Retracted-Glutaraldehyde-Crosslinked Chitosan-Pectin Nanoparticles as a Potential Carrier for Curcumin Delivery and Its In Vitro Release Study

Grasianto ., Siswanta ., Mudasir ., Yosie Andriani

Abstract


Curcumin or diferuloylmethane as a naturally derived substance from  herbal remedy and a good potential to posses many diseases are widely reported.  However, Curcumin's poor aqueous solubility, low bioavalaibilty, and rapid degradation limiting clinical applicability.  Nanoparticle encapsulation solves this problem and enables extended topical delivery of curcumin. There were several in vitro and invivo study of curcumin encapsulation, but different nano carriers were used for curcumin delivery, such as palmitat, formaldehyde, sodium tripolyphosphate and genipin croslinkings. Using a Glutaraldehyde-crosslinked chitosan-pectin nanoparticle for curcumin encapsulation to Increase the curcumin bioavailability in vitro was firstly described. The prepared nanoparticles were characterized by fourier transform infrared spectroscopy (FTIR), Transmission electron micrographs (TEM), Scanning electron microscopy (SEM) and X-ray diffraction (XRD). In vitro released kinetics study was adapted from Korsmeyer-peppas model. FTIR spectra revealed that nanoparticles were formed due to interaction between the carboxyl group of pectin and the prototonated amino groups of chitosan. Characterization of encapsulated curcumin using TEM  was showed spherical morphology with size in around 40 nm. SEM revealed that no morphology changed between nanoparticles before and after the curcumin release, however the element composition of nanoparticles release has changed. Moreover, XRD analysis revealed the amorphous nature of the encapsulated curcumin. It was influenced by the concentration of polymer matrixs and glutaraldehyde. In vitro released kinetics study showed that the curcumin release from nanoparticle system tend to follow Korsmeyer-peppas model which had initial burst release followed by a steady state release. The  release of curcumin was influenced by the concentration of polymer matrixs and glutaraldehyde. The present study concluded that glutaraldehyde-crosslinked chitosan-pectin nanoparticles are promising carrier for effective delivery of curcumin. Thus, curcumin as a potential drug to cure many diseases could be explored effectively for clinical applicability.


Keywords


: nanoparticles; curcumin; glutaraldehyde-crosslinked; chitosan-pectin; kinetic study

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