Retracted:In vitro regeneration of high yielding cultivars of rice (Oryza sativa L.)

Dipti Verma, Awanish Kumar, Alok Shukla



In vitro regeneration in rice (Oryza sativa L.) with high reproducibility is still in need for their further exploitation in transgenic development or selection against different stress.  The present study was undertaken to evaluate the in vitro performance of high yielding cultivars of Govind, Jaya and Pusa Basmati-1, using  most suitable concentration of growth regulators i.e., 2,4- dichlorophenoxyacetic acid (2,4-D), Indole-3- acetic acid (IAA), benzyl amino purine (BAP) and kinetin (Kn) for callus induction, proliferation and regeneration in mature embryo of rice. Maximum callus induction was obtained in rice cultivar Govind on MS medium supplemented with 13.5 mM 2,4-D. A good callus proliferation was obtained in hormone free MS media. Excellent regeneration was observed in rice cultivar Pusa Basmati-1 in MS medium supplemented with 2.2mM BAP. Multiple shoots e.g., 10 in rice cultivar Pusa Basmati-1 (PB-1) were regenerated from the single callus. In vitro developed plants were acclimatized gradually and their performance in pots was satisfactory.

Key words: Indica rice, Callus culture, mature embryo, regeneration.


Indica rice, Callus culture, mature embryo, regeneration.


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