Comparative screening of in-vitro free radical scavenging, anti-inflammatory and anti-haemolytic activities from non-polar solvent extracts of Pterocarpus marsupium

Aruna LH, Sanjeevkumar CB, Amarvani PK, Ramesh L Londonkar



Pterocarpus marsupium is widely used as ‘Rasayana’ in ayurvedic system for curing several medical ailments. In view of this, petroleum ether and chloroform extracts of Pterocarpus marsupium bark was scrutinized to unfold free radical scavenging by measuring their capability for scavenging DPPH radical, ABTS, phosphomolybdenum assay as well as reducing power capacity assessment and anti- inflammatory activity by inhibition of protein denaturation. Additionally, RBC’S were also used to explore the potentiality of Pterocarpus marsupium different extracts to shield RBC in oxidative stress condition. Chloroform extract showed an effective pharmacological activity in all assays when compared with petroleum ether extract and their respective standards. This reveals the use of Pterocarpus marsupium as a potent source of antioxidant, anti-inflammatory, anti-haemolytic agent and would provide an ample opportunity for further investigation. Isolation of active compounds from this plant responsible for producing such bioactivities is under process.


Pterocarpus marsupium, antioxidant activity, anti-inflammatory activity, anti-haemolytic activity, petroleum ether extract, chloroform extract

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[1]. Valko M, Leibfritz D, Moncol J, Cronin M.T.D,Mazur M, Telser J, Int. J. Biochem. Cell Biol., 2006, 7, 45.

[2]. Chaitanya KV. Role of oxidative stress in human health: an overview. j pharmacy res, 2010; 3(6).

[3]. Aditi Sharma, Rohit Goyal , Lalit Sharma, Potential biological efficacy of Pinus plant species against oxidative, inflammatory and microbial disorders, BMC Complementary and Alternative Medicine,2016, 16:35

[4]. Umarani N, Ilango K. Exploration of in vitro antioxidant and antimicrobial studies of Momordica cymbalaria fenzyl leaves extracts, International Journal of Pharmacy and Pharmaceutical Sciences ,2015,7(3)

[5]. Naima Saeed, Muhammad R Khan, Maria Shabbir , Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L,BMC Complementary and Alternative Medicine 2012, 12:221

[6]. Humaira Yasmeen, Shahida Hassnain. Comparative analysis of different bioactivities of Curcuma longa, Nigella sativa Seeds and Camellia sinensis extracted by four different methods: A green way to reduce oxidative stress, Food Sci. Biotechnol, 2016; 25(3): 811-819

[7]. Prajapati ND, Purohit SS, Sharma AK and Kumar TA. Handbook of medical plants, Agrobiosis, 1st ed, 2000, 429

[8]. Mahnaaz Mohammadi, Swati Khole, Thomas Paul Asir Devasagayam, Saroj S. Ghaskadbi, Pterocarpus marsupium extract reveals strong in vitro antioxidant activity, Drug Discov Ther. 2009; 3(4):151-161.

[9]. Khem R. Joshi, Hari P. Devkota, Shoji Yahara, Chemical Analysis of Heartwood of Bijayasal (Pterocarpus marsupium Roxb.),Nepal Journal of Science and Technology,2012, 13(2): 219-224

[10]. Haque A, In vitro evaluation of phytochemical profile and antioxidant effect of chloroform and petroleum ether extracts of Dendrophthoe falcata (Linn) leaves, Pharmacologyonline, 2015, 2:6-13

[11]. Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal. Biochem. 269 (1999) 337–341

[12]. Izabela Biskup, Iwona Golonka, Andrzej Gamian, Zbigniew Sroka, Antioxidant activity of selected phenols estimated by ABTS and FRAP methods, Postepy Hig Med Dosw, 2013; 67: 958-963

[13]. Parisa Faraji, Rashid Jamei,The Study of Phenolic Compounds Antioxidant Activity in Methanolic and Aqueous Extracts of Several Plant Species of Urmia Lake Margin, International Journal of Pharmacognosy and Phytochemical Research, 2016, 8(2),289-296

[14]. Asha Tukappa NK, Ramesh L. londonkar, Sanjeev Kumar CB, Hanumantappa B Nayaka, Evaluation of in vitro and in vivo anti-inflammatory and toxicity studies of methanolic extract of Rumex vesicarius Linn, Orient Pharm Exp Med,2015.

[15]. Chidambaram Kumarapppan, Albert Jaswanth, Karpagam Kumarasunderi, Antihaemolytic and snake venom neutralizing effect of some Indian medicinal plants, Asian Pacific Journal of Tropical Medicine, 2011; 743-747

[16]. Juan X, Shubing C, Qiuhui H, Antioxidant activity of brown pigment and extracts from black sesame seed (Sesamum indicum L.), Food Chem,2005, 91,79–83.

[17]. Perumalsamy Muneeswari, Subburaj Deepika, Palanisamy Chella Perumal, Velliyur Kanniappan Gopalakrishnan , Kannappan Poornima, Phytochemical Screening and Free Radical Scavenging Activity of Chloroform Extract of Sida acuta Burm. F. International Journal of Pharmacognosy and Phytochemical Research 2016; 8(4); 663-667

[18]. Mohammad Shahriar ,Md. Ismail Hossain ,Farzana Anwar Sharmin ,Sadika Akhter, Md. Aminul Haque ,Mohiuddin Ahmed Bhuiyan, InVitro Antioxidant and Free Radical Scavenging Activity of Withania Somnifera Root, Iosr Journal Of Pharmacy,2013,3(2): 38- 47

[19]. Mortada Mohamed El-Sayed, Maher Mahmoud El-Hashash, Heba Raafat Mohamed, Ezzat El-Sayed Abdel-Lateef, Phytochemical Investigation and in vitro Antioxidant Activity of Different Leaf Extracts of Salix mucronata Thunb,Journal of Applied Pharmaceutical Science, 2015, 5 (12):080-085.

[20]. Sanjeevkumar CB, Ramesh L Londonkar, Umesh Madire Kattegouda, Asha Tukappa N K. Screening of In vitro Antioxidant Activity of Chloroform Extracts of Bryonopsis laciniosa Fruits, International Journal of Current Microbiology and Applied Sciences, 2016, 5(3): 590-597

[21]. G. Shobana, K. Keerthana, Dr.N.Agnel Arul John, A.Edwina Sherley Felicita, In vitro antioxidant potentials of aqueous extract of Anacardium occidentale L, World Journal of Pharmacy and Pharmaceutical Sciences, 2106,5(1) :1458-1467

[22]. Nazia Hoque, Md. Hossain Sohrab, Trisha Debnath, Md. Sohel Rana. Antioxidant, antibacterial and cytotoxic activities of various extracts of Thysanolaena maxima (Roxb) Kuntze available in Chittagong hill tracts of Bangladesh.International Journal of Pharmacy and Pharmaceutical Sciences 2016;8(7):168-172.

[23]. Eimad dine Tariq Bouhalali, Khalid Sellam, Mohamed Bammou, Chakib Alem, Younes Filali-Zehzouti, In vitro Antioxidant and anti-inflammatory properties of selected Moroccan medicinal plants. Journal of Applied Pharmaceutical Science, 2016; 6 (05):156-162.

[24]. Arzanlou M, Bohlooli S, Jannati E, Mirzanejad-Asl H. Allicinfrom garlic neutralizes the hemolytic activity of intra- and extracellular pneumolysin O in vitro. Toxicon 2011; 57: 540-545

[25]. Oyedapoo OO, Akinpelu BA, Akinwunmi KF, Adeyinka MO, and Sipeolu FO. Int J Plant Physiol Biochem 2010; 2(4): 46-51.


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