Preliminary phytochemical screening and Evaluation of hepatoprotective activity of ethanolic extract of whole plant of Evolvulus alsinoides using CCl4 induced model in experimental animals

Gomathi Periasamy, Syeda Hafsa Mehjabeen, Krishna Prasad, Syeda Husna Fatima, Shalini Thakkallapelly

Abstract


Present study was conducted to evaluate the preliminary phytochemical screening and hepatoprotective activity of whole plant of Evolvulus alsinoides. The whole plant was defatted with petroleum ether and then extracted with 90 % ethanol. The phytochemical screening was done for ethanol extract using standard procedures. Acute toxicity was done using OECD 423 guidelines and the extract was found to be practically non-toxic upto a dose of 1500mg/kg b.w. when given orally. Hepatoprotective activity was evaluated using Carbon tetra chloride induced model in rats. In CCl4 induced mothod of 90% Ethanolic extract of whole plant of Evolvulus alsinoides (75 & 150mg/kg b.w.) produced significant dose dependent hepatoprotective activity. Histopathological studies could be carried out to assess the degree of damage. The hepatoprotective effect of the aforesaid extract was substantiated by pentobarbital sleeping time experiment in mice. The effect of the extracts at 150 mg/kg was compared to that of the reference drug, Silymarin (50 mg/kg). In vitro antioxidant studies were conducted to confirm the antioxidant mechanism involved in their hepatoprotective activity in CCl4-induced in rats. The presence of flavonoids could be responsible for hepatoprotective activity.


Keywords


Evolvulus alsinoides, phytochemmical screening, hepatoprotective, flavonoids, antioxidnant

References


. Akram Eidi, Pejman Mortazavi. Hepatoprotective activity of cinnamon ethanolic extract against CCl4 induced liver injury in rats, EXCLI Journal 2012;11:495-507

. T.S. Mohamed Saleem, C. Madhusudhana Chetty, S. Ramkanth, V.S.T. Rajan, K. Mahesh Kumar, Gauthaman. Hepatoprotective Herbs – A Review, Int. J. Res. Pharm. Sci. 2010;1(1) :1-5.

. Wink M. Introduction Biochemistry, role & biotechnology of secondary products Metabolism, CRC Press, Boca Ratom, FL, 1999; 1-16.

. Harsh Mohan. Text Book of Pathology. 6th ed. New Delhi: Jaypee Brothers; 2010.

. Barry Halliwell, John Gutteridge MC. Free radicals in biology and medicine. 3rd ed.NewYork: 2001, Oxford University press.

. Sethiya NK, Nahata A, Mishra SH, Dixit VK. An update on Shankhpushpi, a cognition-boosting Ayurvedic medicine. Zhong Xi Yi Jie He Xue Bao 2009; 7(11):1001-22

. Austin DF. Evolvulus alsinoides (Convolvulaceae): an American herb in the Old World. J Ethnopharmacol. 2008; 117(2):185-98.

. Gomathi D, Kalaiselvi M, Ravikumar G, Sophia D, Gopalakrishnan VK, Uma C. Secondary metabolite credentials of Evolvulus alsinoides by high performance thin layer chromatography (HPTLC). J Biomed Res. 2012; 26(4):295-302.

. Siripurapu KB, Gupta P, Bhatia G, Maurya R, Nath C, Palit G. Adaptogenic and anti-amnesic properties of Evolvulus alsinoides in rodents. Pharmacol Biochem Behav. 2005; 81(3):424-32.

. Shah JS, Goyal RK. Usage trends for memory and vitality-enhancing medicines: A pharmacoepidemiological study involving pharmacists of the Gujarat region. Int J Ayurveda Res. 2010; 1(3): 138–143.

. Schenkel EP, Gosmann G, Athayde ML. Saponinos, Farmacognosia: da planta ao medicamento, 6th ed, Porto Alegre: UFRGS, 2007; 711–740.

. Santos SC, de Mello JC. Taninos, Farmacognosia: da planta ao medicamento, 6th ed, Porto Alegre: UFRGS, 2007; 615–656.

. Farnsworth NR. Biological and phytochemical screening of plants. J Pharm Sci, 1996; 55, 225–276.

. Evans WC. 16th ed, London: Sauders Elsevier. Trease and evans’ pharmacognosy, 2009; 25-39.

. Harborne JB. A guide to modern techniques of plant analysis, 3rd ed, London, New York: Chapman and Hall, 1998.

. Pearson D. The chemical analysis of foods, Edinburgh, 7th ed, New York, 1976.

. OECD, Acute oral toxicity, Acute oral toxic class method guideline 423 adopted 17.12.2001, In Eleventh Addendum to the OECD guidelines for the testing of chemicals, Organisation for Economical Cooperation and Development, Paris, June, 2000.

. Manoj B, Aqueed K. Protective effect of Lawsonia alba Lam., against CCl4-induced hepatic damage in albino rats. Ind J Expt Biol 2003; 41: 85-87.

. Bergmeyer HU, Scheibe P, Wahlefeld AW. Optimization of methods for aspartate aminotransferase and alanine aminotransferase. Clin Chem 1978; 24: 58-61.

. King J. The hydrolases-acid and alkaline phospatase, Practical Clinical Enzymology, Van, D (ed.), Nostrand company Ltd, London, 1965; 191-208.

. Malloy HJ, Evelyn KA. The determination of bilirubin with the photoelectric colorimeter. J Biol Chem 1937; 119: 481-482.

. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin- phenol reagent. J Biol Chem 1951; 193: 265-275.

. Fujimoto JM, Pearce KB, Plaa JL. Barbiturate metabolism as affected by certain agents acting on liver. J. Pharmacol. Exp. Thera. 1960; 129: 139-143.

. Naskar S, Islam A, Mazumder UK, Saha P, Haldar PK, Gupta M. In Vitro and In Vivo Antioxidant Potential of Hydromethanolic Extract of Phoenix dactylifera Fruits. J. Sci. Res. 2010; 2 (1): 144-157.

. Dinis TCP, Madeira VMC, Almeida MLM. Action of phenolic derivates (acetoaminophen, salycilate and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch. Biochem. Biophys 1994; 315: 161-169.

. Sallie R, Tredger JM and Williams R. Drugs and Liver. Biopharm. Drug Disposition. 1991; 12: 251-259.

. Azri S, Matta HP, Reid LL, Gandlofi AJ, Brendel K. Further examination of selective toxicity of CCl4 rat liver slices. Toxicol. Appl. Pharmacol. 1992; 112: 81-86.

.Kamalakkannan N, Rukkumani R, Aruna K, Varma PS, Viswanthan P, Padmanabhan V. Protective effect of N-acetyl cysteine in CCl4-induce hepatotoxicity in rats. Iranian J. Pharmacol. Thera. 2005; 4: 118-123.

. Singh B, Saxena AK, Chandan BK, Anand KK, Suri OP, Suri KA, Satti NK. Hepatoprotective activity of verbenalin on experimental liver damage in rodents. Fitoterap. 1998; 69:135–140.

. Clawson GA. Mechanism of carbon tetrachloride hepatotoxicity. Pathol. Immunopathol Res. 1989; 8: 104–112.

. Dubey GP, Agarwal A and Dixit SP. Effect of Liv-52 on different biochemical parameters in alcoholic cirrhosis. The Antiseptic, 1994; 91: 205– 208.

.Conney AH. Pharmacological implications of microsomal enzyme induction. Pharmacol. Rev. 1967; 19: 317-366.

. Javatilaka KAPW, Thabrew MI, Perera DJB. Effects of Maderaspatana on carbon tetrachloride-induced changes in the rat hepatic microsomal drug metabolozing enzyme activity. J. Ethnopharmacol. 1990; 30: 98-105.

. Castro JA, de Ferreyra GC, de Castro CR, Sesame H, de Fenos OM, Gillette JR. Prevention of carbon tetrachloride-induced necrosis by inhibitors of drug metabolism. Further studies on the metabolism of their action. Biochem. Pharmacol. 1974; 23: 295–302.

. Yasuda H, Izugami N, Shimadar O, Koba Yakawa Y, Nakanishi, M. The protective effect of tinoride against CCl4 hepatotoxicity. Toxicol. Appl. Pharmacol. 1980; 52: 407–413.

. La Casa CI, Villegas C, Alarcón de la Lastra V, Motilva M J, Martín C. Evidence for protective and antioxidant properties of rutin, a natural flavone against ethanol induced gastric lesions. J. Ethnopharmacol. 2000; 71: 45-53.

. Thabrew MI and Hughes RD. Phytogenic agents in therapy in liver diseases. Phytother. Res. 1996; 10: 461-467.


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