An in vitro evaluation of films prepared from gelatin – Carica papaya methanolic extract for wound healing

Mohamed Abd Elgadir Saeed, Aishah Adam


This paper is a part of research aiming at preparing biodegradable films containing gelatin and Carica papaya extract for wound healing purposes in rats. Carica papaya leaves were collected and extracted using methanol aqueous method (20% methanol: 80% water). Eleven films (F1–F11) of Carica papaya extract (0–500) and gelatin (1000 mg) were prepared and investigated for selected in vitro parameters which include pH, thickness, folding endurance, water absorption capacity, tensile strength and extract released studies. Increasing concentrations of the added Carica papaya extract dropped the pH value from 7.3 ± 0.1 to 5.2 ± 0.1. Blank gelatin film had the highest value of the thickness (22.8 ± 0.9 µm). Folding endurance increased significantly (p<0.05) in both blank and composite films. In blank films the values of the folding endurance increased from 122.7 ± 3.5 – 187.6 ± 3.5 while in composite ones the values increased from 232.0 ± 8.8 to 258.3±2.5. Tensile strength value increased with increasing the addition of Carica papaya extract significantly (p<0.05) from 100.4 ± 3.4 N/cm2 to 191.5 ± 3.6 N/cm2. In contrast, within the composite films the highest addition of Carica papaya decreased the tensile strength value significantly (p<0.05) from 332.8 ± 2.2 to 84.10 ± 6.1 N/cm2. Significant differences (p<0.05) were noticed in the values of extract release. The maximum percentage of extract release was 96.6% which was scored in formulation 7 of the films (F7) while the minimum percentage of the extract release was 69.8% which was recorded in formulation 9 of the films (F9).  In conclusion, the films contained high concentrations of Carica papaya extract gave promising in vitro results and are recommended to be used for wound healing applications.


carica papaya, wound healing, gelatin, films, tensile strength, extract release

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. Das PR, Nanda RM, Behara A, Nayak PL. Gelatin blended with nanoparticle cloisite30B (MMT) or control drug delivery of anticancer drug paclitaxel. Int Res J Biochem Bioinfo. 2011; 1(2): 035– 042.

. Tamboli V, Mishra, GP, Mitra AK. Biodegradable polymers for ocular drug delivery. Adv Ocul Drug Deliv. 2012; 65 – 86.

. Elvin CM, Vuocolo T, Brownlee AG, Sando L, Huson MG, Liyou NE, Stockwell PR, Lyons RE, Kim M, Edward GA, Johnson G, McFarland GA, Ramshaw JAM, Werkmeister JA. A highly elastic tissue sealant based on photopolymerised gelatin. Biomat. 2010; 31 (32): 8323 – 8331.

. Miyoshi M, Kawazoe T, Igawa HH, Tabata Y, Ikada Y, Suzuki S. Effects of bFGF incorporated into a gelatin sheet on wound healing. J Biomater Sci Polym Ed. 2005; 16 (7): 893 – 907.

. Chong EJ, Phan TT, Lim I J, Zhang YZ, Bay BH, Ramakrishna S, Lim CT. Evaluation of electrospun PCL/gelatin nanofibrous scaffold forwoundhealing and layered dermal. Act Bio. 2007; 3 (3): 321–330.

. Asher DM. The transmissible spongiform encephalopathy agents: concerns and responses of the United States regulatory agencies in maintaining the safety of biologics. Dev Bio Stand. 1999; 100: 103 – 118.

. Lee CH, Singla A, Lee Y. Biomedical applications of collagen. Int J Pharma. 2001; 221 (1- 2): 1 – 22.

. Olsen D, Yang C, Bodo M, Chang R, Leigh S, Baez J, Carmichael D, Perala M, Hamalainen ER, Jarvinen M, Polarek J. Recombinant collagen and gelatin for drug delivery. Adv Drug Deliv Rev. 2003; 55 (12): 1547– 1567.

. Young S, Tabata MWY, Mikos A G. Microwave– treated gelatin microspheres as drug delivery system. J Control Releas. 2004; 96 (1): 67 – 84.

. Young S, Wong M, Tabata Y, Mikos AG. Gelatin as a delivery vehicle for the controlled release of bioactive molecules. J Control Releas. 2005; 109 (1–3): 256– 274.

. Jagur– Grodzinski J. Polymeric gels and hydrogels for biomedical and pharmaceutical applications. Polym Adv Technol. 2010; 21 (1): 27– 47.

. Lai J–Y, Hsieh A–C. A gelatin– g– poly (N– isopropylacrylamide) biodegradable in situ gelling delivery system for the intracameral administration of pilocarpine. Biomat. 2012; 33 (7): 2372 – 2387.

. Digenis GA, Gold TB, Shah VP. Cross– linking of gelatin capsules and its relevance to their in vitro–in vivo performance. J Pharma Sci. 1994; 83 (7): 915 – 921.

. Ulubayram K, Aksu E, Gurhan S I, Serbetci K, Hasirci N. Cytotoxicity evaluation of gelatin sponges prepared with different cross–linking agents. J Biomat Sci Polym Edu 2002; 13 (11): 1203 – 1219.

. Bigi, A, Panzavolta, S. and Rubini, K. Relationship between triple–helix content and mechanical properties of gelatin films. Biomat 2004; 25 (25): 5675 – 5680.

. Gattás–Asfura KM, Weisman E, Andreopoulos FM, Micic M, Muller B, Sirpal S, Pham SM, Leblanc RM. Nitrocinnamate–functionalized gelatin: synthesis and “smart” hydrogel formation via photo– cross– linking. Biomacro. 2005; 6 (3): 1503 – 1509.

. Kommareddy S, Amiji M. Poly (ethanol glycol)–modified thiolated gelatin nanoparticles for glutathione–responsive intracellular DNA delivery. Nanomedicine. 2007; 3 (1): 32 – 42.

. Gómez–Guillén MC, Giménez B, López–Caballero ME, Montero MP. Functional and bioactive properties of collagen and gelatin from alternative sources: A review. Food Hydrocol. 2011; 25 (8): 1813 – 1827.

. Gurung S, Skalko–Basnet N. Wound healing properties of Carica papaya latex: In–vivo evaluation in mice burn model. J Ethnopharm, 2009; 121 (2): 338 – 341.

. Anuar NS, Zahari SS, Taib IA, Rahman MT. Effect of green and ripe Carica papaya epicarp extracts on wound healing and during pregnancy. Food Chem Tox 2008; 46 (7): 2384 – 2389.

. Hima Bindu TVL, Vidyavathi M, Kavitha K, Sastry TP, Suresh kumar RV. Preparation and evaluation of ciprofloxacin loaded chitosan– gelatin composite films for wound healing activity. Int J Drug Deliv. 2010; 2 (2): 173 – 182.

. Mahmood AA, Sidik K, Salmah I. Wound healing activity of Carica papaya L. Aqeous leaf extract in rats. Int J Molec Med Adv Sci. 2005; 1 (4): 398 – 401.

. Shuid AN, Anwar M, Yusof AA. The Effects of Carica papaya Linn. Latex on the Healing of Burn Wounds in Rats. J Sains Kesih Malay 2005; 3 (2): 39 – 47.

. Nayak SB, Pinto PL, Maharaj D. Wound healing activity of Carica papaya L. in experimentally induced diabetic rats. Indian J Exp Biol 2007; 45 (8): 739 – 743.

. Collard E, Roy S. Improved function of diabetic wound–site macrophages and accelerated wound closure in response to oral supplementation of a fermented papaya preparation. Antioxid Redox Signal. 2010; 13 (5): 599 – 606.

. Tanwar YS. Formulation and evaluation of transdermal films of salbutamol sulphate. The Dhaka Uni J Pharm Sci. 2005; 4 (2): 93 – 97.

. Dhanikula AB, Panchagnula R. Development and characterization of biodegradable chitosan films for local delivery of paclitaxel. The AAPS J. 2004; 6 (3): 88 – 99.

. Schneider LA, Korber A, Grabbe S, Dissemond J. Infuence of pH on wound–healing: a new perspective for wound– therapy. Arch Dermat Res. 2007; 298 (9): 413 – 420.

. Gethin G, Cowman S: Changes in surface pH of chronic wounds when a honey dressing was used. In Proceedings of Wounds UK Conference; 13–15 November 2006; Aberdeen.

. Roberts G, Hammad L, Creevy J, Shearman C, Mani R: Physical changes in dermal tissues around chronic venous ulcers. In 7th European Conference on Advances in Wound Management. J Europ Wound Manag Assoc. 1997; 18 – 20.

. Mercier RC, Stumpo C, Rybak MJ. Effect of growth phase and pH on the in vitro activity of a new glycopeptide, oritavancin (LY333328), against Staphylococcus aureus and Enterococcus faecium. J Antimicrob Chemother. 2002; 50 (1):19 – 24.

. Thomas LV, Wimpenny JW, Davis JG. Effect of three preservatives on the growth of Bacillus cereus, Vero cytotoxigenic Escherichia coli and Staphylococcus aureus, on plates with gradients of pH and sodium chloride concentration. Int J Food Microbiol. 1993; 17 (4): 289 – 301.

. Stewart CM, Cole MB, Legan JD, Slade L, Vandeven MH, SchaVner DW. Staphylococcus aureus growth boundaries: moving towards mechanistic predictive models based on solute– specific effects. Appl Environ Microbiol. 2002; 68 (4):1864 – 1871.

. O’Meara S, Cullum N, Majid M, Sheldon T. Systemic review of wound care management: (3) antimicrobial agents for chronic wounds; (4) diabetic foot ulceration. Health Tech Assess. 2000; 4 (21):1 – 237.

. Sezer AD, Hatipoglu F, Cevher E, Oğurtan Z, Bas AL, Akbuğa J. Chitosan film containing fucoidan as a wound dressing for dermal burn healing: Preparation and in vitro/in vivo evaluation. AAPS Pharm Sci Tech. 2007; 8 (2): E94 – E101.

. Geil RD, Senkevich JJ, Rogers BR. Method for measuring solvent permeation through polymer film on porous dielectric films. J Vac Sci Tech. 2009; 27 (4): 1825 - 1828.

. Pereira R, Carvalho A, Vaz DC, Gil MH, Mendes A, Bártolo P. Development of novel alginate based hydrogel films for wound healing applications. Int J Bio Macromol. 2013; 52: 221 – 230.

. Su CH, Sun CS, Juan SW, Hu CH, Ke WT, Sheu MT. Fungal mycelia as the source of chitin and polysaccharides and their application as skin substitutes. Biomat 1997; 18 (17): 1169 – 1174.

. Nagwa F, Hanan E, Mina T. Implantable Biodegradable Sponges: Effect of Inter polymer Complex formation of Chitosan–gelatin on the release Behaviour of Tramadol HCl. D Dev and Ind Pharm. 2007; 33:7 – 17.

. Khan TA, Peh KK, Ch'ng HS. Mechanical, Bioadhesive Strength and Biological Evaluations of Chitosan films for Wound Dressing. J Pharm Pharmaceut Sci. 2000; 3 (3): 303 – 311.


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