Pharmacological and Toxicological effects of Aqueous Acetone Extract of Sida alba L. (Malvaceae) in animals model

Konaté kiessoun, M. Ouédraogo, J. F. Mavoungou, A.N. Lepengué, A. Souza, B.M’ Batchi, O.G. Nacoulma

Abstract


The present study was conducted to evaluate the pharmacological and toxic effects of aqueous acetone extract of Sida alba L. a Malvaceae species, in mice Swiss and albinos Wistar rats. In acute toxicity test, mice received doses of this extract by intraperitoneal route with LD50 value of 3200 mg/kg. In sub-acute toxicity test, albinos Wistar rats were treat by gavage during 28 days with different doses of aqueous acetone extracts of Sida alba L., (75, 100 and 150 mg/kg). About to the pharmacological properties, the results varied widely in dose of extract and weight of rats and did not show clinical correlations. We undertook this study of extracts in order to provide a scientific basis for the traditional use of Sida alba L., in traditional medicine particularly to treat hepatitis B. Our results of this study appeared to show the safety of acute and sub-acute toxicities of extract from Sida alba L., which can therefore be continuously used with safety in traditional medicine. Statistical studies revealed that there is a low significant difference in body and organ weights, and biological parameters between control group and the treated assay groups (p<0.01 or p<0.05).


Keywords


Sida alba, LD50, toxicity, Biological parameters, mice and Wistar rats

Full Text:

PDF

References


Nacoulma OG. Medicinal plants and their traditional uses in Burkina Faso. Ph.D.Thesis, University of Ouagadougou. 1996; 328.

Konaté K, Souza A. Polyphenol Contents, Antioxidant and Anti-Inflammatory Activities of Six Malvaceae Species Traditionally used to treat Hepatitis B in Burkina Faso.

European Journal of Scientific Research. 2010; 44 (4), pp.570-580.

Dicko MS and LK Sikena. Legume Trees and Other Fodder Trees as Protein Source for Livestock. Proc. Of FAO Expert consult. Speedy A. and P. Pugciese (Eds.), www. FAO.ORG/DOCREP 1991.

Miller LC, Tainter ML. Estimation of the ED50 and its error by means of logarithmic probit graph paper. In: Proceedings of the society for Experimental Biology and Medicine. 1944; 57: 261-264.

Trinder P. Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Ann. Clin. Biochem.1969; 6, 24.

Burrin JM, Price CP., 1985. Measurement of blood glucose. Ann. Clin. Biochem. 1985; 22, 327.

Schumann G. Clin Chem Lab Med. 2002a; 40, 718.

Schumann G. Clin Chem Lab Med. 2002b; 40, 752.

German Society for Clinical Chemistry. Z.Klin. Chem. 1972; 10, 281.

Committee on Enzymes of the Scandinavian Society for Clinical Chemistry and Physiology. Scand. J. Clin. Lab. Invest. 1974 ; 33, 291.

Fossati P, Prencipe L, Berti G. Clin. Chem. 1983; 29, 1494.

Fossati P. Use of 3,5-dichloro-2-hydroxy-benzenesulfonic acid/4-aminophenazone chromogenic system in direct enzymatic assay of uric acid in serum and urine. Clin. Chem. 1980; 26, 227.

Fawcett JK, Scott JE. A Rapid and Precise Method for determination of urea. J.Clin.Path. 1960; 13, 156.

Fossati P, Prencipe L. Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. Clin. Chem. 1982; 28, 2077

Allain CC. Clin. Chem. 1974; 20, 470.

Sherwin JE, Thompson C. Liver function. Clinical Chemistry: Theory, Analysis, Correlation, 4th Ed., Kaplan, L.A., Pesce, A.J., Kazmierczak, S.C. (Mosby Inc. EDS St Louis USA), 493 and appendix. 2003.

Diez J. (1989). Toxicology: Basic principles and chemical impact. In: Pharmacology: Fundamental principles to therapeutic applications. Ed. SLATKINE. GENEVE. 1989; 33-44.

Clarke EGC, Clarke. Veterinary Toxicology. Cassel and Collier Macmillan Publishers. 1977; 268-277.

Raza M, AI-Shabanah OA, El-Hadiyah TM, Al-Majed AA. 2002. Effect of prolonged vigabatrin on hematological and biochemical parameters in plasma, liver and kidney of Swiss albino mice. Scientia Pharmaceutica. 2002; 70:135-145.

Teo S, Stirling D, Thomas S, Hoberman A, Kiorpes A, Khetani V. A 90-day oral gavage toxicity study of D-methylphenidate and D L-methylphenidate in Sprague-Dawley rats. Toxicology. 2002; 179:183-196.

Fahey Jr PR and HG Jung. Phenolic compound in forage and Fibrous Feedstuffs. In: Toxicants of plant origin. IV phenolics. Cheeke. P.R.(Eds.) CRC Press, Florida. 1989; 123-190.

Lewis RW, Billington R, Debryune E, Gamer A, Lang B, Carpanini F. Recognition of adverse and nonadverse effects in toxicity studies. Toxicologic Pathology. 2002; 30: 66-74.

Corns CM. Herbal remedies and clinical biochemistry. Annals of Clinical Biochemistry. 2003; 40: 489-507.

Hilaly J, El Israili, ZH, Lyoussi B. Acute and chronic toxicological studies of Ajuga iva in experimental animals. Journal of Ethnopharmacology. 2004; 91: 43-50.

Isnard BC, Deray G, Baumelou A, Le Quintree M, Vanherweghem JL. Herbs and the kidney. American Journal of Kidney Diseases. 2004; 44, 1-11.

Saad B, Azaizeh H, Abu-Hijleh G, Said S. Safety of traditional Arab herbal medicine. Evidence-based Complementary and Alternative Medicine. 2006; 3: 433-439.

Newman DJ, Price CP. Renal function and nitrogen metabolites. In: Burtis, C.A., Ashwood, E.R. (Eds.), Tietz textbook of Clinical Chemistry. W.B. Saunders Compagny, Philadelphia 1999; 1204-1270.

Tolman KG R. Liver function. In: Burtis, C.A., Ashwood, E.R. (Eds.), Tietz textbook of Clinical Chemistry. W.B. Saunders Compagny, Philadelphia. 1999; 1125-1177.

Meda NTR, A Lamien-Meda, M Kiendrebeogo, CE Lamien and AY Coulibaly. In vitro Antioxidant, xanthine oxidase and acetylcholinesterase inhibitory activities of Balanites aegyptiaca (L.) Del. (Balanitaceae). Pak. J. Biol. 2010; 13:362-368.

Sigma Diagnostics. Transaminases (ALP/GPT) and AST/GOT Quantitative Colorimeter Determination in serum, plasma or Cerebrospinal Fluid Procedure. 1985; 505.

Tilkian S M. Clinical Implicationss of Laboratory Tests. The C.V.Moshy Compagny, Nissouri. 1979; 11-17.

Woodman D D. Study of serum toxicity. J.Applied Toxicol. 1988; 84: 249-254.

Boyd JW. Serum enzymes in the diagnosis of diseases in man and animals. J.Comp.Pathol. 1988; 9:382-403.

Bush BM. Intrepretation of Laboratory Results for Small Animal Clinicians. Backwell Scientific Publication, London.1991.

Dial JM. Clinicopathological evaluation of the liver. Vet.Clin. 1995; 25: 275-293.

Odutola AA. Rapid interpretation of Routine Clinical Laboratory Test. Asekome, S.and Co. Zaria. 1992; 1-30.

Rabo JS. Toxicity studies and Trypanosuppressive effects of sterm bark extract of Butyrospermum paradoxum in laboratory animals. Ph.D Thesis, University of Maiduguri, Maiduguri.1988.


Refbacks

  • There are currently no refbacks.
';



Copyright (c)

                AR Journals

18K, Street 1st, Gaytri Vihar, Pinto Park, Gwalior, M.P. India

              Copyright@arjournals.org (Design) 2009-2021

 

Follow @arjournals on Twitter