Antitumor and structure antioxidant activity relationship of Colchicine on Ehrlich ascites Carcinoma (EAC) in Female Mice

Mohammed Abdalla Hussein, Sylvia A Boshra


Colchicine has been reported to play important roles in hepatoprotection, anti-inflammation in vitro anti cancer activity. The present study was initiated to evaluate antioxidant and anti-cancer effects of colchicine (10µg/mice, i.p.) in mice after subcutaneous implantation of ehrlich ascites carcinoma (EAC) for 21 days. On the 22th day, the mice were sacrificed for the estimation of tumor growth, and biochemical parameters (glucose, insulin, alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), lipid peroxides (TBARS), protein thiols (Pr-SHs), reduced glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx), total cholesterol (TC), triglycerides (TG), HDL-C, LDL-C, 17β-estradiol and progesterone). The results of this study showed that administration of colchicine and 5-Flourouracil individually for 21 days to the carcinoma induced mice demonstrated a significant (P<0.01) decrease in tumor weight and a significant (P<0.01) improvement in biochemical parameters and life span  compared to the EAC control mice. In addition, the results clearly suggest that colchicine induced antioxidant activity on experimental EAC control mice.

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. Jemal A, Siegel R, Ward E, Murray T, Xu J, Smigal C, Thun MJ.

Cancer statistics. CA Cancer J Clin 2006, 56:106-130.

. Ponder BAJ. Cancer genetics. Nature 2001, 411:336-341.

. Noda N, Wakasugi H. Cancer and oxidative stress. Journal of the Japan Medical Association. 2000, 124, 11:1571–1574.

. Brown JE, Khodr H, Hider RC, Rice-Evans C. Structural dependence of flavonoid interactions with Cu2+ ions: implications for their antioxidant properties. Biochem. J. Vol. 1998, 330: 1173-1178.

. Talib WH, Mahasneh AM. Antimicrobial, cytotoxicity and phytochemical screening of Jordanian plants used in traditional medicine. Molecules 2010, 15:1811-1824.

. Ezell SJ, Li H, Xu H, Zhang X, Gurpinar E, Zhang X, et al. Preclinical pharmacology of BA-TPQ, a novel synthetic iminoquinone anticancer agent. Mar. Drugs 2010, 8: 2129-2141.

. Calogero M. Ortopedia e Traumatologia oggi Anno XI (2) aprile. 1992.

. Bharathi.P, D.Philomina and S.Chakkarvarthi, Antimitotic effect of colchicine from six different species of Gloriosa superb in onion roots Allium cepa). J. Med. Sci. 2006, 6:420-425.

. Alali F, K.Tawaha and Rh. Qasaymch, Determination of Colchicines in Colchicum steveni and C. hierosolymitanum (colchicaceae): comparison between two analytical methods. Photochem. Anal. 2004, 15:27-29.

. Evans, D.A., S.P.Tanis and D.J.Hart. A convergent total synthesis of (±) colchicines and (±) Deacetoamidoisocolchicine. J. Am. hem. soc. 1981, 103: 5813-5821.

. Newman R, Yang J, Finlay R, Cabral F, Vourloumis D, et al. Cancer Chemotheraphy and Pharmacology, 2001, 48: 319-324.

. Ehrlich HP, Barnstein P. M~crohrbuleisn transcehrllar movement of procollagen. Nature (London) 1972, 238: 257- 264.

. Diegelman RF, Petakofslry B. Inhibition of collagen secretion from bone and cultured fibroblasts by microtubules disruptive bgs. Proc Natl Acad Sci USA 1972, 69: 892- 899.

. Hmis ED, Kram SM. Collagenases. N Engl J Med 1974, 29: 1557-1563.

. Yahuaca, Amaya A, Rojkind M, Mourelle M. Cryptic ATPase activity in plasma membranes of CC14-cirrhotic rats. Its modulation by changes in cholesterol phospholipid ratios. Lab Invest 1985, 53:54l-548.

. Mourelle M, Rojkind M, Rubalcaba B. Colchicine improves the alterations in the liver adenylate cyclasc system of cirrhotic rats. Toxicology 1981, 21: 213-220.

. Lemi S, Spagnuolo S, Conti de ViL. Effects of colchicine on rat liver plasma membranes. Biochim Biophys Acta 1980, 596:451-458.

. Asirvatham Raju, Christina AJM. Antitumor Potential of Drosera Indica L against Ehrlich Antitumor Potential of Drosera Indica L against Ehrlich Ascites Carcinoma (EAC) Tumor in Mice. Am J Pharm Tech Res 2012, 3: 955-962.

. Liliana F and Victor P. Comparative Effects of Colchicine and Silymarin on CC14-Chronic Liver Damage in Rats. Archives of Medical Research. 1997; 28: 11-17.

. Raju A, Arockiasamy Josphin MC. Modulatory effects of Drosera Indica L on EAC induced metabolic changes in mice. Molecular & Clinical Pharmacology 2013, 4(1), 59-64.

. Kuttan G, Vasudevan DM, Kuttan R. Effect of a preparation from Viscum album on tumor development in vitro and in mice. Journal of Ethnopharmacology 1990, 29: 35-41.

. Mazumder UK, Gupta M, Maiti S, Mukherjee M. (1997). Antitumor activity of Gygrophila spinosa on Ehrlich ascites carcinoma and sarcoma-180 induced mice. Indian. Journal of Experimental Biology 1997, 35: 473-477.

. Attia M, Weiss DW. Immunology of spontaneous mammary carcinomas in mice infected with mammary tumour virus. Cancer Res 1966, 26:1787–800.

. Waldhausl WK., Gasic S, Bratush-Marrain P, Nowotny P. The 75-gram oral glucose tolerance test: Effects on splanchnic metabolism of substrates and pancreatic hormone release in healthy man. Diabetologia.1983, 25: 489-495.

. Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, Turner RC. Homeostasis model assessment: insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologi.1985; 28:412-19.

. Anup M, Sandip K, Batabyal, Mrinal K, Poddar. Long- term caffeine induced inhibition of EAC cell progression in relation to gonadal hormone status. Indian J Exp Biol 2007, 45, 347-352.

. Rezvanfar MA, Sadrkhanlou RA, Ahmadi A, Shojaei-Sadee H, Rezvanfar MA, Mohammadirad A, Salehnia A, Abdollahi M. Protection of cyclophosphamide-induced toxicity in reproductive tract histology, sperm characteristics, and DNA damage by an herbal source; evidence for role of free-radical toxic stress. Hum Exp Toxicol 2008, 27, 901-910.

. Reitman S, Frankel S. A colorimetric method for the determination of serum oxaloacetic acid and glutamic pyruvic transaminases. Am. j. Clin. Pathol 1957, 28: 56 – 63.

. Kind PRN, King EJ. Estimation of plasma phosphatase by determ-ination of hydrolysed phenol with amino-antipyrine. J. Clin. Pathol 1954, 7: 322 – 326.

. Buhl SN, Jackson KY. Optimal conditions and comparison of lactate dehydrogenase catalysis of the lactate to pyruvate to lactate reactions in human serum at 25, 30 and 37 0C. Clin. Chem. 1978; 2415: 828.

. Nichans WH, Samulelson B. Formation of malondialdehyde from phospholipid arachidonate during microsomal lipid peroxidation. Eur J Biochem 1968, 6: 126-30.

. Ellman GL. Tissue sulfhydroyl groups. Arch.Biochem.Biophy 1959, 82: 70-6.

. Chanarin I. Text book of Laboratory Haematology: An Account of Laboratory techniques, Churchill Livingstone, New York PP. 1989; 107.

. Marklund S, Marklund D. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem., 1974; 47:469.

. Paglia D, Valentine W. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J. Lab. Clin. Med 1967, 70:158.

. Fossati P, Prencipe L. Serum triacylglycerols determined calorimetrically with an enzyme that produces hydrogen peroxide. Clin Chem 1982, 1: 2077-2080.

. Allain CC, Poon LS, Chan CS, Richmond W, Fu PC. Enzymatic determination of total serum cholesterol. Clin Chem 1974, 4: 470-475.

. Friedewald WT. Estimation of concentration of low-density lipoprotein cholesterol in plasma without use of the preparative ultracentrifuge. Clin Chem. 1972, 18:499-502.

. Falholt K, Falholt W, Lund B. An easy colorimetric method for routine determination of free fatty acids in plasma. Clin Chim Acta 1973, 46: 105–111.

. Mons S, Veretout F, Carlier M, Erk I, Lepault J, Trudel E, Salesse C, Ducray P, Mioskowski C, Lebeau L. The interaction between lipid derivatives of colchicine and tubulin: consequences of the interaction of the alkaloid with lipid membranes. Biochim Biophys Acta. 2000, 1468:381-95.

. SPSS. (SPSS 15, Inc., Chicago,IL, USA).2012.

. Lee KH. Novel antitumor agents from higher plants. Med Res Rev. 1999, 19:569-596.

. Ben-Chetrit E, Levy M. Colchicine; Update. Seminars in Arthritis and Rheumatism, 1998, 28:48–59.

. Chang HR, Lai CC, Lian JD, Lin CC, Wang CJ. Formation of 8-nitroguanine in blood of patients with inflammatory gouty arthritis. Clinica Chimica Acta 2005, 362:170–5.

. Aboutwerat A, Pemberton PW, Smith A, Burrows PC, McMahon RF, Jain SK, Warnes TW. Oxidant stress is a significant feature of primary biliary cirrhosis. Biochim Biophys Acta. 2003, 1637:142-50.

. Bala A, Kar B, Haldar PK, et al. Evaluation of anticancer activity of Cleome gynandraon Ehrlich’s ascites carcinoma treated mice. J Ethnopharmacol 2010, 129:131-4.

. Gupta M, Mazumder UK, Haldar PK, Chicago. Anticancer activity of Indigofera aspalathoides and Wedelia calendulaceae in Swiss albino mice. Iranian J Pharm Res 2007, 6:141-5.

. Coe EL. Correlation of glycolytic and respiratory events after addition of a small amount of glucose to Ehrlich ascites carcinoma. Cancer Res 1966, 26: 269-275.

. Nakamura W, Hosoda S. The absence of glucose in Ehrlich ascites tumor cells and fluid. Biochim.Biophys. Acta 1968, 158:212-218.

. Weber MJ. Hexose transport in normal and in Rous sarcoma virus. transformed cells. J. Blol. Chem 1973, 248: 2978-2983.

. Hatanaka M, Hanafusa H. Analysis of a functional change in membrane in the process of cell transformation by Rous sarcoma virus; alteration In the characteristics of sugar transport. Virology 1970, 4 1: 647-652.

. Dunbar JC, Walsh MF, Foa PP. Secret Ion of immune reactive insulin and glucagon in hamsters bearing a transplantable insulinoma. Diabete Metab 1976, 2: 165-169.

. Shapot, V. S. Some biochemical aspects of the relationship between the tumor and the host. Adv. Cancer Res 1972, 15: 253-286.

. Raju A, Arockiasamy JMC. Modulatory effects of Drosera Indica L on EAC induced metabolic changes in mice. Molecular & Clinical Pharmacology 2013, 4: 59-64.

. Abu-Sinna G, Esmat AM, Al-Zahaby S, Soliman NA, Ibrahim TM. Fractionation and characterization of Cerastes snake venom and the antitumor action of its lethal and non-lethal fractions. Toxicon 2003, 42: 207-215.

. Hussein MA. Synthesis, anti-inflammatory, and structure antioxidant activity relationship of novel 4-quinazoline. Med Chem Res 2013, 22:4641–4653.

. Wagner H, Geyer B, Yoshinobu K, Govind SR. Coumestan as the main active principles of liver drugs Eclipta alba and Wedelica calendulaceae. Planta Med 1986, 5: 370-2.

. Anup M, Sandip K, Mrinal K. Long- term caffeine induced inhibition of EAC cell progression in relation to gonadal hormone status. Indian J Exp Biol 45, 347-352.


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