Immunomodulatory and antiarthritic activities of Smilax zeylanica

P Veeresh Babu, T Ashwini, M. Vamsi Krishna, M. Ganga Raju

DOI: http://dx.doi.org/10.5138/09750185.1963

Abstract


Smilax zeylanica Linn has been traditionally used in the treatment of rheumatoid arthritis but, no scientific data has been published supporting the claimed ethanomedical use. This study was designed to investigate the immunomodulatory and antiarthritic activities of Smilax zeylanica.

Methanolic extract of Smilax zeylanica (MESZ) roots was tested for its immunomodulatory activity by NBT reduction test. Antiarthritic activity of the same was tested by in vitro protein denaturation and in vivo complete Freunds adjuvant (CFA) induced arthritis.

MESZ showed its significant effect on both cell mediated and humoral immunity to suppress stimulated immune responses in NBT reduction test . It also markedly inhibited protein denaturation in in vitro model. Extract at 200 mg/kg and 400 mg/kg showed statistically significant inhibition ( p<0.05) of the edema formation in CFA model. Histopathological studies of ankle joints also supported this finding.

The presence of steroids in the extract might be responsible for the prominent immunomodulatory and antiarthritic activities of the plant. Hence the present study concluded that Smilax zeylanica holds immunomodulatory and antiarthritic activities.

Keywords


Smilax zeylanica Linn, Steroids, Immunomodulatory activity, Antiarthritic activity.

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References


1. Gabriel SE, Crow son CS, Kremers HM. Survival in rheumatoid arthritis population-based analysis of trends over 40 years. Arthritis Rheum. 2003; 48: 54-8.

2. Hotchkiss RS, Nicholson DW. Apoptosis and caspases regulate death and inflammation in sepsis. Nat Rev Immunol. 2006; 6(11): 813-22.

3. Mahesh Kaneria, S.R. Naik, R.K. Kohli. Anti inflammatory, anti arthritic and analgesic activity of a herbal formulation. Indian J Exp Biol. 2007; 45: 278-284.

4. Serhat Keser, Sait Celik, Semra Turkoglu, Ökkes Yilmaz, Ismail Turkoglu. Hydrogen Peroxide Radical Scavenging and Total Antioxidant Activity of Hawthorn. Chem J. 2012; 2(1): 9-12.

5. Bhalerao AR, Desai SK, Serathia BR, Vartak KM, Doshi GM. Antiarthritic studies on Nyctanthes arbortristis and Maharasnadi ghan. Scholars Res Libr. 2011; 3(4): 101- 110.

6. Natarajan P, Kala R, Sumi john, Thangathirupathi A. Hypolipidemic activity of hydroalcoholic extract of Smilax zeylanica Linn. root on cholesterol diet induced hyperlipidemic rats. World J Pharmacy and Pharm Sciences. 2015; 4(11): 1371-1379.

7. O’Dell J, Paulsen G, Haire C, Gerald Moore F. Treatment of early seropositive rheumatoid arthritis with minocycline: Four year followup of a double-blind, placebo-controlled trial. Arthritis Rheum. 1999; 42(8): 1691-1695.

8. Goekoop-Ruiterman YPM, de Vries-Bouwstra JK, Allaart CF, Van Zeben D, Kerstens PJSM, Hazes JMW, Zwinderman AH, Ronday HK, Han KH, Westedt ML, Gerards AH, Van Groenendael JHLM, Lems WF, Van Krugten MV, Breedveld FC, Dijkmans BAC. Clinical and radiographic out- comes of four different treatment strategies in patients with early rheumatoid arthritis (the Best Study): A randomized controlled trial. Arthritis Rheum. 2005; 52(11): 3381-3390.

9. Sheelarani T, Gopal, Seetha Lakshmi S, Chitra K. In vitro antiarthritic and anti-inflammatory activity in aerial parts of Myxopyrum Serratulum A.W Hill. Int J Pharm Sci Rev Res. 2014; 28(2): 162-163.

10. Serhat Keser, Sait Celik, Semra Turkoglu, Ökkes Yilmaz, Ismail Turkoglu. Hydrogen Peroxide Radical Scavenging and Total Antioxidant Activity of Hawthorn. Chem J. 2012; 2(1): 9-12.

11. Thakur M, Bhargava S, Dixit VK. Immunomodulatory activity of Chlorophytum borivilianum Sant. F. Evid Based Complement Alternat Med. 2006; 4: 419-423.

12. Genovese MC, Becker JC, Schiff M, Luggen M, Sherrer Y, Kremer J, Birbara C, Box J, Natarajan K, Nuamah I, Li T, Aranda R, Hagerty DT, Dougados M. Abata- cept for rheumatoid arthritis refractory to tumor necrosis factor inhibition. N Engl J Med 2005; 353(11): 1114-1123.

13. Srikumar R, Jeya Parthasarathy N, Sheela Devi R. Immunomodulatory activity of Triphala on neutrophil functions. Biol Pharm Bull. 2005; 28: 1398-1403.

14. Jones R, Moreland L. Tumor necrosis factor inhibitors for rheumatoid arthritis. Arthritis Foundation. 2009; 48(3): 1-4.

15. Vane JR, Botting RM. New insights into the mode of action of anti-inflammatory drugs. Inflamm Res. 1995; 44(1): 1-10.

16. Serhat Keser, Sait Celik, Semra Turkoglu, Ökkes Yilmaz, Ismail Turkoglu. Hydrogen Peroxide Radical Scavenging and Total Antioxidant Activity of Hawthorn. Chem J. 2012; 2(1): 9-12.

17. Pathak N, Gohil P, Patel NB, Kasture S, Jivani N, Bhalodia Y. Curative effect of Albizia lebbeck methaanolic extract against adjuvant arthritis-with special reference to bone erosion. Int J Pharma Sci Drug Res. 2009; 1: 183-187.

18. Surendra Agarwal S, Saurabh Khadase C, Gokul Telele S. Studies on Immunomodulatory activity of Capparis zeylanica leaf extracts. Int J Pharm Sci Nanotech. 2010; 3(1): 887-903.

19. Amal K. Maji, Subhadip Mahapatra, Debdulal Banerjee. In vivo immunomodulatory potential of standardized Pueraria tuberosa extract and its isoflavanoids. Int J Pharm Pharm Sci. 2014; 6: 0975-1491.

20. Coico R, Sunshine G, Benjamini E. Immunology: A short course. 5th Ed. John Wiley & Sons, Inc., New Jersey; 2003.

21. Heck L, Koopman W. Biologic agents for treating rheumatoid arthritis. Concepts and progress. Arthritis Rheum. 2007; 40(3): 397-409.


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