Metabolic profile and atherogenic indices of rats treated with Tamarindus indica and Mentha piperita juice.

Sandra Maria Barbalho, Silvia Cristina Cerini Trevisan, Aline Pereira Paes Menezes, Elen Landgraf guiguer, Marie Oshiiwa, Vanessa Sellis, Adriana Fiorini



Changes in diet, physical inactivity and stress contribute to obesity, cardiovascular disease and atherosclerosis. Literature shows that bioactive compounds may result in benefits to human health. The aim of this study was to evaluate the glycemic, lipid profile and atherogenic indices of Wistar rats treated with Mentha piperita and Tamarindus indica juice. Animals received food and water ad libitum and were divided into 3 groups: control group (G1) that received 0.5 mL of water twice daily; Mentha group (G2) treated with 0.5 mL of mint juice and group treated with Tamarindus indica juice (G3),  that received 0.5 mL of tamarind juice twice daily for 40 days. Groups G1, G2 and G3 received the drinks by intragastric rout. After this period the animals were sedated with sodium pentobarbital for blood collection and evaluation of the biochemical profile: total cholesterol, HDL-c, LDL-c, triglycerides and glucose. Atherogenic indexes were also calculated. The results showed a reduction of total cholesterol, LDL-c, triglyceride, body weight and atherogenic indices; and increase in the levels of HDL-c. We may suggest that the use of mint and tamarind juice can positively affect the biochemical parameters and reduce the atherogenic indexes of Wistar rats.


Tamarindus indica, Mentha piperita, glycemia, cholesterol, triglycerides, HDL-c.

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Radigonda B, Souza RKT, Junior LC, Silva AMR. Avaliação do acompanhamento de pacientes adultos com hipertensão arterial e ou diabetes melito pela Estratégia Saúde da Família e identificação de fatores associados, Cambé-PR, 2012. Epidemiol. Serv. Saúde 2016 Jan/Mar; 25(1): 115-126. 2. Bortoletto MSS, Souza RKT, Cabrera MAS, González AD. Metabolic syndrome, components and associated factors in adults aged 40 years or older from a city in southern Brazil. Cad. Saúde Colet 2016 Apr; 24 (1): 32-40. 3. Ribak PA, Ghisleni CP, Zemolin GP, Zanardo VPS. Estado nutricional, consumo de ácidos graxos e sua relação com o perfil lipídico de pacientes ambulatoriais. Perspectiva 2015; 40(14): 85-95. 4. Badal RM, Badal D, Badal P, Khare A, Shrivastava J, Kumar V. Pharmacological Action of Mentha piperita on Lipid Profile in Fructose-Fed Rats. Iran J Pharm Res. 2011 Autumn; 10(4): 843–848. 5. Figueroa-Pérez MG, Gallegos-Corona MA, Ramos-Gomes M, Reynoso-Camacho R. Salicylic acid elicitation during cultivation of the peppermint plant improves anti-diabetic effects of its infusions. Food Funct 2015 Jun; 6(6): 1865-1874. 6. David EFS, Mischan MM, Marques MOM, Boaro CSF. Physiological indexese macro- and micronutrients in plant tissue and essential oil of Mentha piperita L. grown in nutrient solution with variation in N, P, K and Mg levels. Rev. Bras. Pl. Med 2014 Mar; 16(1): 97-106. 7. Barbalho SM, Damasceno DC, Spada AP, Silva VS, Martuchi KA, Oshiiwa M, Machado FM, Mendes CG. Metabolic Profile of Offspring from Diabetic Wistar Rats Treated with Mentha piperita (Peppermint). Evid Based Complement Alternat Med 2011 Apr; 430237. 8. Johari NZ, Ismail IS, Sulaiman MR, Abas F, Shaari K. Acute toxicity and metabolomics analysis of hypocholesterolemic effect of Mentha piperita aqueous extract in Wistar rats. Int. J. Appl. Res. Nat. Prod 2015 Jan; 8(1): 1–11. 9. Menezes APP, Trevisan SCC, Barbalho SM, Guiguer EL. Tamarindus indica L. A plant with multiple medicinal purposes. Journal of Pharmacognosy and Phytochemistry 2016 Apr; 5(3): 50-54. 10. Escalona-Arranz JC, Garcia-Diaz J, Perez-Rosés R, Vega J, Rodriguez-Amado J, Morris-Quevedo HJ. Effect of Tamarindus indica L. leaves' fluid extract on human blood cells. Nat Prod Res 2014; 28(18): 1485-1488. 11. Bhadoriya SS, Ganeshpurkar A, Narwaria J, Rai G, Jain AP. Tamarindus indica: Extent of explored potential. Pharmacogn Rev 2011 Jan; 5(9): 73-81. 12. Jindal V, Dhingra D, Sharma S, Parle M, Harna RK. Hypolipidemic and weight reducing activity of the ethanolic extract of Tamarindus indica fruit pulp in cafeteria diet – and sulpiride – induced obese rats. Pharmacol Pharmacother 2011 Apr-Jun; 2(2): 80-84. 13. De Caluwé E, Halamov K, Van Damme P. Tamarindus indica L.: a review of traditional uses, phytochemistry and pharmacology. Afrika Focus 2010; 23(1): 53-83. 14. Paula FS, Kabeya LM, Kanashiro A, Figueiredo AS, Azzolini AE, Uyemura SA. Lucisano-Valim YM. Modulation of human neutrophil oxidative metabolism and degranulation by extract of Tamarindus indica L. fruit pulp. Food Chem Toxicol 2009; 47(1): 163-170. 15. Ahmadvand H, Bagheri S, Tamjidi-Poor A, Cheraghi M, Azadpour M, Ezatpour B, Moghadam S, Shahsavari G, Jalalvand M. Biochemical effects of oleuropein in gentamicin-induced nephrotoxicity in rats. ARYA Atheroscler 2016 Mar; 12(2): 87-93. 16. Munshi RP, Joshi SG1, Rane BN. Development of an experimental diet model in rats to study hyperlipidemia andinsulin resistance, markers for coronary heart disease. Indian J Pharmacol 2014; 46(3): 270-276. 17. Ikewuchi, JC. Alteration of plasma biochemical, haematological and ocular oxidative indices of alloxan induced diabetic rats by aqueous extract of Tridax procumbens Linn (Asteraceae). Excli Journal 2012; 11: 291–308. 18. Barbalho SM, Bechara MD, Tofano RJ, Quesada K, Mendes CG, Oshiiwa M. Metabolic syndrome and C reactive protein in patients undergoing angiography: Inevitable association?. Diabetes Metab Syndr 2016; 18. Pii: S1871-4021(16)30090-X. 19. Yousuf B, Gul K, Wani AA, Singh P. Health Benefits of Anthocyanins and Their Encapsulation for Potential Use in Food Systems: A Review. Crit Rev Food Sci Nutr 2016 Oct 2;56(13):2223-30. 20. Müller L, Caris-Veyrat C, Lowe G, Böhm V. Lycopene and Its Antioxidant Role in the Prevention of Cardiovascular Diseases-A Critical Review. Crit Rev Food Sci Nutr 2016 Aug 17; 56(11):1868-1879. 21. Patil SR, Patial RS, Godghate AG. Mentha piperita Linn: Phytochemical, antibacterial and dipterian adulticidal approach. International Journal of Pharmacy and Pharmaceutical Sciences 2016; 8(3): 352-355. 22. Johari NZ, Ismail IS, Sulaiman MR, Abas F, Shaari K. Acute toxicity and metabolomics analysis of hypocholesterolemic effect of Mentha piperita aqueous extract in Wistar rats. Int. J. Appl. Res. Nat. Prod 2015; 8(1): 1–11. 23. Mallick B, Sinha S, ROY D. Evaluation of antioxidative potential of field grown and tissue culture derived Mentha piperita L. plants. Int. J. Curr. Microbiol. App. Sci 2016; 5(3): 382-391. 24. Liu X, Sun ZL, Jia AR, Shi YP, Li RH, Yang PM. Extraction, preliminary characterization and evaluation of in vitro antitumor and antioxidant activities of polysaccharides from Mentha piperita. Int J Mol Sci. 2014; 15;15(9): 16302-19. 25. Sharafi SM, Rasooli I, Owlia P, Taghizadeh M, Astaneh SD. Protective effects of bioactive phytochemicals from Mentha piperita with multiple health potentials. Pharmacogn. Mag 2010; 6, 147–153. 26. Barbalho SM, Spada APM, Oliveira EP, Paiva-Filho ME, Martuchi KA, Leite NC, Deus RM, Sasaki V, Braganti LS, Oshiiwa M. Mentha piperita effects on wistar rats plasma lipids. Brazilian Archives of Biology and Technology, 2009; 52(5): 1137-1143. 27. Amir M, Khan MA, Ahmad S, Akhtar M, Mujeeb M, Ahmad A, Khan SA, Al-Abbasi FA. Ameliorating effects of Tamarindus indica fruit extract on anti-tubercular drugs induced liver toxicity in rats. Nat Prod Res 2016; 30(06): 715-719. 28. Sharma R, Kamboj S, Khurana R, Singh G, Rana V. Physicochemical and functional performance of pectin extracted by QbD approach from Tamarindus indica L. pulp. Carbohydr Polym 2015; 134: 364-374. 29. Lim CY, Mat Junit S, Abdulla MA, Abdul Aziz A. In vivo biochemical and gene expression analyses of the antioxidant activities and hypocholesterolaemic properties of Tamarindus indica fruit pulp extract. PLoS One 2013; 8(7): e70058. 30. Azman KF, Amom Z, Azlan A, Esa NM, Ali RM, Shah ZM, Kadir KK. Antiobesity effect of Tamarindus indica L. pulp aqueous extract in high-fat diet-induced obese rats. J Nat Med 2012; 66(2):333-342. 31. Paula FS. Efeitos do extrato da polpa do fruto de Tamarindus indica L. sobre funções efetoras de neutrófilos humanos ativados. Dissertação de Mestrado. Ribeirão Preto: Universidade de São Paulo, 2007, 131. 32. Martinello F, Soares SM, Santos AC, Sugohara A, Garcia SB, Curti C, Uyemura AS. Hypolipemic and antioxidant activities from Tamarindus indica L. pulp fruit extract in hypercholesterolemic hamsters. Food Chem Toxicol 2006; 44(6): 810-818. 33. Sasidharan SR, Joseph JA, Anandakumar S, Venkatesan V, Madhavan CN, Agarwal A. Ameliorative potential of Tamarindus indica on high fat diet induced nonalcoholic fatty liver disease in rats. Scientific World Journal 2014. 34. Pinar Kuru. Tamarindus indica and its health related effects. Asian Pac J Trop Biomed 2014; 4(9):676-681. 35. Sudjaroen Y, Haubner R, Würtele G, Hull WE, Erben G, Spiegelhalder B, Changbumrung S, Bartsh H, Owen RW. Isolation and structure elucidation of phenolic antioxidants from Tamarind (Tamarindus indica L.) seeds and pericarp. Food Chem Toxicol 2005; 43(11): 1673-1682. 36. Ali N, Shah S. Spasmolytic activity of fruits of Tamarindus indica L. Young Pharm, 2010; 2(3): 261-264. 37. Razali N, Abdul Aziz A, Lim CY, Mat Junit S. Investigation into the effects of antioxidant-rich extract of Tamarindus indica leaf on antioxidant enzyme activities, oxidative stress and gene expression profiles in HepG2 cells. PeerJ 2015; Oct 1;3:e1292. 38. Ikewuchi CJ, Ikewuchi CC. Alteration of Plasma Lipid Profiles and Atherogenic Indices by Stachytarpheta jamaicensis check for this species in other resources L. (Vahl). Biokemistri 2009; 21(2): 71–77. 39. Frohlich J, Dobiásová M. Fractional esterification rate of cholesterol and ratio of triglycerides to HDL-cholesterol are powerful predictors of positive findings on coronary angiography. Clin Chem 2003; 49(11): 1873–1880. 40. Natukunda S, Muyonga JH, Mukisa IM. Effect of tamarind (Tamarindus indica L.) seed on antioxidant activity, phytocompounds, physicochemical characteristics, and sensory acceptability of enriched cookies and mango juice. Food Sci Nutr 2015; Nov 18;4 (4): 494-507. 41. Soradech S, Petchtubtim I, Thongdon-A J, Muangman T. Development of Wax-Incorporated Emulsion Gel Beads for the Encapsulation and Intragastric Floating Delivery of the Active Antioxidant from Tamarindus indica L.


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