Screening of antioxidant, anti-acetylcholinesterase and antifungal activities and HPLC-MS identification of the bioactive phenolics of Eclipta alba (L.) Hassk.

Guenné Samson, Hilou Adama, Meda Nag Tiero Roland, Ouattara Nabèrè, Tiendrebéogo Martin, Millogo-Rasolodimby Jeanne, Nacoulma Odile Germaine

Abstract


Eclipta alba is commonly used in traditional medicine for several ethnobotanical properties. Researchers have isolated several bioactive compounds of this plant. In Burkina Faso, this medicinal plant is mainly used for its antifungal properties.

Our study aimed to evaluate the antioxidant, acetylcholinesterase inhibitory, antifungal activities and to quantify polyphenols from Eclipta alba extracts using HPLC-MS. The crude extract (CE) and fractions of this plant were used for these activities through standard methods. Ethyl acetate fraction (EAF) and acetonitrile fraction (ACNF) have been the potential inhibitors of the liver lipids peroxidation (According to the thiobarbituric acid method) with the percentages of 65.48% and 65.13%, respectively. Concerning the ABTS radical cation scavenging capacity descript by Guenné, ACNF (67.28 mg T/g of extract) and butanol fraction (BF) (66.03 mg T/g of extract) have presented the highest activities. BF has also presented an interesting acetylcholinesterase (AChE) inhibitory activity with a percentage of 55.82%. For antifungal activity, the crude extract and ACNF have given the better MIC values against C. albican and T. beigelii with a concentration of 1.25 mg/mL using microplate method descript by Eloff. The strain C. krusei was only sensitive to butanol fraction. The polyphenols identified and quantified using HPLC-MS are: gentisic acid, caffeic acid, chlorogenic acid, p-coumaric acid, apigenin, luteolin and quercetin.

The butanol fraction of E. alba presented the best activities for the tests performed; this fraction is a good ways to isolate new anti-fungal and anti-acetylcholinesterase compounds.


Keywords


E. alba; Antioxidant; anti-acetylcholinesterase; Anti-fungal; Polyphenols HPLC-MS

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References


. Nacoulma OG: Plantes médicinales et pratiques médicales traditionnelles au Burkina Faso: Cas du plateau central, Tome II. Thèse de doctorat d’état ès sciences naturelles, Université de Ouagadougou; Département de Biochimie-Microbiologie, 1996.

. Gautam R, Saklani A, Jachak SM. Indian medicinal plants as a source of antimycobacterial agents. Journal of Ethnopharm 2007; 110: 200-234.

. Panchal AH, Patel RK, Pundarikakshudu K, Bhandari A. Preliminary Phytochemical Screening and Evaluation of Anti-inflammatory Activities of Extract of Leaves of Eclipta alba L. (Asteraceae). Journal of Pharmacognosy and Herbal Formulations 2011; 1 (6): 28-36.

. Guenné S, Ouattara N, Hilou A, Millogo JF, Nacoulma OG. Antioxidant, enzyme inhibition activities and polyphenol contents of three Asteraceae species used in burkina faso traditionally medicine. Int J Pharm Pharm Sci 2011; 3 (5): 524-528.

. Syed DS, Deepak M, Yogisha S, Chandrashekar AP, Muddarachappa KA, D’Souza P, Agarwal A, Venkataraman BV. Trypsin Inhibitory Effect of Wedelolactone and Demethylwedelolactone. Phytother. Res. 2003; 17: 420-421.

. Pithayanukul P, Laovachirasuwan S, Bavovada R, Pakmanee N, Suttisri R. Anti-venon potential of butanolic extract of Eclipta prostrate against Malayan pit viper venon. Journal of Ethnopharm 2004; 90: 347-352.

. Tewtrakul S, Subhadhirasakul S, Cheenpracha S, Karalai C. HIV-1 Protease and HIV-1 Integrase Inhibitory Substances from Eclipta prostrata. Phytother. Res. 2007; 21: 1092-1095.

. Reddy SL, Fayazuddin Md, Reddy NCG, Ahmad A, Reddy GS, Raod PS, Reddy BJ, Fros RL. Characterisation of bhringaraj and guduchi herb by ICP MS analysis, optical absorption, infrared and EPR spectroscopic methods. Spectrochimica Acta Part A 2008; 71: 31-38.

. Lee MK, Ha NR, Yang H, Sung SH, Kim GH, Kim YC. Antiproliferative activity of triterpenoids from Eclipta prostrata on hepatic stellate cells. Phytomedicine 2008; 15: 775-780.

. Lee MK., Ha N.R., Yang H., Sung S.H., Kim YC. Stimulatory Constituents of Eclipta prostrata on Mouse Osteoblast Differentiation. Phytother. Res. 2009; 23: 129- 131.

. Ebrahimi A, Schluesener H. Natural polyphenols against neurodegenerative disorders: Potentials and pitfalls. Ageing Research Reviews 2012; 11: 329- 345.

. Su XYZ, Wang, Liu J. In vitro and in vivo antioxidant activity of Pinus koraiensis seed extract containing phenolic compounds. Food Chem.2009; 117 (4): 681- 686.

. Sombié PEAD, Hilou A, Coulibaly AY, Tibiri A, Kiendrebeogo M, Nacoulma OG. Brain protective and Erythrocytes Hemolysis Inhibition Potencials from Galls of Guiera senegalensis Gmel (Combretaceae). Journal of Pharmacology and Toxicology 2011; 6 (4): 361-370.

. Kiendrebeogo M, Coulibaly AY, Nebie RCH, Zeba B, Lamien CE, Lamien-Meda A, Nacoulma OG. Antiacetylcholinesterase and antioxidant activity of essential oils from six medicinal plants from Burkina Faso. Rev. Bras. Farmacogn. Braz. J. Pharmacogn 2011; 21 (1): 63-69.

. Bauer AW, Kirby WMM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966; 43: 493-496.

. Paulsamy S, Thambiraj J. Evaluation of antimicrobial efficacy of the folklore medicinal plant, Acacia caesia (L.) Wild. Asian J Pharm Clin Res 2011; 4 (3): 103-105.

. Eloff JN. A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta Medica 1998; 64: 711-713.

. Salem HF, Eid KAM, Sharaf MA. Formulation and evaluation of silver nanoparticles as antibacterial and antifungal agents with a minimal cytotoxic effect. International Journal of Drug Delivery 2011; 3 (2): 293-304.

. Cai Y, Luo Q, Sun M, Corke H. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci. 2004; 74 (17): 2157-2184.

. Xu Y, Zhang J, Xiong L, Zhang L, Sun D, Liu H. Green tea polyphenols inhibit cognitive impairment induced by chronic cerebral hypoperfusion via modulating oxidative stress. Journal of Nutritional Biochemistry 2010; 21: 741-748.

. Weng CJ, Chen MJ, Yeh CT, Yen GC. Hepatoprotection of quercetin against oxidative stress by induction of metallothionein expression through activating MAPK and PI3K pathways and enhancing Nrf2 DNA-binding activity. New Biotechnology 2011; 28 (6): 767- 777.

. Singh K, Singh N, Chandy A, Manigauha A. In vivo antioxidant and hepatoprotective activity of methanolic extracts of Daucus carota seeds in experimental animals. Asian Pacific Journal of Tropical Biomedicine 2012: 385-388.

. Tabassum N, Agrawal SS. Hepatoprotective activity of Eclipta alba hassk. against paracetamol induced hepatocellular damage in mice. JK - Practitioner 2004; 11 (4): 278-280.

. Kim DK, Lee SH., Choi JH, Lillehoj HS, Yu MH, Lee GS. The butanol fraction of Eclipta prostrata (Linn) effectively reduces serum lipid levels and improves antioxidant activities in CD rats. Nutr. Res 2008; 28: 550-554.

. Kim DK, Lee SH, Hong JH, Lillehoj HS, Park HJ, Rhie SG, Lee GS. The butanol fraction of Eclipta prostrata (Linn) increases the formation of brain acetylcholine and decreases oxidative stress in the brain and serum of cesarean-derived rats. Nutr. Res. 2010; 30 (8): 579–584.

. Abdel-Kader MS, Bahler BD, Malone S, Werkhoven MSM, Troon F, David, Wisse JH, Bursuker I, Neddermann KM, Mamber SW, Kingston DGI. DNA-Damaging Steroidal Alkaloids from Eclipta alba from the Suriname Rainforest. J. Nat. Prod. 1998; 61 (10): 1202- 1208.

. Zhang L, Cao H, Wen J, Xu M. Green tea polyphenol (−)-epigallocatechin- 3-gallate enhances the inhibitory effect of huperzine A on acetylcholinesterase by increasing the affinity with serum albumin. Nutr. Neurosci 2009; 12: 142-148.

. Guenné S, Hilou A, Ouattara N, Nacoulma OG. Antibacterial activity and Phytochemical composition of extracts of three medicinal Asteraceae species from Burkina Faso. Asian J Pharm Clin Res 2012; 5 (Suppl 2): 37- 44.

. Ryu HW, Curtis-Long MJ, Jung S, Jeong IY, Kim DS, Kang KY, Park KH. Anticholinesterase potential of flavonols from paper mulberry (Broussonetia papyrifera) and their kinetic studies. Food Chemistry 2012; 132: 1244-1250.


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