Influence of cultural parameters on antimicrobial activity of endophytic Streptomyces sp. Cr 12 isolated from Catharanthes roseus leaves

Abdul Kafur, Anisa Basheer Khan


Streptomyces sp. (Cr 12) was isolated from Catharanthes roseus leaves during the screening of endophytic actinomycetes for bioactive metabolites. Cell growth as well as antimicrobial metabolite production was studied in different culture media. Secondary metabolites from the strain were active against bacteria - Bacillus subtilis, Staphylococcus aureus and Proteus vulgaris as well as fungi - Botrytis cinerea, Curvularia lunata and Rhizoctonia solani. Production of bioactive metabolites by the strain was high in ISP2 broth as compared to other tested media in still conditions. The influence of different cultural parameters on antibiotic biosynthesis by Streptomyces Cr 12 was studied which showed highest antimicrobial activity at pH 7 in 28oC after 16 days of growth.

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Arnold A, Mejia L, Kyllo D, Rojas E, Maynard Z, Robbins N, Herre E. Fungal endophytes limit pathogen damage in a tropical tree. Proc Natl Acad Sci USA 2003; 100: 15649–15654.

Arai T. Actinomycetes: The Boundary Microorganisms. Toppan Co. Ltd., Singapore; 1976.

Balagurunathan R, Subramanian. Studies on marine Streptomyces nigrifaciens. I-taxonomy and standardization of antibiotic production. Cienc Mar 1993; 19: 435-443.

Berdy J. Bioactive microbial metabolites. J. Antibiot 2005; 58: 1-26.

Glazebrook MA, Vining LC, White RL, Smith KC, Chedrawy EG. Nutrient effects on growth and production of 5-hydroxy-4-oxonorvaline by Streptomyces akiyoshiensis. Can J Microbiol 1993; 39: 536-542.

Goodfellow M, O’Donnell AG. Search and discovery of industrially-significant actinomycetes. Proceedings of the 44th Symposium on Society for General Microbiology, (SCGM’89), Cambridge University Press: Cambridge 1989; 343-83.

Gramer A. Antibiotic Sensitivity and Assay Test. In: Collins CH, Lyne PM (ed) Microbiological Methods. Butterworth and Co, London 1976. p: 235.

Gupte TE, Naik SR. Optimization of nutritional requirements and process control parameters for the production of HA-2-91, a new tetraene polyene antibiotic. Hindustan Antibiot Bull 1998; 40: 5-13.

Krassilnikov NA. Intra-Strain and Intra-sp. Antagonism among Microorganisms. Doklady Akad Nauk SSSR 1960; 77: 117-119, 725-728.

Kuster E, Williams ST. Selection of media for isolation of streptomycetes. Nature (London) 1964; 202: 928-929.

Magarney NA, Keller JM, Bernan V, Dworkin M, Sherman DH. Isolation and characterization of novel marine-derived actinomycete taxa rich in bioactive metabolites. Applied Env Microbiol 2004; 70: 7520-7529.

Mincer TJ, Jensen PR, Kauffnan CA, Fenical W. Wide spread and persistent populations of a major new marine actinomycete taxon in ocean sediments. Applied Environ Microbiol 2002; 68: 5005-5011.

Okamoto-Hosaya Y, Okamoto S, Ochi K. Development of antibiotic-overproducing strains by site-directed mutagenesis of the rps1. gene in Streptomyces lividans. J Appl Environ Microbiol 2003; 69: 4256-4259.

Paul AK, Banerjee AK. Determination of optimum conditions for antibiotic production by Streptomyces galbus. Folia Microbiol (Praha) 1983; 28: 397-405.

Porter JK, Bacon CW, Robbins JD. Lysergic acid amide derivatives from Balansia epichloe and Balansia claviceps (Clavicipitaceae). J Nat Prod 1979; 42: 309–314.

Redman RS, Sheehan KB, Stout RG, Rodriguez RJ, Henson JM. Thermotolerance generated by plant/fungal symbiosis. Science 2002; 298: 1581.

Rodriguez R, Henson J, Van Volkenburgh E, Hoy M, Wright L, Beckwith F, Kim Y, Redman R. Stress tolerance in plants via habitat-adapted symbiosis. International Society for Microbial Ecology Journal 2008; 4: 404–416.

Salama AM, Mansour FA, Kamel Z. Studies on Blue-Pigmented Streptomyces Isolated from Egyptian Soil. Egypt J Bot 1980; 23: 1.

Strobel G, Daisy B. Bioprospecting for microbial endophytes and their natural products. Microbiol Mol Biol 2003; 67: 491-502.

Sujatha P, Bapi Raju KV, Ramana T. Studies on a new marine streptomycete BT-408 producing polyketide antibiotic SBR-22 effective against methicillin resistant Staphylococcus aureus. Microbiol Res 2005; 160(2): 119-26.

Watve MG, Tichoo R, Jog MM, Bhole BD. How many antibiotics are produced by the genus Streptomyces. Arch Microbiol 2001; 176: 386-90.

Wise R. The worldwide threat of antimicrobial resistance. Curr Sci 2008; 95: 181-187.

Yang SS, Wang JY. Protease and Amylase Biosynthesis of Streptomyces rimosus in Submerged and Solid State Cultivations. Bot Bull Acad Sin 1999; 40: 259-265.

Yang X, Strobel G, Stierle A, Hess WM, Lee J, Clardy J. A fungal endophyte-tree relationship; Phoma sp. In Taxus wallichana. Plant Sci 1994; 102: 1-9.


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