Evaluation of Hepatoprotective and In-Vivo Antioxidant Activity of Tamarindus indica L. Seeds Extracts in Streptozotocin Induced Diabetic Rats

Bibekananda Meher, Deepak Das


Hydroalcoholic and aqueous extract of Tamarindus indica seeds was evaluated for hepatoprotective and antioxidant activity in Streptozotocin induced diabetic rats. Diabetes induced hepatic damage was evaluated by the serum markers such as SGOT, SGPT, ALP, Bilirubin. Further the effect of HAETIS and AETIS on oxidative stress was determined by the markers of oxidative stress: Lipid peroxidation (LPO), Determination of Super Oxide Dismutase (SOD), Determination of Catalase (CAT), glutathione (GSH) were estimated in liver tissue. HAETIS and AETIS at a dose level of  100 mg/kg and 200 mg/kg produce significant (P< 0.05) hepatoprotection by decreasing the activity of serum enzymes, biliribin and lipid perioxidation, while they significantly increase the level of Glutathione, Super Oxide Dismutase and catalase in a dose dependant manner. Histo-pathological studies supports the above finding results indicate that hydroalcoholic extracts of Tamarindus indica at 200 mg/kg showed more significant hepatic protection as compared to other extracts.


Tamarindus indica, Streptozotocin, Diabetes, hepatoprotective effect, antioxidant

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. Kokate CK, Purohit AP, Gokhale. Text book of Pharmacognosy 45th Edn. Published by : Nirali prakashan, Pune ,India , 2010,pp-1

. Satya Narayana U, Chakrapani U . Text book of Biochemistry 30th Edn Pub by :Book and allied (P) Ltd,2011,Page : 757.

. 3 Morgan K. (2002) Medicine of the Gods: Basic Principles of Ayurvedic Medicine [http://www.compulink.co.uk/~mandrake/ayurveda.htm.

. Halliwell B. Antioxidants, the basics – what they are and how to evaluate them. Adv. Pharmacol. 1997, 38, 3–20.

. Basu K, Basu LM, Indian medicinal plants, Vol-II, 2nd Edn Pub by : Bisen Sing ,2006,887-889.

. Komutarin, T, Azadi, S, Butterworth L , Keil D, Chitsomboon B, Suttajit M, Meade BJ . Extract of the seed coat of Tamarindus indica inhibits nitric oxide production by murine macrophages in vitro and in vivo. Food and Chemical Toxicology 200,42, 649–658.

. Iyer SR. Tamarindus indica L. Indian Medicinal Plants, vol. V. Pub : Orient Longman Limited, Madras, 1995 ,pp. 235–236 .

. Maiti R, Jana D, Das UK, Ghosh D. Antidiabetic effect of aqueous extract of seed of Tamarindus indica in streptozotocin-induced diabetic rats. Journal of Ethnopharmacology 2004, 92, 85–91.

. Joyeux M, Mortier F, Flurentin J. Screening of antiradical, antilipoperoxidant and hepatotoprotective effects of nine plant extracts used in Caribbean folk medicine. Phytotherapy Research 1995,9, 228–230.

. Tsuda T, Watanable M, Ohshima K, Yamamoto A, Kawakishi S, Osawa T. Antioxidative components isolated from the seed of tamarind (Tamarindus indica L.). Journal of Agricultural and Food Chemistry199, 42, 2671–2674.

. Rimbau V, Cerdan C, Vila R, Iglesia J, Antiinflammatory activity of some extracts from plants used in traditional medicines of North-African Countries (II). Phytotherapy Research 1999, 13, 128–132.

. Ramos A, Visozo A, Piloto J, Garcia A, Rodriguez, CA, Ribeiro, R, Screening of antimutagenicity via antioxidant activity in Cuban medicinal plants. Journal of Ethnopharmacology 2003,87, 241–246.

. Can A , Nuriye A, Ozsoy N, Sehnaz B , Arda BP, Yanardag R , and Okyar A Effect of Aloe vera Leaf Gel and Pulp Extracts on the Liver in Type-II Diabetic Rat Models Biol. Pharm. Bull.2004, 27(5) 694-698 .

. Khandelwal KR. Practical Pharmacognosy Techniques and Experiments. 9th ed., New Delhi, Pub by : Nirali Prakashan ,2002 , 149-157

. OECD Guideline For The Testing of Chemicals: Acute oral toxicity-Acute Toxic Class Method. 2001, 425.

. Shankar PK, Kumar V, Rao N, Evaluation of antidiabetic activity of ginkgo biloba in Streptozotocin induced diabetic rats, Iranian journal of pharmacology & therapeutics 2005, 4, 16-19.

. Retimen S, Frankel SA. Colorimetric method for determination of serum glutamic oxaloacetic and glutamic pyruvate transaminases. Am J Clin Pathol 1957; 28: 56-63.

. King EJ, Armstrong AR. A convenient method for determining of Serum and bile phosphatase activity. J Canad. Med. Assoc 1934; 31: 376-381.

. Malloy HT, Evelyn KA. The determination of bilirubin with the photometric colorimeter. J Biol Chem 1937; 119: 481-490.

. Fraga CG, Leibovitz BE, Toppel AL. Lipid peroxidation measured as TBARS in tissue characterization and comparison with homogenates and microsomes. Free Radic Biol Med 198l,4: 155- 161.

. Kakkar P, Das B, Viswanathan PN. A modi tometric assay of superoxide dismutase. Ind J Biochem Biophys, 1984, 21: 131-132.

. Maehly AC, Chance B. Methods of Biochemical Analysis, Vol I, Pub by New York. Interscience. 1954. pp. 357-358.

. Ellman GL. Tissue sulphydryl groups. Arch Biochem Biophys 1959, 82: 70-77. 33.

. Ramachandra S , Quereshi AA , Swamy VAHM , Patil T, T. Prakash , Prabhu K, Veeran Gouda A .Hepatoprotective activity of Calotropis procera flowers against paracetamol-induced hepatic injury in rats Fitoterapia 2007,78, 451–454 .

. Halliwell B , Gutteridge JMC. Free Radicals in Biology and Medicine. Oxford University Press, Oxford.1999 .

. Ceriello A . Oxidative stress and glycemic regulation. Metabolism 2000, 49, 27–29.

. Moss DW, Butterworth PJ. Enzymology and Medicine, London, Pitman Medical, 1974, pp139.

. Rauscher FM, Sanders RA, Watkins JB. Effects of coenzyme Q10 treatment on antioxidant pathways in normal and streptozotocin-induced diabetic rats , J. Biochem. Mol. Toxicol 2001, 15, 41-46.

. Debora Maria Moreno, Luzia , Neuza Jorge . Antioxidant activity, fatty acid profile and tocopherols of Tamarindus indica L. seeds Cienc. Tecnol. Aliment., Campinas, 2011,31(2): 497-501.

. Mc Lennan SV, Heffernan S, Wright L, Rae C, Fisher E, Yue DK and Turtle JR. Changes in hepatic glutathione metabolism in diabetes. Diabetes 1991, 40: 344-349.

. Anuradha CV, Selvam R. Effect of oral methionine on tissue lipid peroxidation and antioxidants in alloxan induced diabetic rats. Journal of Nutritional Biochemistry1993, 4, 212-217.

. Winterbourn CC and Munday R. Glutathione-mediated redox cycling of alloxan. Biochem. Pharmacol 1989, 38: 271-274.

. Nakakimura H and Mizuno K. Studies on lipid peroxidation in biological system: Hyperlipoperoxidemia in mice induced by alloxan. Chem. Pharm. Bull 1980, 28: 2270-2275.

. Lu SC. Regulation of hepatic glutathione synthesis: Current concepts and controversies. FASEB J 1999, 16: 1169-1183.

. Meister A. New aspects of glutathione biochemistry and transport selective alterations of glutathione metabolism. Nutr. Rev. 1984, 42: 397-401.

. Lee SE, Ju EM and Kim JH. Free radical scavenging and antioxidant enzyme fortifying activities of extracts from smilax china roots. Expt. Mol. Med. 2001,34: 263-268 .

. Sudjaroen Y, Haubner R, Würtele. Isolation and structure elucidation of phenolic antioxidants from Tamarind seeds and pericarp Food Chem Toxicol 2005,43,(11): 1673-62 .


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