity and antioxidant potential of different cultivars. Food Chem. Toxicol 2007; 45: 2287–2295.

. Oliveira I., Sousa A., Morais J.S., Ferreira I.C.F.R., Bento A., Estevinho L., Pereira J.A. Chemical composition, and antioxidant and antimicrobial activities of three hazelnut (Corylus avellana L.) cultivars. Food Chem. Toxicol 2008; 46:1801–7.

. Negi PS, Jayaprakasha GK, Jena BS. Antioxidant and antimutagenic activities of pomegranate peel extracts. Food Chem 2003; 80: 393–7.

. Bhunia AK. Bacillus cereus and Bacillus anthracis In: Bhunia, A.K. (Ed.), Foodborne Microbial Pathogens: Mechanisms and Pathogenesis. Springer, New York, USA 2008; 135–147.

. Cushnie TPT, Lamb AJ. Antimicrobial activity of flavonoids. Int. J. Antimicrob. Agents 2005; 26: 343–56.


  • There are currently no refbacks.

Copyright (c)

                AR Journals

Street 1st, Gaytri Vihar, Pinto Park, Gwalior, M.P. India

     (Design) 2009-2021


Follow @arjournals on Twitter