Influence of cultural parameters on antimicrobial activity of endophytic Streptomyces sp. Cr 12 isolated from Catharanthes roseus leaves

Abdul Kafur, Anisa Basheer Khan

Abstract


Streptomyces sp. (Cr 12) was isolated from Catharanthes roseus leaves during the screening of endophytic actinomycetes for bioactive metabolites. Cell growth as well as antimicrobial metabolite production was studied in different culture media. Secondary metabolites from the strain were active against bacteria - Bacillus subtilis, Staphylococcus aureus and Proteus vulgaris as well as fungi - Botrytis cinerea, Curvularia lunata and Rhizoctonia solani. Production of bioactive metabolites by the strain was high in ISP2 broth as compared to other tested media in still conditions. The influence of different cultural parameters on antibiotic biosynthesis by Streptomyces Cr 12 was studied which showed highest antimicrobial activity at pH 7 in 28oC after 16 days of growth.


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