An in vitro evaluation of films prepared from gelatin – Carica papaya methanolic extract for wound healing

Mohamed Abd Elgadir Saeed, Aishah Adam

Abstract


This paper is a part of research aiming at preparing biodegradable films containing gelatin and Carica papaya extract for wound healing purposes in rats. Carica papaya leaves were collected and extracted using methanol aqueous method (20% methanol: 80% water). Eleven films (F1–F11) of Carica papaya extract (0–500) and gelatin (1000 mg) were prepared and investigated for selected in vitro parameters which include pH, thickness, folding endurance, water absorption capacity, tensile strength and extract released studies. Increasing concentrations of the added Carica papaya extract dropped the pH value from 7.3 ± 0.1 to 5.2 ± 0.1. Blank gelatin film had the highest value of the thickness (22.8 ± 0.9 µm). Folding endurance increased significantly (p<0.05) in both blank and composite films. In blank films the values of the folding endurance increased from 122.7 ± 3.5 – 187.6 ± 3.5 while in composite ones the values increased from 232.0 ± 8.8 to 258.3±2.5. Tensile strength value increased with increasing the addition of Carica papaya extract significantly (p<0.05) from 100.4 ± 3.4 N/cm2 to 191.5 ± 3.6 N/cm2. In contrast, within the composite films the highest addition of Carica papaya decreased the tensile strength value significantly (p<0.05) from 332.8 ± 2.2 to 84.10 ± 6.1 N/cm2. Significant differences (p<0.05) were noticed in the values of extract release. The maximum percentage of extract release was 96.6% which was scored in formulation 7 of the films (F7) while the minimum percentage of the extract release was 69.8% which was recorded in formulation 9 of the films (F9).  In conclusion, the films contained high concentrations of Carica papaya extract gave promising in vitro results and are recommended to be used for wound healing applications.


Keywords


carica papaya, wound healing, gelatin, films, tensile strength, extract release

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References


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