Cardioprotective and antioxidant effects of Bougainvillea glabra against isoproterenol induced myocardial necrosis in albino rats

Rakam Gopi Krishna, Raja Sundara Rajan



The present study was executed to evaluate the myocardial protective effect of methanol extract of Bougainvillea glabra against isoproterenol induced myocardial necrosis in rats. Myocardial necrosis was induced by subcutaneous injection of isoproterenol (85mg/kg body weight) on 29th and 30th day at an interval of 24 hours. Myocardial necrosis was evident from the changes of marker enzymes in serum, plasma and heart tissue. The activities of serum cardiac marker enzymes such as lactate dehydrogenase (LDH), creatine kinase myoglobin (CK-MB), serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), triglycerides (TG), total cholesterol (TC), high density lipoproteins (HDL), low density lipoproteins (LDL), very low density lipoproteins (VLDL) and total protein (TP) were estimated. In addition, plasma TBARS and plasma LDH levels were also recorded. Antioxidant parameters viz catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), glutathione peroxidase (GPx) and malondialdehyde (MDA) levels were performed in heart tissue homogenate. The outcome of the study indicated that, pretreatment with methanol extract of Bougainvillea glabra to isoproterenol induced rats significantly prevented the altered serum cardiac marker enzymes, plasma levels and antioxidant parameters to near normal status. The cardioprotective effect was compared with propranolol (10 mg/kg, oral) which was used as the standard. Histopathological findings exposed a reduced degree of necrosis and inflammation succeeding pretreatment with Bougainvillea glabra.Based on these results, it was suggested that methanol extract of Bougainvillea glabra prevents myocardial necrosis and oxidative stress induced by isoproterenol.


Myocardial infarction, Bougainvillea glabra, biochemical parameters, propranolol, isoproterenol.

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[1] Rajalakshmy I, Ramya P, Kavimani S. Cardioprotective medicinal plants - A Review. Int. J. Pharm. Invent. 2011;1(1): 24-41.

[2] Hendriks EJ, Beulens JW, de Jong PA, van der Schouw YT, Sun WN, Wright CM, Criqui MH, Allison MA, Ix JH. Calcification of the splenic, iliac, and breast arteries and risk of all-cause and cardiovascular mortality. Atherosclerosis. 2017; 259:120-127

[3] Kosuge M, Kimura K, Ishikawa T. Differences between men and women in terms of clinical features of ST-segment elevation acute myocardial infarction". Circ.J. 2006; 70 (3): 222–226.

[4] Royja S. Sarcolemma membrane changes in myocardial necrosis induced by isoproterenol and salvage by drugs. New Delhi. 2013; 439

[5] Block G, Dietrich M, Norkus E P, Morrow JD, Hudes M, Cann B. Factors associated with oxidative stress in human populations. Am. J. Epidemiol. 2002; 156: 274-85.

[6] Kumar SV, Saritha G and Fareedullah M. Role of antioxidants and oxidative stress in cardiovascular diseases. Ann. Biol. Res. 2010; 1(3): 158–173.

[7] Nadkarni KM, Nadkarni AK: Indian Materia Medica, 3rd Ed., Popular Prakashan, Mumbai, 2005: 37-39.

[8] Raja S, Ramya I and Ravindranadh. K. A review on protective role of phytoconstituents against isoproterenol induced myocardial necrosis. Int. J. Pharmacog. Phytochem. Res. 2016; 8(5); 848-864

[9] Jancyrani JM, Chandramohan, G, Renganathan, R. Antioxidant Activity, preliminary phytochemical investigation and GC-MS Study of Bougainvillea glabra choicy Leaves. Int. J. Pharm. Pharm. Sci. 2013; 4(2): 0975-1491.

[10] Istieake Ahmed KM, Sabbir Ahmed O, Ahmed Abrar M, Al-Mamun Md, Tabibul Islam Md, Protiva Rani D, Mohammed R. Plant remedies of a Unani medicinal practitioner in Bhola district, Bangladesh. World J. Pharm. Pharm. Sci. 2015; 4: 186-198.

[11] Oudhia P. Traditional Medicinal Knowledge about Sthenias griastor Fb (Coleoptera: Cerambycidae) feeding on Bougainvillea spectabilis in Chattisgarh, India. 2002; 200.

[12] Grace I, Adebayo, Oluwakemi T, Alabi, Bamidele V, Owoyele and Ayodele O, Soladoye. Antidiabetic properties of the aqueous leaf extract of Bougainvillea glabra (Glory of the Garden) on Alloxan-Induced Diabetic Rats, Rec. Nat. Prod. 2009; 3(4): 187-192.

[13] Joshny J, Ramya Devi D, Vedha Hari BN. Phytochemical and in-vitro anthelmintic activity of hydro alcoholic extract of Bougainvillea glabra, Int. J. Pharm. Pharm. Sci. 2012; 4(2): 115-117.

[14] Amal Hussein A. New flavone from the aerial parts of Bougainvillea glabra. Int. J. Comput Engg. Res. 2014; 4(10): 2250-3005.

[15] Chandan BK, Saxena AK, Shukla S, Sharma N, Gupta DK, Suri KA, Suri J, Bhadauria M and Singh B. Hepatoprotective potential of Aloe barbadensis Mill. against carbon tetrachloride induced hepatotoxicity. J Ethnopharmacol, 2007; 111(3): 560–566.

[16] Moldeus P, Hogberg J, Orrenius S. Isolation and use of liver cells. Methods in Enzymology. 1978; 52: 55–62.

[17] Lamprecht W, Stan F, Weisser H and Heinz F. Determination of creatine phosphate and adenosine triphosphate with creatine kinase. In Methods of Enzymatic Analysis (ed. Bergmeyer, H. U.), Academic Press, NY, 1974; 1776–1778.

[18] Reitman S and Franke SA. A calorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminase. Am. J. Clin. Pathol. 1957; 28: 56–63

[19] Foster LB and Dunn RT. Stable reagents for determination of Serum triglycerides by a colorimetric Hantzsch condensation method. Clin Chem.1973; 196: 338 –340.

[20] Zlatkis A, Zak B and Boyle AJ. A new method for the direct determination of serum cholesterol. J. Lab. Clin. Med.1953; 41:486-92.

[21] Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low density lipoprotein cholesterol in plasma without the use of proparative centrifuge. J Clin Chem. 1972; 18: 499.

[22] Friedewald WT, Levy R, Fradrickson DS. Estimation of concentration of Lowdensity lipoprotein cholesterol in plasma without the use of preparative ultracentrifugation. Clin. Chem. 1972; 19: 449-452.

[23] Liu J, Edamatsu R, Kabuto H, Mori A. Antioxidant action of guilingji in the brain of rats with Fecl3-induced epilepsy. Free Radical Biol. Med. 1990; 9(5): 451-454.

[24] Lowry OH, Roseburgh NJ, Farr AL, Randal RJ: Protein measurement with the folin phenol reagent. J. Biol. Chem. 1951; 193: 265-275.

[25] Kakkar P, Das B, Viswanathan PN. A modified spectrophotometric assay of superoxide dismutase. Indian J. Biochem. Biophys. 1984; 21: 130-132.

[26] Aebi, H. Methods in enzymatic analysis, volume – 2. Catalase. In: Bergmeyer, H.V (Ed). Academic press, New Cork, Cheime, Weinheim, FRG, 1974. pp. 674-684.

[27] Ellman GL. Tissue sulphydryl groups. Arch. Biochem. Biophys. 1959; 82: 70–77.

[28] Paglia DE, Valentine WN. Studies on quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J. Lab. Clin. Med. 1967; 70: 158-169.

[29] Wendel, A. Glutathione peroxidase. Methods in Enzymology. 1981; 77: 325–333.

[30] Deepa PR and Varalakshmi P. Beneficial cardio-renovascular effects of a low molecular weight heparin derivative on adriamycin induced glycosamino glycanuria and tissue lipid abnormalities. Toxicology. 2005; 211: 77-85.

[31] Alcantara EH, Shin MY, Sohn HY, Park YM, Kim T, Lim JH, Jeong HJ, Kwon ST, Kwun IS. Diosgenin stimulates osteogenic activity by increasing bone matrix protein synthesis and bone-specific transcription factor Runx2 in osteoblastic MC3T3-E1 cells. J Nutr Biochem. 2011; 22(11):1055-1063.

[32] Barbara D, Domenica A, Giovanni S, Giuseppe MC, Mariarita A, Letteria M, Antonino S, Domenico C, Gioacchino C, Achille PC, Maria M and Francesco S. Cardioprotection by the phytoestrogen genistein in experimental myocardial ischaemia-reperfusion injury. Br. J. Pharmacol. 1999; 128: 1683–1690.

[33] Nigam, PK. Biochemical markers of myocardial injury. Indian J. Clin. Biochem. 2007; 22, 10–17.

[34] Rotenberg, Z.; Weinberger, I.; Sagle, A.; Fucha, J.; Sperling, O.; Agmon, J. Lactate dehydrogenase isoenzymes in serum during recent acute myocardial infarction. Clin. Chem. 1987; 33, 1419–1420.

[35] Paramasivam R, Dominic S, Chinthamony A, Velliyur KG. Cardioprotective effect of aqueous, ethanol and aqueous ethanol extract of Aerva lanata (Linn.) against doxorubicin induced cardiomyopathy in rats. Asian Pac. J. Trop. Biomed. 2012; S212-S218.

[36] Vijaya Padma V, Shyamala Devi CS, Ramkumar KM. Effect of fish oil pre-treatment on isoproterenol induced changes in myocardial membrane phospholipids. Nutrition. 2006; 22: 1171-1176.

[37] Ray, S., Bagchi, D., Lim, P.M., Bagchi, M., Gross, S.M., Kothari, S.C. , Preuss, H.G. and Stohs, S.J. Acute and long-term safety evaluation of a novel IH636 grape seed proanthocyanidin extract. Res. Commun Mol. Pathol. Pharmacol. 2001; 109: 165-197.

[38] Kurian GA, Philp S, Varghese T. Effect of aqueous extract of Desmodium gangeticum DC root in the severity of myocardial infarction. J Ethanopharmacol 2005; 97: 4557-461.


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