Miconazole Nitrate based cubosome hydrogels for topical application

Maha K A Khalifa

Abstract


Cubosomes are discrete, sub-micron, nano-structured particles of bicontinuous cubic liquid crystalline phase. Cubosomes consist of honeycombed (cavernous) structures separating two internal aqueous channels and a large interfacial area. They are the biocompatible novel approach for the drug delivery system. The controlled release application of these nanoparticles is of a great significance in cosmeceutical and pharmaceutical fields. The present study is concerned with the design and evaluation of a novel nano-particulate system; cubosomes, loaded with miconazole nitrate (MN); which is used as antifungal agent. Cubosome dispersions were formulated by emulsification technique using different concentrations of a lipid phase monoolein and the nonionic surfactant, Poloxamer 407. The prepared cubosomal dispersions were characterized regarding dimensional distribution, particle size, and in vitro drug release. The optimum formulae were incorporated in a CMC or HPMC based hydrogels, to form cubosomal hydrogels (cubogels). The cubogels were characterized regarding in vitro release of (MN), viscosity and pH. A comparison between the cubogels and a commercially available product, Miconaz® cream, was carried out to judge their efficacy. The drug release from the commercial preparation was lower than all the prepared nano-emulsion based gel formulations. G1 and G8 showed highest drug release percent (100%) after 8 hours, in contrast the marketed formulation released (44.8%) of the drug after 8 hrs. The in vitro Miconazole nitrate release data were fitted to Korsmeyerpeppa's release model. The formulation exhibited non-fickian transport with zero order kinetics. Formulae G1, G8 and G10, that showed both small droplet size and highest extent of drug release, were microbiologically evaluated against Candida albicans (C. albicans) using agar cup diffusion method. The selected formulae showed superior antimycotic activity compared to the commercially available formulation.

Keywords


Cubosome, Miconazole, Candida albicans, Cubogel, Topical delivery, Nanoemulsion.

Full Text:

PDF

References


. Ramadan AA. Formulation and evaluation of bioadhesive gels containing Miconazole Nitrate. J Appl Sci Res. 2008;4(9): 1052-1065.

. Ramadan AA. Formulation and evaluation of bioadhesive vaginal suppositories containing Miconazole Nitrate, Int J Pharm Bio Sci. 2013;4(1): 455 – 472.

. Sanap GS, Mohanta GP. Solid lipid nanoparticles (SLN) – based hydrogel formulation for topical delivery of Miconazole nitrate. Int J Pharm Bio Sci. 2013;4(2): 21 – 33.

. Nasr M, Ghorab MK, Abdelazem A. In vitro and in vivo evaluation of cubosomes containing 5-fluorouracil for liver targeting, Acta Pharmaceutica Sinica B, 2015;5(1):79–88.

. Garg G, Saraf S, Saraf S. Cubosomes: An Overview, Biol Pharm Bull, 2007;30 (2) 350-353.

. Bhosale RR, Osmani1 RA, Harkare BR, Ghodake PP. Cubosomes: The inimitable nanoparticulate drug carriers. Sch Acad J Pharm, 2013;2(6):481-486.

. Thapa RK, Baskaran R, Madheswaran T, Kim JO, Yong CS, Yoo B K. In vitro release and skin permeation of tacrolimus from monoolein-based liquid crystalline nanoparticles. J Drug Del Sci Tech. 2012;22 (6) 479-484.

. Zhou Y, Guo C, Chen H, Zhang Y, Peng X, Zhu P. Determination of Sinomenine in cubosome nanoparticles by HPLC technique, Journal of Analytical Methods in Chemistry. 2015; http://dx.doi.org/10.1155/2015/931687.

. Tilekar K, Khade P, Kakade S, Kotwal S, Patil R. Cubosomes-a drug delivery system, IJPCBS. 2014;4(4): 812-824.

. Sherif S, Bendas ER, BadawyS. The design and evaluation of novel encapsulation technique for topical application of alpha lipoic acid, JAPR. 2013;4(1): 13-22.

. Morsi NM, Abdelbary GA, Ahmed MA. Silver sulfadiazine based cubosome hydrogels for topical treatment of burns: Development and in vitro/in vivo characterization, Eur J Pharm Biopharm, 2014;86(2): 178–189.

. Esposito E, Puglia C, Drechsler M, Paccamiccio L, Mariani P, Contado C, Menegatti E, Bonina F, Cortesi R. Production and characterization of cubosome: A new tool for percutaneous administration of lipophilic drugs, Proc. 5th world meeting on Pharmaceutics, biopharmaceutics and pharmaceutical technology.Geneva, 2006;27-30.

. Esposito E, Eblovi N, Rasi S, Drechsler M, Di Gregorio G, Menegatti E, Cortesi R. Lipid-based supramolecular systems for topical application: A preformulatory study, AAPS Pharm Sci. 2003; 5 (4): 62-76.

. Elosaily GH. Formulation and in vitro evaluation of Nystatin nanoemulsion-based gel for topical delivery, Journal of American Science, 2012;8(12) http://www.jofamericanscience.org.

. Marzouk MA, Ammar AA, Darwish MK, El-sayed HA. Effect of penetration enhancers on in vitro permeation of Nyststin from topical formulations, Int J Drug Dis. 2012;4 (2):153.

. Muraleedhara KK, Senthilkumar SK, Parthiban S. Formulation and evaluation of muco-adhesive thermo-sensitive pluronic lecithin organogel of antifungal agent for vaginal candidiasis, International Journal of advanced pharmaceutics. 2013;3 (2): 70-77.

. Rosen MR. Delivery System Handbook for Personal Care and Cosmetic Products: Technology, Applications, and Formulations, William Andrew Pub.,Norwich, N. 2005.

. ZhaoX. Y, Zhang J, Zheng L, Hua Li D. Studies of cubosomes as a sustained drug delivery system, J disp sci technol. 2004;25(6): 795–799.

. Tobyn MJ, Johnson JR, Dettmar PW. Factors affecting in vitro gastric mucoadhesion. Part 2: Physical properties of polymers, Eur. J.Pharm. and Biopharm.1996;42(1): 56.

.Archana A, Vijayasri K, Madhurim M, Kumar CA. Curcumin loaded nano cubosomal hydrogel: preparation, in vitro characterization and antibacterial activity, Chem Sci Trans. 2014;4(1): 75-80.

. Hashem FM, Shake DS. Ghorab MK, Nasr M, Ismail A. Formulation, characterization and clinical evaluation of microemulsion containing Clotrimazole for topical delivery. AAPS Pharm Sci Tech. 2011;12(3):879-86.

.Shahzadi I, Masood MI, Chowdhary F, Anjum AA, Nawaz MA, Maqsood I, Zaman M Q. Microemulsion formulation for topical delivery of Miconazole Nitrate. Int J Pharm Sci Rev Res,2014;24(2): 30-36.


Refbacks

  • There are currently no refbacks.




Copyright (c) 2015 Maha KA Khalifa

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

               AR Journals

18K, Street 1st, Gaytri Vihar, Pinto Park, Gwalior, M.P. India

Copyright@arjournals.org (Design) 2009-2021

 

Follow @arjournals on Twitter