Oral supplementations of Bauhinia variegata’s leaf extract has anxiolytic effect and improves locomotory and exploratory behavior of female albino mice

Furhan Iqbal, Muhammad Jahangir, Asmat Ullah, Muhammad Naseer Khan Khattak, Shahid Iqbal

DOI: http://dx.doi.org/10.5138/09750185.2073

Abstract


Present study was designed to investigate the effects of 200 mg/ml solvent/Kg of body weight of Bauhinia variegata’s leaf extract on neuromuscular coordination, locomotory and exploratory behavior of adult female albino mice. Six week old female albino mice were used as experimental animals. Mice were either orally supplemented with 200 mg/ml/Kg of body weight Bauhinia variegata’s leaf extract or with saline solution [0.9%] for 7 days. Behavioral observations were made by applying a series of neurological tests [Rota rod, Elevated plus maze, Light and dark box, Open field and Novel object test]. Exploratory and locomotory behavior during Open Field test was improved in leaf extract treated mice as they had more means speed [P = 0.01], rotations [P = 0.001], clockwise rotations [P = 0.004] and anti-clockwise rotations [P = 0.001] than control mice. Bauhinia variegata’s leaf extract treated mice had higher transition frequency [P = 0.002] and spent more time in light compartment during light dark box test than saline treated controls. Bauhinia variegata’s leaf extract treated mice covered more distance [P = 0.033] with higher speed [P = 0.028] compared to control mice during Elevated Plus Maze test. Bauhinia variegata’s leaf extract improves the locomotory and exploratory behavior in adult female albino mice. Leaf extract of Bauhinia variegate can be further explored to be used as a therapeutic agent for the treatment of neurological ailments.


Keywords


Bauhinia variegata; open field test; rota rod test; novel object test; elevated plus maze; female albino mice

References


- Nader K. Memory traces unbound. Trend Neurosci. 2003; 26: 65-72. 2- Vanderwolf CH, Cain DP. The behavioural neurobiology of learning and memory: a conceptual reorientation. Brain Res Rev. 1994; 19: 264-297. 3- Ikarashi Y, Iizuka S, Imamura S. Effects of yokukansan, a traditional Japanese medicine, on memory disturbance and behavioural and psychological symptoms of dementia in thiamine-deficient rats. Biol Pharma Bull. 2009; 32: 1701-1709. 4- Kim DH, Yoon BH, Kim YW, Lee S, Shin BY, Jung JW, Kim HJ, Lee YS, Choi JS, Kim SY, Lee KT,Ryu JH. The seed extract of Cassia obtusifolia ameliorates learning and memory impairments induced by scopolamine or transient cerebral hypoperfusion in mice. J Pharma Sci. 2007; 105: 82-93. 5- Zahra K, Khan MA, Iqbal F. Oral supplementation of Ocimum basilicum enhances the locomotory, exploratory and learning behaviour of adult male albino mice. Neurol Sci. 2015; 236910: 73-78. 6- Amrani S, Harnafi H, Gadi, Mekhfi H, Legssyer A, Aziz M, Martin-Nizard F, Bosca, L.Vasorelaxant and anti-platelet aggregation effects of aqueous Ocimum basilicum extract. J Ethnopharmacol. 2009; 125: 157–62. 7- Sahu G, Gupta PK. A Review of Bauhinia variegata Linn. International Research J Pharma. 2012; 3: 48-51. 8- Abd EW, Wassel SM, Ammar GM, Hanna NM. Flavonoids constituents in different organs of selected Bauhinia species and their effect on blood glucose. Herba Hungar. 1987; 26: 27-39. 9- Yadava RN, Madhusudhan R. Anti-inflammatory activity of a novelflavonol glycosides from Bauhinia variegata Linn. Nat Prod Res. 2002; 17: 165-169. 10- Rajkapoor B, Jayakar B, Murugesh N. Antitumor activity of Bauhinia variegata on Dalton’s ascetic lymphoma. J Ethnopharmacol. 2003; 89: 107-109. 11- Rajkapoor B, Jayakar B, Murugesh N, Sakthisekaran D. Chemopreventive and cytotoxic effect of Bauhinia variegata against N-nitrosodiethylamine induced liver tumors and human cancer cell lines. J Ethnopharmacol. 2006; 104: 407-409. 12- Sunyer B, Patil S, Frischer C, Hoger H, Selcher J, Brannath W,Lubec G.Strain dependent effects of SGS742 in the mouse. Behav Brain Res. 2007; 18: 164-175. 13- Bourin M, Hascoët M.The mouse light/dark box test. Eur J Pharmacol. 2003; 463: 55-65. 14- Prut L, Belzung C. The open field as a paradigm to measure the effects of drugs on anxiety-like behaviors: a review. Eur J Pharmacol. 2003; 463: 3-33. 15- Weitzdoerfer R, Hoegar H, Engidawork E, Engelmann M, Singewald N,Lubec G. Neuronal nitric oxide synthase knock - out mice show impaired cognitive performance. Nitric Oxide. 2004; 10: 130-140. 16- Walf AA, Frye CA [2007] The use of the elevated plus maze as an assay of anxiety-related behavior in rodents. Nature Protocol. 2007; 2: 322–328. 17- Broadbent NJ, Gaskin S, Squire LR, Clark RE. Object recognition memory and the rodent hippocampus. Learn Mem. 2009; 17: 5-11. 18- Ennaceur A,Delacour J.A new one-trial test for neurobiological studies of memory in rats. 1: Behavioral data. Behav Brain Res. 1988; 31: 47-59. 19- Zhanga R, Xuea G, Wanga S, Zhanga L, Shia C,Xie X. A novel object recognition as a facile behavior test for evaluating drug effects in aPP/PS1 Alzheimer’s disease Mouse Model. J Alzheimer’s Diseas. 2012; 31: 801–812. 20- Al-Snafi AE. The Pharmacological Importance of Bauhinia variegata. A Review. International J Pharmaceut Sci Res. 2013; 4: 160-164. 21- Ambasta, S. P. The wealth of India, Raw materials. Publication and information directorate, CSIR Delhi. 1998. 22- Kumar YR, Rajani GP. Analgesic and anti-ulcer activities of ethanol and aqueous extracts of root of Bauhinia variegata Linn. Int J Pharmacol. 2011; 1-7. 23- Marasani A, Kavitha CN, Babu SM.Antistress/adaptogenic activity of Bauhinia variegata against different stress paradigms. Int J Pharmaceut Biol Arch. 2013; 4: 956-964. 24- Rajani GP, Ashok P.In vitro antioxidant and antihyperlipidemic activities of Bauhinia variegata Linn. Ind J Pharmacol. 2009; 41: 227-232. 25- Sathya B, Ariharasivakumar G, Vimalson DC, Subramani M, Magesh M. Psychopharmacological evaluation of ethanolic extract of leaves of Bauhinia Tomentosa L in mice. Int J Pharma Technol. 2011; 3: 3693-3709. 26- Cavalcanti EM, Bonafé C, Silva MG,Gerenutti M. Properties of Bauhinia forficata LIK in rats: Behavioral evaluations. Pharmacology Online, 2011; 2: 205-211. 27- Davey MS, Atlee WC, Bharathi A,Farook M.Antianxiety effect of methanolic extract of Bauhinia racemosa [lamk] stem bark in mice. Int J Pharma Bio Sci. 2011; 2: 217-224. 28- Borges dos Santos FJ, Gonçalo de Lima S, Cerqueira GS, Citó AMGL, CavalcanteAACM, Marques THC, de Freitas RM.Chemical composition and anxiolytic-like effects of the Bauhinia platypetala. Braz J Pharmacog. 2012; 22: 507-516. 29- RoaKoteswara Y, Shih-Hua F, Yew-Min T.Anti-inflammatory activity of flavonoids and triterpene caffeate isolated from Bauhinia variegata. Phytother Res. 2008; 22: 957-962. 30- Vauzour D, Vafeiadou K, Rodriguez-Mateos A, Rendeiro C, Spencer JPE.Theneuroprotective potential of flavonoids: a multiplicity of effects. Genes Nutrit. 2008; 3: 115-126.


Refbacks

  • There are currently no refbacks.
';



Copyright (c) 2017 Furhan Iqbal, Muhammad Jahangir, Asmat Ullah, Muhammad Naseer Khan Khattak, Shahid Iqbal

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Advanced Research Journals

4/70-71. Black Well HB, ALW 30100 IND

Copyright@arjournals.org (Design) 2009-2017

 

Follow @arjournals on Twitter