Ethnomedicinal, Antibacterial and Antifungal Potentiality of Centella asiatica, Nerium indicum and Cuscuta reflexa - Widely Used In Tiwa Tribe of Morigaon district of Assam, India.

Debajit kalita, Jagat Saikia


In Assam, Approximately 17 different types of Tribes are residing from immemorial times. They have lots of knowledge to remedy disease by using various medicinal plants. The aim of our study is to extract out the knowledge of those plants having the medicinal values and examine the laboratory based antibacterial and antifungal property. The selection of these three plants was based on highly uses by the Tiwa Tribes in gastrointestinal troubles and skin diseases. Aquous and 50% ethanolic extract was prepared from the plants and screened against enteropathogenic bacteria E.coli (MTCC723), Bacillus subtilis (MTCC10619) and Staphyllococcus aureus (MTCC96). Antifungal activity were tested against Aspergillus niger and Candida albicans. Centella asiatica and Nerium indicum showed good results against E.coli and Bacillus subtilis and Cuscuta reflexa showed higher activity against S. aureus. In case of antifungal activity, Centella asiatica results were found fruitful in comparison to the others.


Antibacterial; Antifungal; E.coli; Staphyllococcus aureus; Aspergillus niger; MTCC

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Rang, H.P., Dale, M.M., Ritter, J.M., Moore, P.K., 2003. Pharmacology, 5th ed. Churchill, Livingstones. Edinburgh, p. 797.

Clardy, J., Fischbach, M.A., Walsh, C.T., 2006. New antibiotics from bacterial natural products. Nature Biotechnology 24, 1541–1550.

Cowan, M.M., 1999. Plant products as antimicrobial agents. Clinical Microbiology Reviews 12, 564–582.

Zubaır, Md., Rızwan, K., Rasool, N., Afshan, N., Shahid, M., Ahmed, V.U. 2011. Antimicrobial potential of various extract and fractions of leaves of Solanum nigrum. International Journal of Phytomedicine 3: 63-67.

Kalyani L., Lakshmana Rao, A., Mishra, U.S. 2011. Antibacterial and analgesic activity of leaves of Lantana camara. International Journal of Phytomedicine 3: 381-385

Mothana, R.A., Lindequist, U., 2005. Antimicrobial activity of some medicinal plants of the island Soqotra. J.Ehnopharmacol 96,177-81.

Tally, F.P. and DeBruin, M.F., 2000. Journal of Antimicrobial Chemotherapy 46, 523-526.

Newman, D.J., Cragg, G.M., 2007. Natural products as source of new drugs over the last 25 years. Journal of Natural products 70, 461-477.

Gibbons, S., 2005. Plants as a sources of bacterial resistance modulators and antiinfective agents. Phytochemistry Reviews 4, 63-78.

Pauli, G.F., Case, R.J., Inui, T., Wang. Y., Cho, S., Fischer, N.H., Franzblau, S. G., 2005. New perspectives on natural products in TB drug research. Life Sciences 78, 485- 494.

Valgas, C., de Souza, S.M., Smânia, E.F.A., Smânia Jr., A., 2007. Screening methods to determine antibacterial activity of natural products. Braz. J. Microbiol. 38, 369–380.

Tripoli, E., La Guardia, M., Giammanco, S., Di Majo, D., Giammanco, M., 2007. Citrus flavonoids: molecular structure, biological activity and nutritional properties: a review. Food Chem. 104, 466–479.

Conner, D. E. (1993). Naturally occurring compounds. In P. M. Davidson, & A. L. Branen (Eds.), Antimicrobials in foods (pp. 441–468). New York: Marcel Dekker.

Robert AP, Lorraine F, Walter M, Ronald MR (2009). Laboratory exercises in Microbiology (3rd ed.). U.S: John Wiley & Sons,Inc.

Ahmed N, Uzir B, Ayaz S, Ahmed VU(2008). Antibacterial activity of marine bacteria from Arabian Sea of Pakistan. J. Microbiol., 4(2): 1 – 11.

Wang, H., Cheng, H. Wang, F., Wei, D. and Wang, X., 2010. An improved 3-(4,5- dimethylthiazol 2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction assay for evaluating the viability of Escherichia coli cells. J. of Microbiol. Methods, 82 (2010) 330 –333.

Burt, S., 2004. Essential oils: their antibacterial properties and potential applications in foods—a review. Int. J. Food Microbiol. 94, 223–253.

ESCMID, 2000. Determination of minimum inhibitory concentrations (MICs) of antibacterial agents by agar dilution. European Committee for Antimicrobial Susceptibility Testing (EUCAST) of the European Society of Clinical Microbiology and Infectious Diseases (ESCMID). EUCAST DEFINITIVE DOCUMENT E. Def Copyright by the European Society of Clinical Microbiology and Infectious Diseases.June 2000.

Natarajan, P., Katta, S., Andrei, I., Babu Rao Ambati, V., Leonida, M., Hass, G.J., 2008. Positive antibacterial co-action between hop (Humulus lupulus) constituents and selected antibiotics. Phytomedicine 15, 194–201.

Sudjana, A.N., D'Orazio, C., Ryan, V., Rasool, N., Ng, J., Islam, N., Riley, T.V., Hammer, K.A.,2009. Antimicrobial activity of commercial Olea europaea (olive) leaf extract. Int. J. Antimicrob. Agents 33, 461–463.

Stratford, M., Eklund, T., 2003. Organic acids and esters. In: Russell, N.J., Gould, G.W. (Eds.), Food Preservatives. Kluwer Academic/Plenum Publishers, London, pp. 48–84.

Davidson, P.M., 2001. Chemical preservatives and natural antimicrobial compounds, In: Doyle, M.P., Beuchat, L.R., Montville, T.J. (Eds.), Food Microbiology: Fundamental and Frontiers, 2nd ed. ASM Press, Washington DC, pp. 593–627.

Ikigai, H., Nakae, T., Hara, Y., Shimamura, T., 1993. Bactericidal catechins damage the lipid bilayer. Biochemistry Biophysics Acta 1147, 132–136.

Otake, S., Makimura, M., Kuroki, T., Nishihara, Y., Hirasawa, M., 1991. Anticaries effects of polyphenolic compounds from Japanese green tea. Caries Research 25, 438–443.

Akagawa, M., Shigemitsu, T., Suyama, K., 2003. Production of hydrogen peroxide by polyphenols and polyphenol-rich beverages under quasi-physiological conditions. Bioscience, Biotechnology, and Biochemistry 67, 2632–2640.

Ceylan, E., Kang, D.E., Fung, D.Y.C., 1998. Reduction of E. coli O 157:H7 in ground meat by selected spices. Annual Meeting of Institute of Food Technologist, Atlanta, June 20–24.

Sallam, K.I., Ishioroshi, M., Samejima, K., 2004. Antioxidant and antimicrobial effects of garlic in chicken sausage. LWT—Food Science and Technology 37, 849–855.

Gulluce, M., Sahin, F., Sokmen, M., Ozer, H., Daferera, D., Sokmen, A., Polissiou, M., Adiguzel, A., Ozkan, H., 2007. Antimicrobial and antioxidant properties of the essential oils and methanol extract from Mentha longifolia L. ssp. Longifolia. Food Chemistry 103, 1449–1456.

Tassou, C., Drosinos, E.H., Nychas, G.J.E., 1995. Effects of essential oils from mint (Mentha piperita) on Salmonella Enteritidis and Listeria monocytogenes in model food systems at 4 °C and 10 °C. Journal of Applied Bacteriology 78, 593–600.

Nguyen, P., Mittal, G.S., 2007. Inactivation of naturally occurring microorganisms in tomato juice using pulsed electric field (PEF) with and without antimicrobials. Chemical Engineering and Processing 46, 360–365.

Singh, C., Singh, K.S., Nath, G. and Rai, N.P. 2011. Anti-mycobacterial activity of Piper longum L. fruit extracts against multi drug resistant Mycobacterium Spp. International Journal of Phytomedicine 3: 353-361


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