Controlled delivery of Imatinib mesylate from collagen coated poly(lactic acid) microspheres: In vitro release studies

Chandra Babu A, Subha MCS, Chowdoji Rao K

Abstract


The development of injectable microspheres for controlled drug delivery to the desired site is a major challenge. We demonstrated the possibility of entrapping an anticancer drug, Imatinib mesylate, in collagen coated biodegradable poly (lactic acid) microspheres with a mean diameter of 10-20 µm. The collagen coating on polymeric matrix surfaces through various surface modification techniques was the current scenario to improve bio-integration of the polymers with the in-vivo system. Here protein adsorption principle is used and various characterization techniques like FTIR, DSC and SEM analysis are used to confirm collagen coating. The reduction in burst release of the Imatinib from the PLA microspheres further confirms its presence and role in controlled release.  This collagen coated PLA microspheres may have potential for the targeted delivery of Imatinib mesylate to treat gastrointestinal stromal tumors, chronic myeloid leukemia cancer.


Keywords


Drug Delivery

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References


Shimori K, Kawano Y, Kiyoyama S. Adsorption Equilibriya of the Organic Acids on Activated Carbon at Various Temperatures. Kagaku Kogaku Ronbunshu, 1994; 20:453-458.

Rouzes C, Leonard M, Durand A, Dellacherie E. Influence of polymeric surfactants on the properties of drug-loaded PLA nanospheres. Colloids Surf. B: Biointerf. 2003; 32:125-135.

Changnot D, Six JL, Leonard M, Bonneaux F, Vigneron C, Dekkacherie E. Physicochemical evaluation of PLA nanoparticles stabilized by water-soluble MPEO-PLA block copolymers. Colloid Interf. Sci. 2003; 268:441-447.

Freiberg S, Zhu XX. Polymer microspheres for controlled drug release. Int. J. Pharm. 2004; 282:1-18.

Lu Z, Ballinger JR, Rauth AM, Bendayen R, Wu XY. Delivery of an anticancer drug and a chemosensitizer to murine breast sarcoma by intratumoral injection of sulfopropyl dextran microspheres. J. Pharm. Pharmacol. 2003; 55:1063-1073.

Cheung RY, Rauth AW, Wu XY. In vivo efficacy and toxicity of intratumorally delivered mitomycin C and its combination with doxorubicin using microsphere formulations. Anticancer Drugs 2005; 16:423-433.

DeFail AJ, Edington HD, Mathews S, Lee WCC, Marra KG. Controlled release of bioactive doxorubicin from microspheres embedded within gelatin scaffolds. J. Biomed. Mater. Res. A 2006; 79:954-962.

David GS, Richard MS, John MG. Brit. Clinical features at diagnosis in 430 patients with chronic myeloid leukaemia seen at referral centre over a 16 year period. Brit. J. Hematology 1997; 96:111-116.

Jabbour E, Cortes JE, Giles FJ, O’Brien S, Kantarjian HM. Current and emerging treatment options in chronic myeloid leukemia. Cancer 2007; 109:2171-2181.

Yi H, Changyou G, Ying X, Yihong G, Jiacong S. Collagen-coated polylactide microspheres as chondrocyte microcarriers. Biomaterials 2005; 26:6305-6313.

Van Wachem PB, Beugelling T, Feijen J, Bantjes A, Detmers JP, Van Aken WG. Interaction of cultured human endothelial cells with polymeric surfaces of different wettabilities. Biomaterials 1985; 6:403-408.

Zhu YB, Gao CY, He T, Shen JC. Endothelium regeneration on luminal surface of polyurethane vascular scaffold modified with diamine and covalently grafted with gelatin. Biomaterials 2004; 25:423-430.

Zhu YB, Gao CY, He T, Liu XY, Shen JC. Layer-by-Layer Assembly to Modify Poly(L-lactic acid) Surface toward Improving Its Cytocompatibility to Human Endothelial Cells. Biomacromolecules 2003; 4:446-452.

Ma ZW, Gao CY, Gong YH, Ji J, Shen JC. Immobilization of Natural Macromolecules on Poly-L-Lactic Acid Membrane Surface in order to improve Its cytocompatibility. J. Biomed. Mater. Res. 2002; 63:838-847.

Raffaini G, Ganazzoli F. Understanding the Performance of Biomaterials through Molecular Modeling: Crossing the Bridge between their Intrinsic Properties and the Surface Adsorption of Proteins. Macromol. Biosci. 2007; 7:552-566.

Mallikarguna B, Madhusudhana Rao K, Siraj S, Chandra Babu A, Chowdoji Rao K, Subha MCS. Sodium Alginate/poly (ethylene oxide) blend hydrogel membranes for controlled release of Valgano cyclovir hydrochloride. Designed Monomers and Polymers 2013; 16:151-159.

Sudhakar K, Madhusudhana Rao K, Sudhakar P, Chandra Babu A, Kumara Babu P, Subha MCS, Chowdoji Rao K. Development of pH sensitive polycaprolactone-based microspheres for In-vitro release studies of Triprolidine Hydrochloride. Designed Monomers and Polymers 2014; 17:617-623.

Natarajan V, Krithica N, Madhan B, Sehgal PK. Formulation and evaluation of quercetin polycaprolactone microspheres for the treatment of rheumatoid arthritis. J. Pharm. Sci. 2011; 100:195-205.


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