Antioxidant activity and total phenolic content of various extracts from Mimosa hamata Willd., Mimosaceae

Renu Singh, Satish C. Jain, YT Jasrai

Abstract


Jinjani (Mimosa hamata Willd., Mimosaceae) is widely distributed in India and Pakistan, which contains several interesting bioactive constituents and possesses health promoting properties. In this study, the antioxidant activity with the total phenolic content of different successive extracts (pet. ether, chloroform, n-butanol and water) from leaves, stem, root and seeds of M. hamata were assessed in an effort to compare and validate the antioxidant potential of the particular part of the plant. The antioxidant activity was determined by 2, 2- Diphenyl-1-picrylhydrazyl (DPPH) assay and total phenolic content was estimated by using Folin-Ciocalteu’s reagent. All tested extracts possessed appreciable antioxidant potential but n-butanol extract of roots was significantly higher in total phenolic content (73.16±0.19 mg GAE/g extract) and in antioxidant assay (IC50 = 5µg/ml). It is also noteworthy that in n-butanol extract of roots higher yield (85.60%) was also observed. This study validates the antioxidant potential of the roots in M. hamata and the positive relationship between total phenolic content and antioxidant activity.


Keywords


Mimosa hamata Willd., total phenolic content, antioxidant activity, folin-Ciocalteu’s reagent, 2,2-diphenyl-1-picryhydrazyl (DPPH), ascorbic acid.

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References


. Hussain N, Modan MH, Shabbir SG, Zaidi SAH. Antimicrobial principles in Mimosa hamata. J. Nat. Prod. 1979;42:525-527.

. Patel NK. Ethnomedicinal plants used for impotence, frigidity and sexual weakness in Danta Taluka (Gujarat). Life Sci. Leaf. 2010;1:14-21.

. Nadkarni AK, Nadkarni KM. Indian Materia Medica. Popular Book Depot, Bombay 1954.

. Chopra RN, Nayar SL, Chopra IC. Glossary of Indian Medicinal Plants. CSIR, New Delhi 1956.

. Mehta BK, Sharma S, Dubey A. 4-Ethylgallic acid from two Mimosa species. Phytochem 1998;27:3004-3005.

. Jain R, Arora R. 3-O-L-Arabinosyl-D-glucosyl morolic acid from Mimosa hamata. J. Med. Arom. Plant Sci. 1997;19:22-23.

Jain R, Arora R, Jain SC. Saponins from the roots of Mimosa hamata Willd. Indian J. Chem. 1997b;36B:61-64.

. Mukadam DS, Balkhande LD, Umalkar GV. Antifungal activities in deproteinized leaf extract of weeds and nonweeds. Indian J. Microbiol. 1976;16:78-79.

. Umalkar GV, Mukadam DS, Mehemiah AKM. Sci. Cult. 1977;43:437.

. Jain R, Arora R, Alam S, Jain SC. Pharmacological evaluation of Mimosa hamata roots. Fitoterapia 1997a;68:377-378.

. Jain SC, Jain R, Vlietinck AJ. In vivo and in vitro antimicrobial efficacy of Mimosa hamata. Indian J. Biotech. 2004;3:271-273.

. Jain SC, Jain R, Singh R. Antioxidant activity of mimonosides isolated from in vitro regenerated plants of Mimosa hamata Willd. Indian J. Plant Physiol. 2009;14:124-129.

. Singh R, Jain SC, Jain R. Antioxidant activity of some medicinally important arid zone plants. Asian J. Exp. Sci. 2009;23(1):215-221.

. Scalbert A, Williamson G. Dietary intake and bioavailability of polyphenols. J. Nutr. 2000;130:2073S-2085S.

. Hertog MGL, Kromhout D, Aravanis C, et al. Flavonoid intake and long-term risk of coronary heart disease and cancer in Seven Countries Study. Arch. Intern. Med. 1995; 155:381-386.

. Keli SO, Hertog MGL, Feskens EJM, Kromhout D. Dietary flavonoids, antioxidant vitamins, and incidence of stroke: the Zutphen study. Arch. Intern. Med. 1996; 154: 637-642.

. Steinetz KA, Potter JD. Vegetables, fruit, and cancer prevention: a review. J. Am. Diet. Assoc. 1996;96:1027-2039.

. Andriantsitohaina R. Regulation of vascular tone by plant polyphenols: role of nitric oxide. Gen. Physiol. Biophys. 1999;18(1):3-5.

. Facino RM, Carini M, Aldini G, Berti F, Rossoni G, Bombardelli E, Morazzoni P. Diet enriched with procyanidines enhances antioxidant activity and reduces myocardial post-ischemic damage in rats. Life Sci. 1999;64:627-642.

. Leikert JF, Rathel TR, Wohlfart P, Cheynier V, Vollmar AM, Dirsch VD. Red wine polyphenols enhance endothelial nitric oxide synthase expression and subsequent nitric oxide release from endothelial cells. Circulation 2002;106:1614-1617.

. Rice-Evans CA, Miller NJ, Paganga G. Structure antioxidant activity relationship of flavonoids and phenolic acids. Free Radical Biol. Med. 1996;20:933-956.

. Davalos A, Bartolome B, Gomez-Cordoves C. Antioxidant properties of commercial grape juices and vinegars. Food Chem. 2005;93:325-330.

. Zhang S, Bi H, Liu C. Extraction of bioactive components from Rhodiola sachalinensis under ultrahigh hydrostatic pressure. Sep. Purif. Technol. 2007;57:275-280.

. Fogliano V, Verde V, Randazzo G, Retieni X. A method for measuring antioxidant activity and its application to monitoring the antioxidant capacity of wines. J. Agric. Food Chem. 1999;47:1035-1040.

. Lee SK, Mbwambo ZH, Chung HS, Luyengi L, Games EJC, Mehta RG, Kinghorn AD, Pezzuto M. Evaluation of the antioxidant potential of natural products. Comb. Chem. High T. Scr. 1998;1(1):35-46.

. Bray HG, Thorpe WV. Analysis of phenolic compounds of interest in metabolism. Meth. Biochem. Anal. 1954;1:27-52.

. Ghasemzadeh A, Jaafar HZE, Rahmat A. Antioxidant activities, total phenolics and flavonoids contents in two varieties of Malaysia young ginger (Zingiber officinale Roscoe). Molecules 2010;15:4324-4333.

. Yingming P, Ping L, Hengshan W, Min L. Antioxidant activities of several Chinese medicinal herbs. Food Chem. 2004;88:347-350.

. Louli V, Ragoussis N, Magoulas K. Recovery of phenolic antioxidants from wine industry by-products. Bioresour. Technol. 2004;92:201-208.

. Evans WC. Pharmacognosy. Harcourt Pub. Ltd., United Kingdom 2002.

. Keevil JG, Osman HE, Reed JD, Folts JD. Grapes juice but not orange juice or grapefruit juice, inhibits human platelet aggregation. J Nutr. 2000;130:53-56.

. Ribereau-gayon P. Plant Phenolics. Oliver and Boyd, Edinburgh 1972..


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