Preparation and in vivo evaluation of SMEDDS containing Nevirapine for bioavailability improvement

V Vijay Kumar, J Raju, DVRN Bhikshapathi, K Naga Laxmi

Abstract


Nevirapine has been formulated in lipid-based system, a Self Emulsifying Drug Delivery System (SEDDS) to target the drug to lymphoid organs where HIV-1 virus resides in large population. Nevirapine SEDDS were formulated for enhancement of solubility, dissolution rate and oral bioavailability of model drug Nevirapine. Fourteen formulations were prepared using different oils, surfactants and co-surfactants. A pseudo ternary phase diagram was constructed to identify the self-micro emulsification region. Further, the resultant formulations were investigated for clarity, phase separation, drug content, % transmittance, globule size, freeze-thaw stability and in vitro dissolution studies. On the basis of dissolution profile and other above mentioned studies, F4 was found to be the best formulation of Nevirapine SEDDS which contains Capryol 90 (Oil), Tween 80 and PEG 600 as surfactant co-surfactant respectively. In vivo studies revealed that the oral bioavailability of Nevirapine from SEDDS was 2-fold higher compared to that of pure Nevirapine suspension in rats, suggesting a significant increase in oral bioavailability of Nevirapine from SEDDS formulation. The higher bioavailability might be due to the enhanced solubility of Nevirapine by SEDDS formulation.

Keywords


Nevirapine, SEDDS, Capryol 90, Pharmacokinetics, Bioavailability studies,

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References


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