Total flavonoids, phenolics, tannins and antioxidant activity in seeds of lentil and grass pea

Dibyendu Talukdar


Lentil (Lens culinaris Medik.) and grass pea (Lathyrus sativus L.) are rich in polyphernols and antioxidants and in constant use as food and nutritional supplements in many traditional diets throughout the world. Both plants reportedly possess beneficial effects in jaundice, high fever, measles, diabetes, and cardiac problem.  Despite immense potentiality, limited work was done regarding antioxidant potential in different genotypes of these two legumes, useful in phytomedicinal and pharmacological formulations.

The objective of the present study was to assess the antioxidant composition and activity of ethanolic extract (ethanol: water, 80:20 v/v) of seeds (edible grain) of three improved genotypes each in lentil and grass pea. Total phnolics and flavonoids were estimated by the Folin–Ciocalteu colorimetric method and aluminium chloride method, respectively. Condensed tannin (pro anthocyanidins) content was determined using leucocyanidin (LE) equivalent. Antioxidant activity was ascertained by different in vitro protocols such as DPPH, β-carotene bleaching assay, reducing power and free radical scavenging activity.

Among the genotypes, IPL 406 in lentil and BioL 212 in grass pea showed the best performance with highest phenolic and flavonoid content and superior antioxidant activity as evidenced in potential FRAP, bleaching assay and by the low IC50 values in DPPH, hydroxyl and superoxide radical scavenging assay compared to WBL 58 and B 256 in lentil and B1 and BioR 231 in grass pea. The six genotypes differed significantly (P < 0.05) in antioxidant content and activity.

Both legumes are potential enough as phytomedicinal and functional foods with ample scope for genotype-wise phyto-nutrient profiling.


Lens culinaris; Lathyrus sativus; polyphenols; antioxidant; β-carotene bleaching assay; free radical scavenging

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. Xu B, Chang SKC. Phenolic substance characterization and chemical and cell-based antioxidant activities of 11 lentils grown in the Northern United States. J Agric Food Chem. 2010; 58: 1509-1517.

. Talukdar D, Talukdar T. Traditional food legumes in Sikkim Himalayas: Preparation of foods, uses and ethnomedicinal perspectives. International Journal of Current Research. 2012; 4: 64-73.

. Amarowicz R, Estrella I, Hernández T, Dueñas M, Agnieszka T, Kosińska A, Pegg RB. Antioxidant activity of a red lentil extract and its fractions. Int J Mol Sci. 2009; 10: 5513 -5527.

. Takeoka GR, Dao LT, Tamura H, Harden L. A. Delphinidin 3-O-(2-O-β-D-glucopyranosyl-R-L-arabinopyranoside): a novel anthocyanin identified in beluga black lentils. J Agric Food Chem. 2005; 53: 4932–4937.

. Bazzano L A, He J, Ogden LG. Legume consumption and risk of coronary heart disease in US men and women. NHANES I epidemiologic follow-up study. ACC Curr J Rev. 2002; 11: 31–32.

. Jenkins DJA, Kendall CWC, Marchie A, Jenkins A L, Augustin, LSA, Ludwig D S, Barnard ND, Anderson JW. Type 2 diabetes and the vegetarian diet. Am J Clin Nutr. 2003; 78: 610S–616S.

. Pastor-Cavada E, Juan R, Pastor JE, Manuel Alaiz M, Vioque J. Antioxidant activity of seed polyphenols in fifteen wild Lathyrus species from South Spain. Food Sci Technol. 2009; 42: 705-709.

. Lambein F, Kuo Y-H., Eguchi K.K., Ikegamic F. 3-N-oxalyl-L-2,3-diaminopropanoic acid, a multifunctional plant metabolite of toxic reputation. Arkivoc. 2007; ix: 45–52.

. Talukdar D. Dwarf mutations in grass pea (Lathyrus sativus L.): Origin, morphology, inheritance and linkage studies. J Genet. 2009; 88: 165-175.

. Kumar S, Bejiga G, Ahmed S, Nakkoul H, Sarker A. Genetic improvement of grass pea for low neurotoxin (b-ODAP) content. Food Chem Toxicol. 2011; 49: 589-600.

. Bhutia Z, Ghosh A, Sherpa ML, Pal R, Mohanta PK, Serum malodealdehyde level: Surrogate stress marker in the Sikkimese diabetics. Journal of Natural Science, Biology and Medicine. 2011; 2: 107-112.

. Gupta PC, Sharma N, Rao Ch V. Comparison of the antioxidant activity and total phenolic, flavonoid content of aerial part of Cleome viscosa L. International Journal of Phytomedicine. 2011; 3: 386-391.

. Agarwal PK, Katiyar AK. Validation of chickpea-STMS markers and DNA fingerprinting in lentil (Lens culinaris subsp. culinaris) cultivars of India. Indian J Genet. 2008; 68: 149-156.

. Talukdar D. Cytogenetic characterization of seven different primary tetrasomics in grass pea (Lathyrus sativus L.). Caryologia.2008; 61: 402-410.

. Talukdar D. Reciprocal translocations in grass pea (Lathyrus sativus L.). Pattern of transmission, detection of multiple interchanges and their independence. J Hered. 2010; 101: 169-176.

. Talukdar D. The aneuploid switch: Extra-chromosomal effect on antioxidant defense through trisomic shift in Lathyrus sativus L. Indian Journal of Fundamental and Applied Life Sciences. 2011;1: 263-273.

. Dixon RA, Sumner LW. Legume natural products: Understanding and manipulating complex pathways for human and animal health. Plant Physiol. 2003; 131: 878-885.

. Boateng J, Verghese M, Walker LT, Ogutu S. Effect of processing on antioxidant contents in selected dry beans (Phaseolus spp. L.). LWT-Food Sci Technol. 2008; 41: 1541-1547.

. Talukdar D. Flavonoid-deficient mutants in grass pea (Lathyrus sativus L.): Genetic control, linkage relationships, and mapping with aconitase and S nitrosoglutathione reductase isozyme loci. The Scientific World Journal. Volume 2012, Article ID 345983, 11 pages, doi:10.1100/2012/345983.

. Hooda MS, Pal R. Antioxidant potential and free radical scavenging activity by Cicer arietinum L. Int J Pharm Bio Sci. 2012; 3: 274-281.

. Rekha PS, Girija K, Ramadasan K. Antioxidant activity of Brahma rasayana. Indian Journal of Experimental Biology.2001; 39: 447–452.

. Benzie I F, Strain J J. Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymol. 1999; 299: 15-27.

. Kroyer GTh, Red clover extract as antioxidant active and functional food ingredient. Innovative Food Sci Emer Tech. 2004; 5: 101-105.

. SelvaKumar P, Kaniakumari D, Loganathan V. Phytochemical screening and antioxidant activity of red flowered Mirabilis jalapa leaf in different solvent. Int J Pharm Bio Sci. 2012; 3: 440-446.

. Zia-Ul-Haq M, Shahid SA, Ahmad S, Qayum M, Rasool N. Mineral contents and antioxidant potential of selected legumes of Pakistan. Journal of Medicinal Plants Research. 2012; 6: 4735-4740.

. Gharachorloo M,Tarzi BG, Baharinia M, Hemaci AH. Antioxidant activity and phenolic content of germinated lentil (Lens culinaris). Journal of Medicinal Plants Research. 2012; 6: 4562-4567.

. Tamburino R, Guida V, Pacifico S, Rocco M, Zarelli A, Parente A, Di Maro A. Nutritional values and radical scavenging capacities of grass pea (Lathyrus sativus L.) seeds in Valle Agricola district, Italy. Australian Journal of Crop Science. 2012; 6: 149-156.

. Talukdar D, Biswas AK. Induced seed coat colour mutations and their inheritance in grass pea (Lathyrus sativus L.). Indian J Genet.2005; 65: 135-136.

. Devasagayam TPA, Tilak JC, Boloor KK, Sane KS, Ghaskadbi SS, Lele RD. Free radicals and antioxidants in human Health: Current status and future prospects. Journal of The association of Physicians of India. 2004; 52: 795-804.

. Kumar JK, Devi Prasad AG, Richard SA. In vitro Antioxidant activity and preliminary phytochemical analysis of medicinal Legumes. Journal of Pharmacy Research. 2012; 5: 3059-3062.

. Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacognosy Rev. 2010; 4: 118-126.

. Talukdar D. Ascorbate deficient semi-dwarf asfL1 mutant of Lathyrus sativus exhibits alterations in antioxidant defense. Biologia Plantarum. 2012; 56: 675-682.

. Talukdar D. An induced glutathione-deficient mutant in grass pea (Lathyrus sativus L.): Modifications in plant morphology, alteration in antioxidant activities and increased sensitivity to cadmium. Bioremediation, Biodiversity and Bioavailability. 2012; 6: 75-86.

. Eberhardt M V, Kobira K, Keck A S, Juvik JA, Jeffery EH. Correlation analyses of phytochemical composition, chemical, and cellular measures of antioxidant activity of broccoli (Brassica oleracea L. var. italica). J Agric Food Chem. 2005; 53: 7421–7431.


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