Evaluation of hepatoprotective effect of Waltheria indica against various NSAIDs-induced hepatic damage in rats

Amol Chandekar, Neeraj Upamanyu, Amber Vyas, Atul Tripathi, Surendra Agrawal, Deenanath Jhade

DOI: http://dx.doi.org/10.5138/09750185.2081

Abstract


The objective of the present study was to evaluate methanolic extract of leaves of Waltheria indica linn. for hepatoprotective potency of the potent solvent extract. The hepatotoxicity was induced by diclofenac, carbon tetrachloride (CCl 4 ) and acetaminophen. In CCl 4 induced hepatotoxicity study, animals were divided into five groups (n=6). Methanolic extract of Waltheria indica (WIM) groups were injected in doses of 400 mg/kg and 600mg/kg body weight along with CCl 4 and Silymarin 100mg/kg was taken as standard drug. Similarly procedure was followed in diclofenac and acetaminophen induced hepatotoxicity. Blood samples and liver were collected and liver hisopathological studies were carried out. These histopathological analysis suggested that WIM extract have the ability to reduce the degree of hepatic fibrosis induced by various factors. And concluded that WIM extract has significant hepatoprotective activity thus this study scientifically support the theory to use of this plant in traditional medicine for the treatment of liver disorders.


Keywords


Diclofenac, Carbon tetrachloride (CCl 4 ) and Acetaminophen, Waltheria indica methanolic extract (WIM), hepatoprotective activity

Full Text:

PDF

References


[1]. Saleem TSM, Chetty CM, Ramkanth S, Rajan VST, Kumar KM, Gauthaman K. Hepatoprotective herbs - a review. International Journal of Research in Pharmaceutical Sciences 2010;1: 1-5.

[2]. Ajith TA, Hema U, Aswathy MS. Zingiber officinale Roscoe prevents acetaminophen-induced acute hepatotoxicity by enhancing hepatic antioxidant status. Food and Chemical Toxicology 2007;45: 2267-2272.

[3]. Pandit A, Sachdeva, T. and Bafna, P., 2012. Drug-induced hepatotoxicity: A review.

[4]. Bessone F, Non-steroidal anti-inflammatory drugs: What is the actual risk of liver damage. World J Gastroenterol 2010;16(45),5651-61.

[5]. Tanna A, Nair R, Chanda S. Assessment of anti-inflammatory and hepatoprotective potency of Polyalthia longifolia var. pendula leaf in Wistar albino rats. Journal of Natural Medicines 2009;63: 80-85.138

[6]. Jain SC, Jain R, Singh R. Ethnobotanical survey of Sariska and Siliserh regions from Alwar district of Rajasthan, India. Ethnobotanical Leaflets 2009;13:171–188.

[7]. Ajith TA, Hema U, Aswathy MS. Zingiber officinale Roscoe prevents acetaminophen-induced acute hepatotoxicity by enhancing hepatic antioxidant status. Food and Chemical Toxicology 2007;45: 2267-2272.

[8]. Yatzidis H. New colorimetric method for quantitative determination of protein in urine. Clinical chemistry, 1977; 23(5):811-812.

[9]. Corcoran RM and Durnan SM. Albumin determination by a modified bromcresol green method. Clinical chemistry 1977;23(4): 765.

[10]. Fawcett JK and Scott J. A rapid and precise method for the determination of urea. Journal of clinical pathology, 1960; 13(2): 156-159.

[11]. Kind PRN and King EJ. Estimation of plasma phosphatase by determination of hydrolysed phenol with amino-antipyrine. Journal of clinical Pathology, 1954; 7(4): 322.

[12]. Reitman S and Frankel S. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. American journal of clinical pathology, 1957; 28(1): 56-63.

[13]. Lowry OH, Rosebrough NJ, Farr AL and Randall RJ. Protein measurement with the Folin phenol reagent. J biol Chem, 1951; 193(1): 265-275.

[14]. Ellman GL. Tissue sulfhydryl groups. Archives of biochemistry and biophysics, 1959;82(1): 70-77.

[15]. Sinha AK, Colorimetric assay of catalase. Analytical biochemistry, 1972: 47(2): 389-394.

[16]. Mills GC. The purification and properties of glutathione peroxidase of erythrocytes. Journal of Biological Chemistry, 1959; 234(3): 502-506.

[17]. Raghuramulu N, Nair KM and Kalyanasundaram S. eds. A manual of laboratory techniques. National Institute of Nutrition, Indian Council of Medical Research 1983.


Refbacks

  • There are currently no refbacks.
';



Copyright (c) 2017 Amol Chandekar, Amber Vyas, Neeraj Upamanyu, Atul Tripathi, Surendra Agrawal, Deenanath Jhade

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Advanced Research Journals

18K, Street 1st, Gaytri Vihar, Pinto Park, Gwalior, M.P. India

Copyright@arjournals.org (Design) 2009-2017

 

Follow @arjournals on Twitter