Spectrophotometric Determination of Tranexamic Acid in Bulk and Dosage Forms Using Ascorbic Acid

Magdi Awadalla Mohamed, Elrasheed Ahmed Gadkariem, Maha Abdalla Ahmed Jabbir

Abstract


A simple spectrophotometric method for the determination of tranexamic acid (TA) in pure form and pharmaceutical preparations was developed.  The method is based on coupling tranexamic acid with ascorbic acid (AA) in dimethyl sulfoxide (DMSO) to produce a colored product which absorbs maximally at two wavelengths (λmax) 390nm & 530nm. The molar ratio of tranexamic acid: ascorbic acid is 1:2. Beer’s law is obeyed in the concentration range 10-25µg/ml of tranexamic acid with molar absorbtivity of 2.4×103 Lmol-1cm-1max390nm) and 0.73×103 L mol-1cm-1 max530nm). Different experimental parameters affecting the development and stability of the color were   studied and optimized. The proposed method was validated against an adopted B.P formal titration method.  The mean percentage recovery obtained for the assay of commercial capsules was found to be 99.77±1.02 (λmax390nm) and 101.01±0.97 (λmax530nm).

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