Antioxidant and antimicrobial activities of methanol leaf extract of Aegle Tamilnadensis Abdul Kader

M Chella Kumar, S Abdul Kader, C Sivaraj, N Raaman

DOI: http://dx.doi.org/10.5138/09750185.2157

Abstract


The present investigation was aimed to evaluate the phytochemical constituents, free radical scavenging activities, antioxidant properties and antimicrobial activities of the methanol leaf extract of Aegle tamilnadensis Abdul Kader. The phytochemicals present in the methanol leaf extract of A. tamilnadensis Abdul Kader were determined qualitatively and quantitatively using standard procedures. The antioxidant activities were carried out by DPPH free radical scavenging assay, OH radical scavenging assay, NOradical scavenging assay, Fe3+ reducing power assay, and phosphomolybdenum reduction assay methods. The antimicrobial activity was carried out by well diffusion method. The methanol leaf extract of A. tamilnadensis Abdul Kader showed good free radical scavenging as well as reducing power activities which were found to increase with the increasing concentration of the extract. The study revealed the presence of major phytochemicals such as phenols and flavonoids at the concentration of 211.0 mg/g at 100 µg and 52.91 mg/g at 100µg/mL respectively. The present study revealed that the methanol leaf extract of A. tamilnadensis Abdul Kader possess significant antioxidant and antimicrobial activities.

Keywords


Aegle tamilnadensis, phenols, flavonoids, antioxidant, antimicrobial.

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References


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[9]. Brand-williams W, Cuvelier ME and Berset C. Use of free radical method to evaluate antioxidant activity .Lebensmittel Wissenschaft and Technologie. 1995; 28(1): 25-30

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[14]. Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolke RH. “Manual of Clinical Microbiology”, 6th ed. Mosby Year Book, London.1995.

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[19]. Ulyana A, Daniel E, Michel H, Edward J, Kennelly S. Phytotherapy Res. 2002; 16 (3): 63-65.

[20]. Scott BC, Butler J, Halliwell B, Aruoma OI. Free radical activity of plant extracts. Free Rad. Res. Commun. 1993; 19(6): 241‐253.

[21]. Kunchandy E, Rao MNA. Oxygen radical scavenging activity of curcumin. Int J Pharmacol.1990; 58: 237‐40.

Abdul Kader S. Aegle tamilnadensis Abdul Kader – A New Record to Rutaceae. In: Proceedings of the International Conference on ‘Blooming Species– a Prosperous Terrain for Posterity’ held at Vellalar College for Women, Erode during. 2012;37.

Abdul Kader S. Occurence of New Aegle species (Aegle tamilnadensis Abdul Kader) in chennai ( The erstwhile Madras), Tamil Nadu, Southern India- An addition to the flora of Rutaceae. Journal of Economic and Taxonomic Botany. 2015;39(3-4):544-551.

Pratap Chandran R, Vysakhi MV, Manju S, Kannan M, Abdul Kader S. and Sreekumaran Nair A. In Vitro free radical scavenging activity of aqueous and methanolic leaf extracts of Aegle tamilnadensis Abdul Kader (Rutaceae). International Journal of Pharmacy and Pharmaceutical Sciences. 2013;5(3):819-823.

Raaman N, Phytochemical techniques. New India Publishing Agency, New Delhi. 2006;306.

Trease GE, Evans WC. Textbook of Pharmacognosy. 12th edn. Balliese Tindall and Company Publisher, London. 1983;343-383.

Harbome JB. Phytochemical Methods. Chapman and Hall Ltd. London. 1973;49-188.

Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent Methods Enzymol.1999;299:152-178.

Chang C, Yang M, Wen H, Chern J. Estimation of total flavonoids content in propolis by two complementary colorimetric methods. J. Food Drug Analysis. 2002;10:178-182.

Brand-williams W, Cuvelier ME and Berset C. Use of free radical method to evaluate antioxidant activity .Lebensmittel Wissenschaft and Technologie. 1995;28(1):25-30

Klenin SM, Cohen G, Cederbaum AI. Production of formaldehyde during metabolism of dimethyl sulphxide by hydroxyl radical generating system. Biochemistry. 1991;20:6006 6012.

Sreejayan Rao, Nitric MNA. Oxide scavenging by curcuminoids. Journal of Pharmacy and Pharmacology.1997;49:105-10.

Oyaizu M. Studies on product of browning reaction prepared from glucoseamine.Jpn J Nutr. 1986;07: 307‐15.

Prieto P, Pineda M, Anguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a Phosphomolybdenum Complex. Specific application to the determination of Vitamin E. Anal. Biochem. 1999;269:337-341.

Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolke RH. “Manual of Clinical Microbiology”, 6th ed. Mosby Year Book, London.1995.

Lopez-Gresa M, Ortiz R, Perello L, Latorre J, Liu-Gonzalez M, Garc´ıa-Granda S, Perez-Priede M, Canton E. Antibacterial studies journal of Inorganic Biochemistry.2002;92: 65.

Marcocci PL, Sckaki A, Albert GM. Antioxidant action of Ginkgo biloba extracts EGP761. Methods Enzymol.1994;234:462-475.

Rice-Evans CA, Miller NJ, Bolwell PG, BramLey PM, Pridham JB. The relative antioxidant activities of plant derived polyphenolic flavonoids. Free Radic.Res.1995; 22:375-383.

Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958; 29:1199-1200.

Ulyana A, Daniel E, Michel H, Edward J, Kennelly S. Phytotherapy Res. 2002;16(3):63-65.

Scott BC, Butler J, Halliwell B, Aruoma OI. Free radical activity of plant extracts. Free Rad. Res. Commun. 1993;19(6):241‐253.

Kunchandy E, Rao MNA. Oxygen radical scavenging activity of curcumin. Int J Pharmacol.1990; 58:237‐40.


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