Antioxidant and Antimicrobial activities of Endophytic Fungi isolated from Sesbania grandiflora (L.) Pers.

Pannapa powthong, Acharawan Thongmee, Pattra Suntornthiticharoen


Twenty eight different endophytic fungi were isolated from Sesbania grandiflora (L.) Pers. These endophytic fungal extracts were prepared, using ethyl acetate and evaluated for their phytochemical constituents in vitro. The antibacterial activity of crude extract was done by using broth microdilution and exhibited significant antimicrobial activity against an array of plant pathogenic bacteria included Xanthomonas axonopodis pv. citri,        Xanthomonas axonopodis pv. glycines, Xanthomonas campestris pv.campestris and Acidovorax avenae subsp. Avenae range from 125-2000 μg/mL. The total antioxidant capacity was evaluated by using 2, 2-diphenyl-1-picryl-hydrazyl (DPPH) assay ranged from 2.10 x 10-3 to 4.84 x 108 µg /mg extract. The total phenolic content of the fungal extracts was determined by using Folin-Ciocalteu procedures which ranged from 0 to 1730.78 µg gallic acid/mg extract. This is the first report of Sesbania grandiflora (L.) Pers. producing endophytes which have antimicrobial and antioxidant activities. This investigation reveals that the metabolites produced by a variety of endophytic fungi from Sesbania grandiflora (L.) Pers. can be a potential source of novel natural antimicrobials and antioxidants agents.


Antimicrobials, Antioxidant, DPPH, phenol, Sesbania grandiflora (L.) Pers..

Full Text:



Anderson D. (1996) Antioxidant defences against reactive oxygen species causing genetic and other damage.Mutat. Res. 350, 103-108

Devdatta, Appanna. (1954). Nutritive value of Indian foods .Indian Acad. Sci. 398: 297

Ding T., Jiang T., Zhao J., et al. (2010) Evaluation of antimicrobial activity of endophytic fungi from Camptotheca acuminata (Nyssaceae). Genetics and Molecular Research 9: 2104 -2012

Harper JK, Ford EJ, Arif AM, et al. (2003) Pestacin: a 1,3 –dihydro isobenzofuran from Pestalotiopsis microspora possessing antioxidant and antimycotic activities Tetrahedron 59:2471-2476

Gould GW. (1995) Biodeterioration of foods and an overview of preservation in the food and dairy industriesInt. Biodeter. Biodegr. 36:267-277

Guo B. (2000) Cytonic acids A & B: novel tridepside inhibitors of hCMV protease from the endophytic fungus Cytonaema species Journal of Natural Product 63: 602-604

Gutteridge RC (1994) Forage tree legumes in tropical agriculture In: Tropical Tree Legumes in Tropical Agriculture Edited by Gutteridge, R.C. and Shelton, H.M. (ors). CAB International, UK, 49

Jao CL, and Ko WC (2002) 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging by protein hydrolyzates from tuna cooking juice. Fisheries Science 68: 430-435

Jiang DF, Ma P., Wang X.H., et al. (1995) The studies of fungal population and relationship between fungi and forming of dragon's blood resin in Dracaena cochinchinensis. Acta Botan Ynnnanica 17: 79-82

Jin H., Geng YC, Yu ZY et al. (2009) Lead optimization and anti-plant pathogenic fungi activities of daphneolone analogues from Stellera chamaejasme L. Pestic. Biochem. Phys. 93:133-137

Mendoza VB (1980) Katurai: a plant of many uses. Canopy International 12-13

Liu CH, Chen X., Liu TT, et al. (2007) Study of the antifungal activity of Acinetobacter baumannii LCH001 in vitro and identification of its antifungal components. Appl Microbiol. Biotechnol. 76, 459-466

National Committee for Clinical Laboratory Standards (NCCLS). (2002) In 12th informational supplement M-100-S12. Wayne, Pa: NCCLS

National Committee for Clinical Laboratory Standards. (2002) second edition. In Approved standard M27-A2. Wayne, Pa:NCCLS

National Committee for Clinical Laboratory Standards. (2002) In Approved standard. NCCLS document M38-A.Wayne, Pa: NCCLS;

Padmaja M., Sravanthi M., Hemalatha KPJ. (2011) Evaluation of antioxidant activity of two Indian medicinal plants..J .Phytol. 3: 86-91

Phongpaichit S., Nikom J., Rungjindamai N et al. (2007) Biological activities of extracts from endophytic fungi isolated from garcinia plants. Fems Immunol. Med. Mic. 51:517-525

Petrini O (1991) Fungal endophytes of tree leves In: Microbial Ecology of Leaves (Andrews JA and Hirano SS, eds.).Spring-Verlag, New York, 179-191

Reische DW, Lillard DA and Eitenmiller RR (1998) Antioxidants. In: Food Lipids: Chemistry, Nutrition, and Biotechnology (Akoh CC and Min DB, eds.). Marcel Dekker, New York, 423

Scazzocchio F., Cometa MF, Tomassini L, (2001) Antibacterial Activity of Hydrastis canadensis Extract and its Major Isolated Alkaloids. Planta Med. 67: 561-564

Shyamala gowri S. and Vasantha K. (2010) Free radical scavenging and antioxidant activity of leaves from Agathi (Sesbania grandiflora) (L.) Pers.Am. Eur. J. Sci. Res.5:114

Singleton VL, Orthofer R. and Lamuela-Raventos RM (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent..Methods in Enzymology. 29:152-178

Stierle A., Strobel G. ,Stierle D. (1993) Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of Pacific Yew.Science 260: 214

Strobel G., Yang X., Sears J., et al. (1996) Taxol from Pestalotiopsis micrspora, an endophytic fungus of Taxus wallichiana. Microbiology 142: 435-440

Strobel GA (2002) Rainforest Endophytes and Bioactive Products., Crit. Rev. Biotechnol. 22: 315

Tan R., Zou WX, (2001) Endophytes: a rich source of functional metabolites. Natural Product Reports 18: 448-459

Tejesvi MV, Kini KR, Prakash HS, V et al. (2008) Antioxidant, antihypertensive, and antibacterial properties of endophytic Pestalotiopsis species from medicinal plants.Can J Microbiol. 54:769-780

Tian XL, Cao LX, Tan HM, et al. (2004) Studyon the communities of en-dophytic fungi and endophytic actinomycetes from rice and theirantipathogenic activities in vitro. World J. Microbiol. Biotechnol. 20:303-309

Vipin K., Arun KG, and Rajesh G. (2011) Pharmacognostical investigation on Sesbania grandiflora (L.) Pers.. Int J Pharm Sciences and Res 2: 1069-1072

Wu CR, Huang MY, Lin YT et al. (2007) Antioxidant properties of cortex fraxini and its simple coumarins. Food Chem. 104: 1464 -1471

Wegulo SN, Zwingman MV, Breathnach JA, et al. (2011) Economic returns from fungicide application to control foliar fungal diseases in winter wheat. Crop. Prot. 30: 685-692


  • There are currently no refbacks.

Copyright (c)

                AR Journals

18K, Street 1st, Gaytri Vihar, Pinto Park, Gwalior, M.P. India

     (Design) 2009-2020


Follow @arjournals on Twitter