Sodium silicate mediated response of antioxidative defense system in Lycopersicon esculentum mill. under water stress

Chanchal Malhotra, Riti Thapar Kapoor, Deepak Ganjewala, NB Singh



The present study was designed to study the effect of water stress on Lycopersicon esculentum Mill. and role of sodium silicate in the protection of tomato plants under water deficit condition.

Different biochemical parameters such as photosynthetic pigments, protein, sugar, MDA content, proline, nitrate reductase activity and activities of antioxidant enzymes (SOD, CAT, APX and POX) were examined in tomato leaves at 40 and 60 DAS by the standard methods. The lycopene and β-carotene contentsin tomato fruits were also analyzed at 60, 65 and 70 DAS.

Water stress significantly decreased relative water content (RWC), pigment content, sugar and protein contents in tomato leaves at 60 DAS but the accumulation of proline was stimulated in tomato leaves under water deficit condition. The activities of antioxidant enzymes such as SOD, CAT, APX and POX were significantly increased under (3d and 6d) water stress condition at 60 DAS.

This study offers first hand information on the water stress-induced oxidative stress in Lycopersicon esculentumand development of antioxidative defense system against drought.The results obtained clearly indicated the positive impact of sodium silicate in protection of tomato plants under water deficit condition.

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[1]. Lipiec J, Doussan C, Nosalewicz A. Kondracka K. Effect of drought and heat stresses on plant growth and yield: A review. Int. Agrophys. 2013; 27:463-477.

[2]. Akram H M, Ali A, Sattar A, Rehman HSU, Bibi A. Impact of water deficit stress on various physiological and agronomic traits of three Basmati rice (Oryza sativa L) cultivars. J. Anim. Plant Sci. 2013;23(5): 1415-1423.

[3]. Sikuku PA, Onyango JC, Netondo GW. Yield components and gas exchange responses of Nerica rice varieties (Oryza sativa L.) to vegetative and reproductive stage water deficit. Global J. Sci. Frontier Res.2012; 12: 51-62.

[4]. Ben Ahmed C, Ben Rouina N, Sensory F, Boukhris M, Ben Abdalah F. Changes in gas exchanges, proline accumulation and antioxidative enzyme activity in three olive cultivars under contrasting water availability regimes. Environ Exp. Bot. 2009; 67: 347-352.

[5]. Yordanov I, Velikova V, Tsonev T. Plant responses to drought, acclimationand stress tolerance. Photosynthetica J. 2000; 38(2): 171-186.

[6]. Mittler R. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci. 2002; 7: 405-410.

[7]. Azooz MM, Al-Fredan M A. The inductive role of vitamin C and its mode of application on growth, water status, antioxidant enzyme activities and protein patterns of Viciafaba L. cv. Hassawi grown under seawater irrigation.Am. J. Plant Physiol. 2009; 4: 38-51.

[8]. An YY, Liang ZS. Drought tolerance of Periplocasepium during seed germination: antioxidant defense and compatible solutes accumulation. ActaPhysiol Plant. 2013; 35: 959-967.

[9]. Mittler R, Vanderauwera S, Gollery M, Van Breusegem F. Reactive oxygen gene network of plants. Trends in Plant Science . 2004; 9: 490-498.

[10]. Wang WB, Kim YH, Lee H S, Kim K Y, Deng X P, Kwak SS. Analysis of antioxidant enzyme activity during germination of alfalfa under salt and drought stress. Plant Physiol. Biochem. 2009;47: 570-577.

[11]. Frusciante L, Carli P, Ercolano MR, Pernice R, Di Matteo A, Fogliano V, Pellegrini N. Antioxidant nutritional quality of tomato. Mol. Nutr. Food Res.2007; 51, 609-617.

[12]. Clinton SK. Lycopene: chemistry, biology, and implications for human health and disease. Nutr. Rev.1998; 56: 35-51.

[13]. Paiva SA, Russell RM. Beta-carotene and other carotenoids as antioxidants. Journal of the American College of Nutrition. 1999; 18: 426-433.

[14]. Krauss S, Schniztler WH, Grassmann J, Woitke M. The influence of different electrical conductivity values in a simplified recirculating soilless system on inner and outer fruit quality characteristics of tomato. J Agric Food Chem. 2006; 54:441- 448.

[15]. Sies W, Stahl H. 2003.Carotenoids and flavonoids contribute to nutritional protection against skin damage from sunlight.MolBiotechnol. 2007;37(1):26-30.

[16]. Hasanuzzaman M, Nahar K, Alam MM, Roychowdhury R, Fujita M. Physiological, biochemical, and molecular mechanisms of heat stress tolerance in plants. Int. J. Mol. Sci. 2013; 14: 9643-9684.

[17]. Sawas D, Ntatsi G. Biostimulant activity of silicon in horticulture. Sci. Hortic. 2015; 196: 66-81.

[18]. Hakim Y, Khan AL, Shinwari ZK, Kim D, Waqas M, Lee I. Silicon treatment to rice (Oryza sativa L.) plants during different growth periods and its effects on growth and grain yield. Pak. J. Bot. 2012; 44: 891-897.

[19]. Bokhtiar SM, Huang H, Li Y, Dalvi VA. Effects of silicon on yield contributing parameters and its accumulation in abaxial epidermis of sugarcane leaf blades using energy dispersive x-ray analysis. J. Plant Nutr. 2012; 35: 1255-1275.

[20]. Tahir MA, Aziz RT, Ashraf M, Kanwal S, Maqsood MA. Beneficial effects of silicon in wheat (Triticumaestivum L.) under salinity stress. Pak. J. Bot. 2006; 38(5): 1715-1722.

[21]. Ahmed M, FayyazUl H, Qadeer U, Aslam MA. Silicon application and drought tolerance mechanism of sorghum. Afr. J. Agr. Res. 2011;6 :594-607.

[22]. Shen X, Zhou Y, Duan L, Li Z, Eneji AE,Li J. Silicon effects on photosynthesis and antioxidant parameters of soybean seedlings under drought and ultraviolet-B radiation. J. Plant Physiol. 2010;167: 1248–1252.

[23]. Guo Z, Ou W, Lu S, Zhong Q. Differential responses of antioxidative system to chilling and drought in four rice cultivars differing in sensitivity. Plant Physiology and Biochemistry. 2006; 44: 828-836.

[24]. Bor M, Ozdemir F, Turkan I. The effect of salt stress on lipid peroxidation and antioxidants in leaves of sugar beet Beta vulgaris L. and wild beet Beta maritima L. Plant Science. 2003;164: 77-84.

[25]. Khanna-Chopra R, Selote DS. Acclimation to drought stress generates oxidative stress tolerance in drought-resistant than -susceptible wheat cultivar under field conditions. Environmental and Experimental Botany. 2007; 60: 276-283.

[26]. Manivannan P, Abdul Jaleel C, Kishorekumar A, Sankar B, Somasundaram R, Sridharan R, Panneerselvam R. Changes in antioxidant metabolism of Vignaunguiculata L.Walp. bypropiconazole under water deficit stress. Colloids and Surfaces Biointerfaces, 2007; 57: 69-74.

[27]. Tohidi-Moghadam HR, Shirani-Rad AH, Nour-Mohammadi G, Habibi D, Mashhadi-Akbar-Boojar M. Effect of super absorbent application on antioxidant enzyme activities in canola (Brassica napus L.) cultivars under water stress conditions. Am. J. Agric. Biol. Sci. 2009; 4(3): 215-223.

[28]. Barrs HD, Weatherley PE. A re-examination of the relative turgidity technique for estimating water deficits in leaves. Australian J of Biol. Sci. 1962; 15: 413-428.

[29]. Lichtenthaler HK. Chlorophylls and carotenoids: pigment photosynthetic biomembranes. Methods in Enzymology. 1987; 148: 362-385.

[30]. Sairam RK, Deshmukh PS, Shukla DS. Tolerance of drought and temperature stress in relation to increased antioxidant enzyme activity in wheat. Journal of Agronomy and Crop Science. 1997; 178: 171-178.

[31]. Heath R L, Packer L. Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics. 1968; 125:189-198.

[32]. Hedge JE, Hofreiter BT. Estimation of carbohydrate. In: Whistler, R.L., Be Miller, J.N., (eds.), Methods in Carbohydrate chemistry. Academic Press, New York. 1962; 17-22.

[33]. Lowry O, Rosebrough A, Far A, Randall R. Protein measurement with folin phenol reagent. J. Biol. Chem. 1951; 193: 680-685.

[34]. Jaworski EG. Nitrate reductase assay in intact plant tissues. BiochemBiophys. Res. Comm. 1971; 43:1274-1279.

[35]. Bates L, Waldren R, Teare J. Rapid determination of proline for water stress studies// Plant Soil. 1973; 39: 205-207.

[36]. Concepcion RM, Gruissem W. Arachidonic acid alters tomato HMG expression and fruit growth and induces 3-hydroxy-3-methylglutaryl coenzyme A reductase-independent lycopene accumulation. Plant Physiology. 1999; 119: 41-48.

[37]. Nagata M, Yamashita I. Simple method for simultaneous determination of chlorophyll and carotenoids in tomato fruit. Nippon Shokuhin Kogyo Gakkaish. 1992; 39(10): 925-928.

[38]. Beyer WF, Fridovich I. Assaying for superoxide dismutase activity:some large consequences of minor changes in condition. Anal. Biochem. 1987; 161: 559-566.

[39]. Cakmak I, Marschner H. Magnesium deficiency and highlight intensity enhance activities of superoxide dismutase ascorbate peroxidase, and glutathione reductase in bean leaves. Plant Physiol. 1992; 98: 1222-1227.

[40]. Mc Cune, Galston A W. Inverse effects of gibberellin on peroxidase activity and growth in dwarf strains of peas and corn'. Plant Physiol. 1989; 34: 416-418.

[41]. Nakano Y, Asada K. Purification of ascorbate peroxidase in spinach chloroplasts: its inactivation in ascorbate‐depleted medium and reactivation by monodehydroascorbate radical. Plant Cell Physiology. 1987; 28: 131-140.

[42]. Malhotra C, Kapoor RT, Ganjewala D. Protective role of sodium silicate against water stress in Lycopersiconesculentum Mill. International Journal of Pharma and Bio Sciences. 2016; 7(4): 909- 917.

[43]. Romero-Aranda MR, Jurado O, Cuartero J. Silicon alleviates the deleterious salt effect on tomato plant growth by improving plant water status. J. Plant Physiol.2006; 163: 847-855.

[44]. Zhu ZJ, Wei GQ, Li J, Qian QQ, Yu JP. Silicon alleviates salt stress and increases antioxidant enzymes activity in leaves of salt-stressed cucumber (Cucumissativus L.). Plant Sci. 2004; 167:527-533.

[45]. Shu LZ, Liu YH. Effects of silicon on growth of maize seedlings under salt stress. Agroenviron Prot. 2001; 20:38-40.

[46]. Epstein E. Silicon: its manifold roles in plants. Ann. Appl. Biol.2009; 155: 155-160.

[47]. Sonobe K, Hattori T, An P, Tsuji W, Eneji AE, Kobayashi S, Kawamura Y, Tanaka K, Inanaga S. Effect of silicon application on Sorghum root responses to water stress. J. Plant Nutr. 2011; 34: 71-82.

[48]. Kamel A, Loser DM. Contribution of carbohydrates and other solutes to osmotic adjustment in wheat leaves under water stress. J. Plant Physiol. 1995;145: 363-366.

[49]. Hussain M, Malik MA, Farooq M, Ashraf MY, Cheema MA. Improving drought tolerance by exogenous application of glycine betaine and salicylic acid in sunflower. J. Agron. Crop Sci. 2008; 194: 193-199.

[50]. Wullschleger SD, Yin TM, DiFazio SP, Tschaplinski TJ, Gunter LE, Davis MF, Tuskan GA. Phenotypic variation in growth and biomass distribution for two advanced-generation pedigrees of hybrid poplar. Canadian J. For. Res.2005; 35:1779-1789.

[51]. Farooq M, Basra SMA, Wahid A, Rehman H. Exogenously nitric oxide enhances the drought tolerance in fine grain aromatic rice (Oryza sativa L.). Journal Agronomy & Crop Science. 2009; 195:254-261.

[52]. Savvas D, Giotis D, Chatzieustration E, Bakea M, Patakioutas G. Silicon supply in soil less cultivations of Zucchini alleviates stress induced by salinity and powdery mildew infections. Environmental Experimental Botany.2009; 65: 11-17.

[53]. Pei ZF, Ming DF, Liu D, Wan GL, Geng XX, Gong HJ, Zhou WJ. Silicon improves the tolerance to water-deficit stress induced by polyethylene glycol in wheat (Triticumaestivum L.) seedlings. J Plant Growth Regul. 2010; 29:106-115.

[54]. Jiang Y, Huang B. Drought and heat injury to two cool-season turf grasses in relation to antioxidant metabolism and lipid peroxidation. Crop Science. 2001; 41: 436-442.

[55]. Blokhina O, Virolainen E, Fagerstedt KV. Antioxidants, oxidative damage and oxygen deprivation stress: a review. Annals of Botany. 2003; 91: 179-194.

[56]. Oracz K, Bailly C, Gniazdowska A, Coˆme D, Corbineau F, Bogatek R. Induction of oxidative stress by sunflower phytotoxins in germinating mustard seeds. J. Chem. Ecol. 2007; 33: 251-264.

[57]. Rahman MU, Gul S, Ahmad I. Effects of water stress on growth and photosynthetic pigments of corn (Zea mays L.) cultivars. International Journal of Agriculture and Biology. 2004; 6(4): 652-655.

[58]. Schmidt RE, Zhang X, Chalmers D R. Response of photosynthesis and superoxide dismutase to silica applied to creeping bentgrass grown under two fertility levels. Journal of Plant Nutrition. 1999; 22(11):1763-1773.

[59]. Koleyoreas SA. A new method for determining drought resistance. Plant Physiol. 1958; 33: 232-233.

[60]. Kiani SP, Maury P, Sarrafi A, Grieu P. QTL analysis of chlorophyll fluorescence parameters in sunflower (Helianthus annuus L.) under well-watered and water-stressed conditions. Plant Sci. 2008;175: 565-573.

[61]. Tahkokorpi M, Taulavuori K, Laine K, Taulavuori E. Aftereffects of drought-related winter stress in previous and current year stems of Vacciniummyrtillus L. Environ. Exp. Bot. 2007; 61: 85-93.

[62]. Perks JP, Monaghan S, O’Reilly C, Osborne BA, Mitchell DT (2001). Chlorophyll fluorescence characteristics, performance and survival of freshly lifted and cold stored Douglas fir seedlings. Ann Forest Sci. 58: 225-235.

[63]. Naumann JC, Bissett SN, Young DR, Edwards J, Anderson JE. Diurnal patterns of photosynthesis, chlorophyll fluorescence and PRI to evaluate water stress in the invasive species, Elaeagnusumbellata Hub. Trees. 2010; 24: 237-245.

[64]. Ohashi Y, Nakayama N, Saneoka H, Fujita K. Effects of drought stress on photosynthetic gas exchange, chlorophyll fluorescence and stem diameter of soybean plants. BiologiaPlantarum. 2006; 50: 138-141.

[65]. De Ell JR,Toivonen PMA. Use of chlorophyll fluorescence in postharvest quality assessments of fruits and vegetables. In: Practical Applications of Chlorophyll Fluorescence in Plant Biology, (eds. J.R. De Ell and P.M.A.)Toivonen, Boston: Kluwer Academic Publishers. 2003; 201-242.

[66]. Chen ML, Yang DJ, Liu SC. Effects of drying temperature on the flavonoid, phenolic acid and antioxidative capacities of the methanol extract of citrus fruit (Citrus sinensisL.) peels. Int J Food Sci Technol. 2011;46:1179-1185.

[67]. Tale-Ahmad S, Haddad R. Study of silicon effects on antioxidant enzyme activities and osmotic adjustment of wheat under drought stress. Czech J. Genet. Plant Breed.2011; 47: 17-27.

[68]. Gong H J, Zhu XY, Chen K M, Wang S M, Zhang C L. Silicon alleviates oxidative damage of wheat plants in pots under drought. Plant Sci. 2005; 169: 313-321.

[69]. Kojo S. Vitamin C: basic metabolism and its function as an index of oxidative stress. Curr Med Chem. 2004;11:1041-1064.

[70]. Hessini K, Martínez JP, Gandour M, Albouchi A, Soltani A, Abdelly C. Effect of water stress on growth, osmotic adjustment, cell wall elasticity and water-use efficiency in Spartinaalterniflora. Environ. Exp. Bot. 2009;67:312-319.

[71]. KaviKishor PB, Sreenivasulu N. Is proline accumulation per se correlated with stress tolerance or is proline homeostasis a more critical issue? Plant Cell Environ. 2014; 37(2): 300-311.

[72]. Reddy AR, Chaitanya KV, Jutur PP, Sumithra K. Differential antioxidative responses to water stress among five mulberry (Morusalba L.) cultivars. Environ. Exp. Bot. 2004;52: 33-42.

[73]. Ali Q, Ashraf M, Athar H. Exogenously applied proline at different growth stages enhances growth of two maize cultivars grown under water deficit conditions. Pakistan Journal of Botany. 2007; 39(4):1133-1144.

[74]. Fariduddin Q, Yusuf M, Hayat S, Ahmad A. Effect of 28-homobrassinolide on antioxidant capacity and photosynthesis in Brassica juncea plants exposed to different levels of copper. Environ. Exp. Bot. 2009; 66: 418-424.

[75]. Becker TW, Fock HP. Effects of water stress on the gas exchange, the activities of some enzymes of carbon and nitrogen metabolism and on the pool sizes of some organic acids in maize leaves. Photosynthesis Research. 1986; 8:175-181.

[76]. Dhindsa RS, Cleland RE. Water stress and protein synthesis: I. Differential inhibition of protein synthesis. Plant Physiol. 1975; 55(4):778-781.

[77]. Davies WJ, Van Volkenburgh E. Influence of water deficit on the factors controlling the daily pattern of growth of Phaseolus vulgaristrifoliates. Journal of Experimental Botanv. 1983; 34: 987-999.

[78]. John R, Ahmad P, Gadgill K, Sharma S. Effect of cadmium and lead on growth, biochemical parameters and uptake in Lemnapolyrrhiza L. Plant Soil Environ. 2008; 54: 262–270.

[79]. Elsheery NI, Cao KF. Gas exchange chlorophyll fluorescence and osmotic adjustment in two mango cultivars under drought stress. Acta Physiol. Plant. 2008; 30:769-777.

[80]. Choluj D, Karwowska R, Ciszewska A, Jasinska M. 2008. Influence of long-term ´ drought stress on osmolyte accumulation in sugar beet (Beta vulgaris L.) plants. Acta Physiol. Plant. 2008; 30: 679-687.

[81]. Regier N, Streb S, Cocozza C, Schaub M, Cherubini P, Zeeman SC, Rrey B. Drought tolerance of two black poplar (Populusnigra L.) clones: contribution of carbohydrates and oxidative stress defence. Plant Cell Environ. 2009; 32: 1724-1736.

[82]. Rahman MA, Mossa JS, Al-Said MS, Al-Yahya MA. Medicinal Plant diversity in the flora of Saudi Arabia 1: a repirt on seven plantfamilies. Fitoterpia 2004; 75: 149-161.

[83]. Stewart CR, Boggess SF, Aspinall D, Paleg LG. Inhibition of proline oxidation by water stress. Plant Physiol. 1977; 59: 930-932

[84]. Hsiao TC. Plant responses to water stress. Annu. Rev. Plant Physiol. 1973; 24: 519-570.

[85]. Foyer CH, Valadier M-H, Migge A, Becker TW. Drought-induced effects on nitrate reductase activity and mRNA and on the coordination of nitrogen and carbon metabolism in maize leaves. Plant Physiology.1998; 117: 283-292.

[86]. Singh NB, Yadav K, Amist N. Positive effects of nitric oxide on Solanum lycopersicum. J. Plant Inter. Actions. 2014; 9: 10-18.

[87]. Chen CL, Sung JM. The effect of water stress on enzymes of carbon metabolism in the cytosol of soybean nodules. Journal of the Agricultural Association of China. 1983; 121:28–34.

[88]. Suzuki N, Mittler R. Reactive oxygen species and temperature stresses: a delicate balance between signalling and destruction. Physiol. Planta. 2006; 126:45-51.

[89]. Foyer CH, Noctor G. Redox sensing and signalling associated with reactive oxygen in chloroplasts, peroxisomes and mitochondria. PhysiologiaPlantarum. 2003; 119(3): 355-364.

[90]. Ghahfarokhi MG, Mansurifar S, Taghizadeh-Mehrjardi R, Saeidi M, Jamshidi A M, Ghasemi E. Effects of drought stress and rewatering on antioxidant systems and relative water content in different growth stages of maize hybrids. Archives of Agronomy and Soil Science. 2015; 61: 493-506.

[91]. Asada K. The water-water cycle in chloroplasts: scavenging of active oxygens and dissipation of excess photons. Annu Rev Plant Physiol Plant Mol Biol. 1999; 50: 601-639.

[92]. Gomez LD, Noctor G, Knight M, Foyer CH. Regulation of calcium signalling and gene expression by glutathione. J Exp Bot. 2004;55:1851-1859.

[93]. Noctor G, Foyer CH. Ascorbate and glutathione: keeping active oxygen under control. Annual Review of Plant Physiology and Plant Molecular Biology. 1998; 49: 249-279.

[94]. Shao HB, Chuc LY, Wu G, Zhang JH, Lua ZH, Hug YC. Changes of some anti-oxidative physiological indices under soil water deficits among 10 wheat (Triticumaestivum L.) genotypes at tillering stage. Colloids Surf B Biointerfaces. 2007;54:143-9.

[95]. Abdul Jaleel C, Sankar B, Murali PV, Gomathinayagam M, Lakshmanan G M A, Panneerselvam R. Water deficit stress effects on reactive oxygen metabolism in Catharanthusroseus; Impacts on ajmalicine accumulation. Colloids Surface.2008; 62:105-111.

[96]. Wang WB, Kim YH, Lee HS, Kim KY, Deng XP, Kwak SS. Analysis of antioxidant

enzyme activity during germination of alfalfa under salt and drought stresses. Plant PhysiolBioch 2009;47:570-577.

[97]. Akcay UC, Ercan O, Kavas M, Yildiz L, Yilmaz C, Oktem HA, Yucel M. Drought-induced oxidative damage and antioxidant responses in peanut (Arachishypogaea L.) seedlings. Plant Growth Regul. 2010; 61: 21-28.

[98]. Omidi H. Changes of proline content and activity of antioxidative enzymes in two canola genotype under drought stress. Am. J. Plant Physiol.2010; 5: 338-349.

[99]. Romero-Romero T, Sa´nchez-Nieto S, SanjuanBadillo A, Anaya AL, Cruz-Ortega R. Comparative effects of allelochemical and water stress in roots of Lycopersiconesculentum Mill. plant. Plant Sci.2005; 168: 1059-1066.

[100]. Oracaz K, Bailly C, Gniazdowska A, Côme D, Corbineau F, Bogatek R. Induction of oxidative stress by sunflower phytotoxins in germinating mustard seeds'. J of Chemi Ecol. 2007; 33:251-264.

[101]. Vasconcelos ACF, Zhang X, Ervin EH, Kiehl JC. Enzymatic antioxidant responses to biostimulants in maize and soybean subjected to drought. Scientia Agricola. 2009; 66: 395-402.

[102]. Upadhyaya H, Panda SK, Dutta BK. Variation of physiological and antioxidative responses in tea cultivars subjected to elevated water stress followed by rehydration recovery. Acta Physiol. Plant. 2008; 30: 457-468.


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