Phytochemical and antibacterial potentials of Tecoma stans and Costus afer

Ini Patrick Ekpe, Esua Orok Udosen

Abstract


Phytochemical composition and antibacterial potential of ethanolic extract of leaves/roots of Tecoma stans and leaves of Costus afer evaluated were collected at Malabo Campus, University of Calabar, Calabar and from Eman-Uruan local government area, Akwa Ibom, Nigeria respectively. Fresh leaves of both plants and roots of Tecoma stans collected were washed, dried on laboratory surface and milled into powder. They were separately extracted in 80% ethanol and the extracts concentrated in vacuo at 37-40oC to obtain semi solid dark green and brown concentrates respectively used for the evaluations. Phytochemical composition was determined using standard methods. The agar-well diffusion technique as described by Irobi et al, 1994 was employed for screening antibacterial activities. Five (5) bacterial isolates (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsilla pneumoniae , P.speceis, Shigella sonnei, Morganella morganii,and Maraxella catarrhlis were obtained from the University of Calabar Teaching Hospital, Calabar. They were maintained on nutrient agar at 4oC in the refrigerator.

The result showed that the leaves/roots of T.stans and leaves of C.afer contain Alkaloids, Flavonoids, Saponins and Glycosides. The MIC of leaves of T.stans showed 5.21mg/ml for S.aureus, 10.4mg/ml for E.coli, 83.3mg/ml for Proteus speies. The roots of T.stans showed 75mg/ml for S.aureus. The MIC for C.afer showed 5.2mg/ml for S. aureus and 312.5mg/ml for M. morganii. The results indicate that both plants can be used to achieve significant inhibitory effects as antimicrobial agents for treatment of bacterial infections in the absence of orthodox medication.


Keywords


Keywords: Antibacterial, natural products, nutrient broth, incubation, inhibition.

References


Adedapo AA., Mogbojuri OM., and. Emikpe BO Safety evaluations of the aqueous extract of the leaves of Moringa oleifera. Journal of Medicinal Plants Research, 2009a, 3(8), P. 586–591.

Burkill HM (1985). The useful plants of West Tropical Africa: Rev Dalziel’s J.M. Royal Botanical Gardens Kew. 2nd ed. Families A-D., 1: 135-191.

Christopher MR, Jamie BS., Jeffrey DK, David HS Natural Products Structural Diversity-I Secondary Metabolites: Organization and Biosynthesis in Comprehensive Natural Products II, 2010 453–492

Costantinol LA., Barlocco,D., Celotti, F., El-Abady SA., Baranetti T., Maggi R., Antolini L. Isolation and pharmacological activities of T.stans alkaloids. Pharmaco 2003;58(9) 781-785

Dahanukar SA., Kulkarni RA., Rege NN. 2000. Pharmacology of medicinal plants and natural products. Ind. J. Pharmacology; 32, S81-S118.

Eduardo Penton-Arias1, David DH in Immune Rebalancing, 2016 Immune Rebalancing The Future of Immunosuppression Academic, Pages 229–249 Academic Press

Edeoga HO, Okoli BE (2000). Chromosome numbers of Costus lucanusianus (Costaceae) in Nigeria. Folia Geobotanica 35: 315-318.

Govindappa M., Sadananda TS., Channabasava R., Jeevitha MK., Pooja KS and Vinay BR (2011). Journal of Phytology 3(3): 68-76 ISSN: 2075-6240 Phytopharmacology

Harvey AL. 2000.Strategies for discovering drugs from previously unexplored natural products. Drug Discov. Today, 5(7), 294-300.

Harvey AL. Natural products in drug discovery. Drug Discov. Today 13, 894–901 (2008).

Holt GA., Chandra A. 2002. Herbs in the modern healthcare environment- An overview of uses, legalities and the role of the healthcare professional. Clin. Res. Regulatory Affairs (USA), 19, 83-107.

Kudi AC., Myint SH. 1999. Antiviral activity of some Nigerian medicinal plant extracts. Journal of Ethnopharmacology 68, 289-294

Aguilar-Santamaria L., Ramirez G., Nicasio P., Alegria-Rayes C., Herrera-Arellano A. 2009. Antidiabetic activities of Tecoma stans (L.) Juss. ex Kunth. Journal of Ethnopharmacology 124, 284-288.

Nakanishi K.1999. An historical perspective of natural products chemistry. Comprehensive Nat. Prod. Chem., 8, xxi-xxxviii

Newman DJ., Cragg, Snader GM. 2012. Natural products as sources of new drugs over 30years from 1981-2010. Journal Natural Products, 75, 311-355

Nidhi M., Jain GC. Geeta P. 2010. Effect of Tecoma stans leaves on the reproductive system of male albino rats. International Journal of Pharmacology 6(20), 152-156

Odoemena, CS, Luke MI, Ubon, DG, Udotung, IR (2008). Journal of Research in Bioscience 4[1]:7-10.

Pavithra PS., Janani VS., Charumathi KH., Indumathy R., Sirisha P., Verma RS. 2010. Antibacterial activity of plants used in Indian herbal medicine. http://www.greenpharmacy May 14th

Soladoye M.O and. Oyesika O.O (2008) A Textbook of Medicinal Plants From Nigeria. University of Lagos Press. 628 pp.

Ramesh T ., Anusha V and Ravi KA (2009) Antibacterial Activity of Methanolic Extract of Roots of Tecoma stans Int. J. Chem. Sci.: 7(1), 6-8


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