Acute toxicity, anti-inflammatory and antinociceptive investigations of extracts from Ampelocissus africana (Lour) Merr (Vitaceae) rhizomes

Wendkouni Leila Marie Esther Belem-Kabré, Noufou Ouédraogo, Adjaratou Compaoré-Coulibaly, Mariam Nebié-Traoré, Tata Kadiatou Traoré, Sylvain Ilboudo, Constantin Boladé Atchadé, Abdoul Gilchrist Laurent Boly, Marius Lompo, Martin Kiendrebeogo, Sylvin Ouédraogo



Ampelocissus africana (Lour) Merr (Vitaceae) is a plant used in traditional medicine in Burkina Faso in the treatment of wounds, edema, drops, infections. This study focused on pharmacological investigations effects of methanolic and aqueous extracts from Ampelocissus africana (Lour) Merr rhizomes.

The acute toxicity of the extracts from Ampelocissus africana was performed under OECD 423 guidelines. The anti-inflammatory activity was evaluated in vivo using carrageenan-induced paw edema of mice at the doses to 50, 100, 200, 400, 600 mg/kg body weight (bw).  The antinociceptive activity of the extracts was determined using acetic acid, formalin nociception models in mice at the doses to 50, 100, 200, 400 and 600 mg/kg bw. The involvement of ATP-sensitive K+ channel pathway and opioid system were tested using glibenclamide, and naloxone respectively at the unique dose (400 mg/kg).

No mortality of mice were observed at dose of 2000 mg/kg b.w. The lethal dose (LD50) value estimated to 5000 mg/kg b.w. The tests were showed that the extracts exerted significant dose-dependent anti-inflammatory responses in the paw induced by carrageenan (from 37 to 72.90 percent inhibition), antinociceptives in acetic-induced abdominal contractions (from 25.10 to 63.08%), and in formaldehyde-induced paw licking (from 9.27 to 71.77%) tests. The pretreatment with naloxone and glibenclamide did not affect the antinociceptives effects.

The results suggested that the extracts from the rhizomes of Ampelocissus africana possessed anti-inflammatory and antinociception effects in mice. These effects could justify the uses of this plant in traditional medicine.



Ampelocissus africana; acute toxicity; inflammation; analgesic


[1]. Sosa S, Balic MJ, Arvigo R, Esposito RG, Pizza C, Altinier G, Tubaro A. Screening of the topical anti-inflammatory activity of some Central American plants. J Ethnopharmacol. 2002; 81: 211–215

[2]. Koech SC, Ouko RO, Michael NM, Ireri MM, Ngugi MP, Njagi NM. Analgesic Activity of Dichloromethanolic Root Extract of Clutia abyssinica in Swiss Albino Mice. Nat Prod Chem Res. 2017; 5:2

[3]. Wong RSY. Role of Nonsteroidal Anti-Inflammatory Drugs (NSAIDs) in Cancer Prevention and Cancer Promotion. Advances in Pharmacological Sciences. 2019; 2019 : 1–107.

[4]. Jordan S, White J. Non-steroidal anti-inflammatory drugs: the clinical issues. Nursing Standard. 2001; 15(23): 45-52

[5]. Achinto S, Muniruddin A. The analgesic and anti-inflammatory activities of the extract of Albizia lebbeck in animal model. Pak. J. Pharm. Sci. 2009; 22 (1): 74-77

[6]. Nacoulma OG. Plantes médicinales et pratiques médicinales traditionnelles au Burkina Faso - Cas du plateau central. Thèse de Doctorat d'Etat: Tome II, Université de Ouagadougou; 1996

[7]. Zimmermann M. Ethical guidelines for investigations of experimental pain in conscious animals. Pain. 1983; 16(2) :109-110

[8]. OCDE. Test No. 423. Acute Oral toxicity - Acute Toxic Class Method, OECD Guidelines for the Testing of Chemicals. OCDE. 2001; 4 :1-14

[9]. Winter CA, Risley EA, Nuss GW. Carrageenin-induced edema in hind paw of the rat as an assay for anti-inflammatory drugs. Proceedings of the Society for Experimental Biology and Medicine. 1962; 111: 544-547

[10]. Ouédraogo N, Tibiri A, Sawadogo RW, Lompo M, Hay AE, Koudou J, Dijoux MG, Guissou IP. Antioxidant anti-inflammatory and analgesic activities of aqueous extract from stem bark of Pterocarpus erinaceus Poir. (Fabaceae). Journal Med Plants Res. 2012; 5(10): 2047–53.

[11]. Sawadogo WR, Boly R, Lompo M, Some N, Lamien CE, Guissou IP, Nacoulma OG. Anti-inflammatory, analgesic and antipyretic activities of Dicliptera verticillata. Int J Pharmacol. 2006; 2 (4): 435–8.

[12]. Alves DP, Duarte IDG. Involvement of ATP sensitive K+ channels in the peripheral antinociceptive effect induced by dipyrone. European Journal of Pharmacology. 2002; 444(1-2): 47–52

[13]. Alam B, Akter F, Parvin N, Pia RS, Akter S, Chowdhury J, Sifath-E-Jahan K, Haque E. Antioxidant, analgesic and anti-inflammatory activities of the methanolic extract of Piper betle leaves. Avicenna Journal of Phytomedicine. 2013; 3(2): 112-125.

[14]. Suloon J, Shah AM, Ming-Tatt L, Akhtar MN, Perimal EK, Akira A, Israf DA, Sulaiman MR. Hexanic fraction of turmeric powder attenuates murine model of inducednociception and its possible mechanisms of action. Afr J Tradit Complement Altern Med. 2015; 12(6): 90-99

[15]. ONU. Globally Harmonized System of Classification and Labelling of Chemicals (GHS): Seventh revised edition, ONU, New York. 2017; 533pp

[16]. Di Rosa M, Girround JP, Willoughby DA. Studies on the acute inflammatory response induced in rats in different sites by carrageenan and turpertine. J. Pathology. 1971; 104: 15-29.

[17]. Hassan HS, Ahmadu AA, Hassan AS. Analgesic and anti-inflammatory activities of Asparagus africanus root extract. J. Trad. CAM. 2008 ; 5 (1) : 00 – 00

[18]. Sawadogo WR, Lompo M, Somé N, Guissou IP, Nacoulma OG. Anti-inflammatory, analgesic and antipyretic effects of Lepidagathis anobrya nees (acanthaceae). Afr J Tradit Complement Altern Med. 2011; 8(4): 420‐424

[19]. Gené RM, Segura L, Adzet T, Marin E, Iglesias J. Heterotheca inuloides: anti-inflammatory and analgesic effects. J Ethnopharmacol. 1998; 60: 157–162

[20]. Olorukoobaa AB, Odoma S. Elucidation of the possible mechanism of analgesic action of methanol stem bark extract of Uapaca togoensis pax in mice. J Ethnopharmacol. 2019; 245: 112156

[21]. Perimal E, Akhtar M, Mohamad A, Hanief KM, Hui MO, Syamimi K, Sulaiman MR. Zerumbone-Induced Antinociception: Involvement of the L-Arginine-Nitric Oxide-cGMP - PKC-K+ATP Channel Pathways. Basic Clin Pharmacol Toxicol. 2011; 108: 155–162.

[22]. Zainul AZ, Mohammad HAR, Rushduddin AJR, Mohd HMS, Maizatul HO, Siti FM, Tohid FO, Siew MC, Arifah AK. Antinociceptive Activity of Methanolic Extract of Clinacanthus nutans Leaves: Possible Mechanisms of Action Involved. Pain Research and Management. 2018; 2018: 9536406

[23]. Melo FHC, Rios ERV, Rocha NFM, Citó MCO, Fernandes ML, Pergentino de Sousa D, Mendes de Vasconcelos SM and Florenço de Sousa FC. Antinociceptive activity of carvacrol (5-isopropyl-2-methylphenol) in mice. Journal of Pharmacy and Pharmacology. 2012; 64: 1722–1729

[24]. Shibata M, Ohkubo T, Takahashi H, and Inoki R. Modified formalin test: characteristic biphasic pain response. Pain. 1989; 38: 347-352

[25]. Trescot A M. Opioid Pharmacology and Pharmacokinetics. Controlled Substance Management in Chronic Pain. 2016; 45–62

[26]. Mohammadifard F, Alimohammadi S. Chemical Composition and Role of Opioidergic System in Antinociceptive Effect of Ziziphora Clinopodioides Essential Oil. BCN. 2018; 9 (5): 357-366

[27]. Chiabrando C, Castelli MG, Cozzi E, Fanelli R, Campoleoni A, Balotta C, Latini R, Garattini S. Antiinflammatory action of salicylates: aspirin is not a prodrug for salicylate against rat carrageenin pleurisy. Eur J Phycol. 1989; 159: 257-264


  • There are currently no refbacks.

Copyright (c) 2020 Wendkouni Leila Marie Esther Belem-Kabré;Noufou Ouédraogo; Adjaratou Compaoré-Coulibaly; Mariam Nebié-Traoré; Tata Kadiatou Traoré; Sylvain Ilboudo; Constantin Boladé Atchadé; Abdoul Gilchrist Laurent Boly;, Marius Lompo; Martin Kiend

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

                AR Journals

18K, Street 1st, Gaytri Vihar, Pinto Park, Gwalior, M.P. India

     (Design) 2009-2021


Follow @arjournals on Twitter