Estrogenic and anxiolytic effects of the decoction of stem bark of Khaya anthotheca (Welw.) C.DC (Meliaceae) in ovariectomised Wistar rats

Germain Jean Magloire Ketcha Wanda, Franklin ZEMO GAMO, Sefirin DJIOGUE, Charline Florence AWOUNFACK, Dieudonne NJAMEN

DOI: http://dx.doi.org/10.5138/09750185.2033

Abstract


Khaya anthotheca (Welw.) C.DC (Meliaceae) is a plant used in Cameroon to alleviate vaginal dryness in postmenopausal women and is also known to have anxiolytic properties.This work was designed to evaluate estrogen-like effects of this plant on primary estrogens targets of ovariectomized adult rats, as well as to evaluate its anxiolytic activities in the elevated plus-maze (EPM) test. In the 3-day uterotrophic assay, the extract increased (p < 0.01) the size of the vaginal epithelia and stimulated the acini differentiation of the mammary gland. In the EPM test, the extract increased the percentage of number of entries (p <0.05; p <0.01) and the percentage of time spent (p < 0.05) into open arms.Italso induced a decrease in percentage of number of entries (p < 0.05; p < 0.01) and the percentage of time spent (p < 0.05) into closed arms. The extract also induced an increase of total arms entries (p < 0.05; p < 0.01) and rearing (p < 0.05). Moreover, there was a decrease of defecation and grooming (p < 0.05; p < 0.01). These results suggest that K. anthotheca is endowed with estrogenic and anxiolytic properties, likely due to the presence of some estrogen-like compounds.


Keywords


Khaya anthotheca, ovariectomy, estrogenic properties, anxiolytic, elevated plus-maze.

References


[1] Pinkerton JV, Stovall DW, Kightlinger RS. Advances in the treatment of menopausal symptoms. Women's Health 2009; 5(4): 361–384.

[2] Doyle BJ, Frasor J, Bellows LE, Locklear TD, Perez A, Gomez- Laurito J, Mahady GB. Estrogenic effects of herbal medicines from Costa Rica used for the management of menopausal symptoms. Menopause 2009; 16(4): 748–755.

[3] World Health Organization. Traditional medicine. Fact sheet 134. 2003-05. Archived from the original 2008; 07–28.

[4] Kinsella K, Velkoff V. An Aging World: 2001. U.S. Census Bureau, Series P95/01-1. U.S. Government Printing Office, Washington, DC 2001.

[5] O’Brien CO. Benzodiazepine use, abuse, and dependence. Journal of Clinical Psychiatry 2005; 66: 28–33.

[6] Albertazzi P. Non-estrogenic approaches for the treatment of climacteric symptoms. Climacteric 2007; 10(2): 115–20.

[7] Umland EM, Cauffield JS, Kirk JK, Thomason TE. Phytoestrogens as therapeutic alternatives to traditional hormone replacement in postmenopausal women. Pharmacotherapy 2000; 20(81): 981–990.

[8] Colditz GA. Estrogen, estrogen plus progestin therapy, and risk of breast cancer. Clincal. Cancer Research 2005; 11(2): 909s–917s.

[9] Low Dog T. Menopause: a review of botanical dietary supplements. The American Journal of Medicine 2005; 118(12b): 98–108.

[10] Ito K. Hormone replacement therapy and cancers: the biological roles of estrogen and progestin in tumorigenesis are different between the endometrium and breast. The Tohoku Journal of Experimental Medicine 2007; 212(1): 1–12.

[11] Scheid V. Traditional Chinese medicine -what are we investigating? The case of menopause. Complementary Therapies in Medicine 2007; 15: 54–68.

[12] Pitkin J. Alternative and complementary therapies for menopause. Menopause International 2012; 18: 20–27.

[13] Ferrari A. Soy extract phytoestrogens with high dose of isoflavones for menopausal symptoms. Journal of Obstetrics and Gynaecology Research 2009; 35: 1083–1090.

[14] Ososki AL, Kennelly EJ. Phytoestrogens: a review of the present state of research. Phytotherapy Research 2003; 17: 845–869.

[15] Toyang NJ, Nuwanyakpa M, Ndi C, Sali Django, Kinyuy WC. Ethnoveterinary medicine practices in the Northwest Province of Cameroon. Indigenous Knowledge and Development Monitor 1995; Regference PRELUDE: VT 08.

[16] Amri E, Kisangau DP. Ethnomedicinal study of plants used in villages around Kimboza forest reserve in Morogoro, Tanzania. Journal of Ethnobiology and Ethnomedicine 2012; 8(1): 1–9.

[17] Ojokuku SA, Okunowo WO, Apena A. Evaluation of the chemical composition of Khaya grandifoliola and Ficus capensis. Journal of Medicinal Plants Research 2010; 4(12): 1126–1129.

[18] Lee Sung-Eun, Kim Mi-Ran, Kim Jeong-Han, Takeoka Gary R, Kim Tae-Wan, Park Byeoung-Soo. Antimalarial activity of anthothecol derived from Khaya anthotheca (Meliaceae). Phytomedicine 2008; 15(6-7): 533-535.

[19] Nfil A, Ndi C, Bayemi PH, Njwe R, Tchoumboue J, Njakoi H, Mopoi N, Njakoi M, Sali-Django. The anthelmintic efficacy of some indigenous plants in the Northwest province of Cameroon. Revue d'élevage et de medicine vétérinaire des pays tropicaux 1999; (52): 103–106.

[20] Njayou FN , Amougou AM , Fouemene TR, Njikam MJ, Sweha R, Bolling B, José EM, Moundipa FP. Antioxidant fractions of Khaya grandifoliola C.DC. and Entada Africana Guill. Et Perr. induce nuclear translocation of Nrf2 in HC-04 cells. Cell Stress Chaperones 2015; 20(6): 991–1000.

[21] Petruczynik A. Analysis of alkaloids from different chemical groups by different liquid chromatography methods. Central European Journal of Chemistry 2012; 10(3): 802–835.

[22] Lane NE, Yao W, Kinney JH, Modin G, Balooch M, Wronski TJ. Both hPTH(1–34) and bFGF increase trabecular bone mass in osteopenic rats but they have different effects on trabecular bone architecture. Journal of Bone and Mineral Research 2003; 18(12): 2105–2115.

[23] OECD. Third meeting of the validation management group for the screening and testing of endocrine disrupters (mammalian effects). Joint meeting of the chemicals committee and the working party on chemical, pesticides and biotechnology. http://www.oecd.org, 2007.

[24] Njamen D, Magne Nde CB, Fomum ZT, Mbanya JC. Preventive effects of an extract of Erythrina Lysistemon (Fabaceae) on some menopausal problems: studies on the rat. Journal of Complementary and Integrative Medicine 2007; 4: 1–7.

[25] Zingue S, Njamen D, Tchoumtchoua J, Halabalaki M , Simpson E, Clyne C, Magne Nde CB. Effects of Millettia macrophylla (Fabaceae) extracts on estrogen target organs of female Wistar rat. Journal of Pharmacological Sciences 2013; 123: 120-131.

[26] Bourin M, Dhonnchadha BA, Colombel M.C, Dib M, Hascoet M. Cyamemazine as an anxiolytic drug on the elevated plus maze and Light/dark paradigm in mice. Behavioral Brain Research 2001; 124(1): 87–95.

[27] Geoffrey BV, Cynthia AM, Mary EC-W, Vicki LC, Galen JC. The Gerbil Elevated Plus-Maze I: Behavioral Characterization and Pharmacological Validation. Neuropsychopharmacology 2002; (27): 357–370.

[28] Adeyemi OO, Akindele AJ, Yemitan OK, Aigbe FR, Fagbo FI. Anticonvulsant, anxiolytic and sedative activities of the aqueous root extract of Securidacalon gepedunculata Fresen. Journal of Ethnopharmacology 2010; 130(2): 191–195.

[29] Ngo Bum E, Soudi S, Ayissi ER, Dong C, Lakoulo NH, Maidawa F, Seke PFE, Nanga LD, Taiwe GS, Dimo T, Njifutie N, Rakotonirina A, Rakotonirina SV, Kamanyi A. Anxiolytic activity évaluation of four médicinal plants from Cameroun. African Journal of Traditional, Complementary and Alternative Medicines 2011; 8: 130–139.

[30] Ngo Bum E, Taiwe GS, Moto FCO, Ngoupaye GT, Nkantchoua GCN, Pelanken MM, Rakotonirina A, Rakotonirina SV. Anticonvulsant, anxiolytic and Sedative properties of the roots of Nauclea latifolia in Mice. Epilepsy and Behavior 2009; 15(4): 434–440.

[31] Casarrubea M, Magnusson MS, Roy V, Arabo A, Sorbera F, Santangelo A, Crescimanno G. Multivariate temporal pattern analysis applied to the study of rat behavior in the elevated plus maze: methodological and conceptual highlights. Journal of Neuroscience Methods 2014; 234: 116–26.

[32] Ketcha GJM, Djiogue S, Zemo GF, Guemnag GS, Njamen D. Anxiolytic and sedative activities of aqueous leaf extract of Dichrocephala integrifolia (Asteraceae) in mice. Journal of Ethnopharmacology 2015; 176: 494–498.

[33] Lund TD, Rovis T, Chung WC, Handa RJ. Novel actions of estrogen receptor-beta on anxiety-related behaviors. Endocrinology 2005; 146(2): 797–807.

[34] Daendee S, Thongsong B, Kalandakanond-Thongsong S. Effects of time of estrogen deprivation on anxiety-like behavior and GABAA receptor plasticity in ovariectomized rats. Behavioural Brain Research 2013; 246: 86–93.

[35] Deepak PS, Kevin MW, Peter P. Insights into Rapid Modulation of Neuroplasticity by Brain Estrogens. Pharmacological Reviews 2013; 65(4): 1318–1350.

[36] Hewitt SC, Deroo BJ, Hansen K, Collins J, Grissom S, Afshari CA, Korach KS. Estrogen receptor-dependent genomic responses in the uterus mirror the biphasic physiological response to estrogen. MolecularEndocrinology 2003; 17(10): 2070–2083.

[37] Wuttke W, Jarry H, Seidlová-Wuttke D. Isoflavones-safe food additives or dangerous drugs? Ageing Research Reviews 2007; 6(2): 150–88.

[38] Njamen D, Djiogue S, Zingue S, Mvondo MA, Nkeh-Chungag BN. In vivo and in vitro estrogenic activity of extracts from Erythrina poeppigiana (Fabaceae). Journal of Complementary and Integrative Medicine 2013; 10: 1–11.

[39] , ME, Chang S, Burrows LJ, Lassmann J, Wein AJ, Moreland RS, Chacko SK. Effect of estrogen on molecular and functional characteristics of the rodent vaginal muscularis. Journal of Sexual Medicine 2013; 10(5): 1219–1230.

[40] Deecher DC, Dorries K. Review Understanding the pathophysiology of vasomotor symptoms (hot flushes and night sweats) that occur in perimenopause, menopause, and postmenopause life stages. Archives Womens Mental Health 2007; 10: 247–57.

[41] Weihua Z, Andersson S, Cheng G, Simpson ER, Warner M, Gustafsson JA. Update on estrogen signalling. FEBS Lett 2003; 546(1): 17–24.

[42] Odum J, Lefevre PA, Tittensor S, Paton D, Routledge EJ, Beresford NA, Sumpter JP, Ashby J. The rodent uterotrophic assay: critical protocol features, studies with nonyl phenols, and comparison with a yeast estrogenicity assay. Regulatory Toxicology and Pharmacology 1997; 25(2): 176–18.

[43] Maribel H-R, Adolfo G-C, Alejandro Z, Enrique J-F, Maira H-R, Victor MN-G. The standardized extract of Loeseli amexicana possesses anxiolytic activity through the γ-amino butyric acid mechanism. Journal of Ethnopharmacology 2011; 138: 261–267.

[44] Zhang Z. Therapeutic effects of herbal extracts and constituents in animal models of psychiatric disorders. Life Sciences 2004; 75(14): 1659–1699.

[45] Rodgers RJ, Dalvi A. Anxiety, defense and the elevated plus-maze. Neuroscience and Biobehavioral Reviews 1997; 21(6): 801–810.

[46] Thakur VD, Mengi SA. Neuropharmacological profile of Eclipta alba (Linn.) Hassk. Journal of Ethnopharmacology 2005; 102(1): 23–31.

[47] Oviedo VM, Milded GG, Rincon J, Guerrero MF. Effect of an extract of Annona muricata on central nervous system. Pharmacologyonline 2006; 3: 342–347.

[48] Grundmann O, Nakajima J -I, Seo S, Butterweck V. Anti-anxiety effects of Apocynum venetum L. in the elevated plus maze test. Journal of Ethnopharmacology 2007; 110(3): 406–411.

[49] Janaine MC, Renata L, Lucas G, João CPM and Rúbia MWO. Preclinical evaluation of Trichilia catigua extracts on the central nervous system of mice. Journal of Ethnopharmacology 2011; 137(3): 1143–1148.

[50] Walf AA, Frye CA. The use of the elevated plus maze as an assay of anxiety-related behavior in rodents. Nature Protocols 2007; 2(2): 322–328.

[51] Anuradha H, Srikumar BN, Shankaranarayana Rao BS and Lakshmana M. Euphorbia hirta reverses chronic stress-induced anxiety and mediates its action through the GABAA receptor benzodiazepine receptor-Cl2 channel complex. Journal of Neural Transmission 2008; 11(3): 35–42.

[52] Xiu-Yan W, Jing-Yu Y, Jim-Hui W and Chun-Fu w. Anxiolytic effect of saponins from Panax quinquefolium in mice. Journal of Ethnopharmacology 2007; 111: 613-318.

[53] Cheryl SW, Rebecca AA, Kathryn AC, Yow-Jiun J. Estrogens of multiple classes and their role in mental health disease mechanisms. International Journal of Women’s Health 2010; 2: 153–166.


Refbacks

  • There are currently no refbacks.
';



Copyright (c) 2017 Germain Jean Magloire Ketcha Wanda, Franklin ZEMO GAMO, Sefirin DJIOGUE, Charline Florence AWOUNFACK, Dieudonne NJAMEN

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Advanced Research Journals

4/70-71. Black Well HB, ALW 30100 IND

Copyright@arjournals.org (Design) 2009-2017

 

Follow @arjournals on Twitter