Cytotoxic potential of Cissus quandrangularis on Allium cepa root meristem

Rubeena Mattummal, John E Thoppil

DOI: http://dx.doi.org/10.5138/09761055.2212

Abstract


Apoptosis is a developmental and regulated process which may occur as a result of the cytogenetic changes in the cell. Cissus quandrangularis is a xerophytic plant that  belongs to the family Vitaceae, commonly distributed throughout the hotter parts of India and Sri Lanka. An attempt had been made to explore the apoptosis-mediated cytotoxic potential of C. quadrangularis on Allium cepa. Cytogenetic activities of the plant were revealed using DAPI staining and in situ visualization of cell death was carried out with the help of Evans blue staining. The presence  of clastogenic (nuclear lesions, chromosome bridges, chromosome breakage and pulverization of chromosomes) and non-clastogenic abberations (ball metaphase, stellate metaphase, chromosome clumping and early movement of chromosomes) observed under fluorescent microscope shows the cytotoxic effect of the plant extract. The quantification of cell death was measured by reading absorbance at 600 nm using the spectrophotometer.  The cytotoxic effects on Allium root tips shows the apoptotic potential of the plant extract and the present study enlightens the anticancer potential of the plant.


Full Text:

PDF

References


[1] Elmore S. -. Apoptosis: A Review of Programmed Cell Death Toxicol Pathol. 2007;35(4):495–516.

[2] Golstein P, Kroemer G. Cell death by necrosis: towards a molecular definition. Trends Biochem Sci. 2007;32(1):37–43.

[3] Kerr JF, Winterford CM, Harmon BV. Apoptosis: Itssignificance in cancer and cancer therapy. cancer. 1994;73:2013–2026.

[4] Kumar SP, Soni, Saraf MN. In-vitro tocolytic activity of Sarcostemma brevistigma Wight. Indian J Pharm Sci. 2006;68:190–194.

[5] Chauhan L, Saxena PN, Gupta SK. Cytogenetic effect of cypermethrin and fenvalerate on the root meristem cells of Allium cepa. Environ Exp Bot. 1999;42:181–189.

[6] Nadkarni AK. Indian Materia Medica, Popular prakasan. Bombay. 1954;3:1284–1286.

[7] Chopra RN, Sl N, Ic C. Glossary of Indian Medicinal Plants, National Institute of Science and Communication. New Delhi: CSIR; 1986.

[8] Baker, Mock CJ, NM. An improved method for monitoring cell death in cell suspension and leaf disc assays using Evans blue. Plant Cell Tissue Organ Cult. 1994;39:7–12.

[9] El-Ghameri, Elnahas AA, Mansour AI, MM. The action of atrazine herbicide as an inhibitor of cell division on chromosomes and nucleic acids content in root meristems of Allium cepa and Vicia faba. Cytologia. 2000;55:209–215.

[10] Fenech M. Biomarkers of genetic damage for cancer epidemiology. Toxicology. 2002;p. 411–416.

[11] Fernandes T, Dec M, Ma MM. Mechanism of micronuclei formation in polyploidizated cells of Allium cepa exposed to tri-fluralin herbicide. Pestic. Biochem Physiol. 2007;88:252–259.

[12] Kirsch-Volders, Elhajouji M, E, CP, Hum-Melen V. The in vitro micronucleus test: a multi-end-point assay to detect simultaneously mitotic delay, apoptosis, chromosome breakage, chromosome loss and non-disjunction. Mutat
Res. 1997;392:19–30.

[13] Panda KK, Achary V, Krishnaveni R, Padhi BK, Sarangi SN, Sahu SN, et al. In vitro biosynthesis and genotoxicity bioas- say of silver nanoparticles using plants. Toxicol In Vitro. 2011;25(5):1097–1105.

[14] Achary V, Panda JS, KK, Bb P. Aluminium induced oxidative stress and DNA damage in root cells of Allium cepa L. Ecotoxicol Environ Saf. 2008;70(2):300–310.

[15] Sehgal R, Roy S, Vl K. Evaluation of cytotoxic potential of latex of Calotropis procera and Podophyllotoxin in Allum cepa root model. Biocell. 2006;30(1):9–13.

[16] Prashar A, Locke IC, Evans CS. Cytotoxicity of clove (Syzygium aromaticum) oil and its major components to human skin cells. Cell Proliferation. 2006;39(4):241–248.

[17] Bhagyanathan NK, Thoppil JE. Pre-apoptotic activity of aqueous extracts of Cynanchum sarcomedium Meve & Liede on cells of Allium cepa and human erythrocytes. Protoplasma. 2016;253(6):1433–1438.


Refbacks

';



Copyright (c) 2018 Rubeena Mattummal, Dr John E Thoppil

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

              AR Journals

18K, Street 1st, Gaytri Vihar, Pinto Park, Gwalior, M.P. India

       Copyright@arjournals.org (Design) 2009-2021

 

Follow @arjournals on Twitter