Relaxant effect of Jatropha gossypiifolia L. on uterine smooth muscle

Antonio Marcus de Andrade Paes, Adriana Leandro Camara, Sônia Maria de Farias Freire, Marilene Oliveira da Rocha Borges

Abstract


Jatropha gossypiifolia L. (Euphorbiaceae) is popularly known in Brazil as pião roxo and extensively used for hypotensive, spasmolytic and tocolytic purposes. In the present work, ethanolic extract and fractions from aerial parts of J. gossypiifolia L. were assayed for their effects on calcium-evoked uterine smooth muscle contraction.

Rat uterus strips were incubated with ethanolic extract (0.1, 0.5 and 1.0 mg/ml), chloroformic fraction (0.25; 0.5 mg/ml) or aqueous fraction (0.25; 0.5 mg/ml). Ethanolic extract promoted a rightward displacement of calcium cumulative curves, as well as, reduced the maximal contractions in 27.3% and 80.3% (0.5 and 1.0 mg/ml, respectively). Clhoroformic fraction inhibited the responsiveness of uterine muscle to calcium causing the maximal contractile response to be reduced in 27.4 % and 45.1 %, respectively. On the other hand, these parameters were only slightly reduced in the presence of aqueous fraction.

Taken together, our results suggest that ethanolic extract and fractions from J. gossypiifolia reduce calcium-evoked contractile response of the uterine smooth muscle, corroborating its ethnopharmacological application as tocolytic remedy.


Keywords


Jatropha gossypiifolia L., uterine smooth muscle, calcium influx and relaxation

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References


Corrêa, M.P., L. de Azeredo Penna, and I.B.d.D. Florestal, Dicionário das plantas úteis do Brasil e das exóticas cultivadas. 1984: Ministério da Agricultura, Instituto Brasileiro de Desenvolvimento Florestal.

Lorenzi, H. and F.J. de Abreu Matos, Plantas medicinais no Brasil: nativas e exóticas. 2002: Instituto Plantarum de Estudos da Flora.

Duke, J.A., Handbook of Medicinal Herbs. 2002: CRC Press.

Abreu, I.C., A.S. Marinho, A.M. Paes, S.M. Freire, R.S. Olea, M.O. Borges, and A.C. Borges, Hypotensive and vasorelaxant effects of ethanolic extract from Jatropha gossypiifolia L. in rats. Fitoterapia, 2003. 74(7-8): p. 650-7.

Silva, S.d.N., I.C. Abreu, S.M.d.F. Freire, M.d.S.d.S. Cartágenes, R.M. Ribeiro, A.S.d. Castro, A.C.R. Borges, and M.O.d.R. Borges, Antispasmodic effect of Jatropha gossypiifolia is mediated through dual blockade of muscarinic receptors and Ca2+ channels. Revista Brasileira de Farmacognosia, 2011. 21: p. 715-720.

Ojewole, J.A. and O.O. Odebiyi, Mechanism of the hypotensive effect of tetramethylpyrazine, an amide alkaloid from the stem of Jatropha podagrica. Planta Med, 1981. 41(3): p. 281-7.

Ojewole, J.A. and O.O. Odebiyi, Neuromuscular and cardiovascular actions of tetramethylpyrazine from the stem of Jatropha podagrica. Planta Med, 1980. 38(4): p. 332-8.

Duarte, D.F.P., A.E.G. Sant'Ana, and J.B. Calixto, Analysis of the vasorelaxant action of jatrophone in the isolated aorta of the rat: influence of potassium channel blockers. European Journal of Pharmacology, 1992. 215(1): p. 75-81.

Calixto, J.B. and A.E.G. Sant'ana, Evidence for the mechanism of the inhibitory action of jatrophone in the isolated rat uterine muscle. General Pharmacology: The Vascular System, 1990. 21(1): p. 117-122.

Falodun, A., Z.M. Nworgu, and E. Osayemwenre, Smooth muscle relaxant evaluation of Jatropha Curcas Linn (Euphorbiaceae) and isolation of triterpenes. Niger J Physiol Sci, 2011. 26(2): p. 133-7.

Zhang, X.P., M.L. Zhang, X.H. Su, C.H. Huo, Y.C. Gu, and Q.W. Shi, Chemical constituents of the plants from genus Jatropha. Chem Biodivers, 2009. 6(12): p. 2166-83.

Silva, A.M., R.L. Brum, and J.B. Calixto, The relaxant action of jatrophone in rat portal vein. A comparison with protein kinase C inhibitors. Life Sci, 1995. 57(9): p. 863-71.

Falodun, A., U. Kragl, S.M. Touem, A. Villinger, T. Fahrenwaldt, and P. Langer, A novel anticancer diterpenoid from Jatropha gossypifolia. Nat Prod Commun, 2012. 7(2): p. 151-2.

Theoduloz, C., J.A. Rodriguez, M. Pertino, and G. Schmeda-Hirschmann, Antiproliferative activity of the diterpenes jatrophone and jatropholone and their derivatives. Planta Med, 2009. 75(14): p. 1520-2.

Calixto, J.B. and S. Loch, Ketamine-inhibition of calcium-induced contractions in depolarized rat uterus: a comparison with other calcium antagonists. Br J Pharmacol, 1985. 85(1): p. 189-95.


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